CAJ | 학술논문

目的构建人肿瘤裸鼠移植瘤模型,探讨多亮氨酸重复区免疫球蛋白样蛋白3(LRIG3)表达沉默后对移植瘤生长以及表皮生长因子受体(EGFR)表达的影响.方法将Hela229细胞接种于裸鼠背部皮下,成瘤后将裸鼠分为3组,分别在瘤体内注射生理盐水、LRIG3反义寡核苷酸(ASODN)、LRIG3错义寡核苷酸(MSODN),检测各组移植瘤生长以及LRIG3和EGFR表达.结果 ASOND组较其他两组瘤体生长加快、体积增大,苏木素-伊红(HE)染色可见核仁染色较深,核分裂相增多,LRIG3蛋白表达结果:空白对照组为0.2302±0.0108、MSODN组为0.2203±0.0183,ASODN组为0.1532±0.0132,两两比较,ASODN组肿瘤组织中LRIG3蛋白表达明显降低,差异有统计学意义(P＜0.05).EGFR蛋白表达结果:空白对照组为0.9212±0.0796、MSODN组为0.9904±0.0830、ASODN组为1.2730±1.1116,两两比较,ASODN组肿瘤组织中EGFR蛋白表达明显增加,差异有统计学意义(P＜0.05).LRIG3表达降低,EGFR表达升高.结论 LRIG3基因表达抑制引起人肿瘤裸鼠移植瘤生长加快,瘤体内EGFR表达升高.
목적 구건인종류라서이식류모형,탐토다량안산중복구면역구단백양단백3(LRIG3)표체침묵후대이식류생장이급표피생장인자수체(EGFR)표체적영향.방법 장Hela229세포접충우라서배부피하,성류후장라서분위3조,분별재류체내주사생리염수、LRIG3반의과핵감산(ASODN)、LRIG3착의과핵감산(MSODN),검측각조이식류생장이급LRIG3화EGFR표체.결과 ASOND조교기타량조류체생장가쾌、체적증대,소목소-이홍(HE)염색가견핵인염색교심,핵분렬상증다,LRIG3단백표체결과:공백대조조위0.2302±0.0108、MSODN조위0.2203±0.0183,ASODN조위0.1532±0.0132,량량비교,ASODN조종류조직중LRIG3단백표체명현강저,차이유통계학의의(P＜0.05).EGFR단백표체결과:공백대조조위0.9212±0.0796、MSODN조위0.9904±0.0830、ASODN조위1.2730±1.1116,량량비교,ASODN조종류조직중EGFR단백표체명현증가,차이유통계학의의(P＜0.05).LRIG3표체강저,EGFR표체승고.결론 LRIG3기인표체억제인기인종류라서이식류생장가쾌,류체내EGFR표체승고.
Objective Leucine-rich repeats and Immunoglobulin-like domains (LRIGs) gene family is a group of recently discovered tumor-associated genes,which acts in a negatingve feedback loop of epidermal growth factor receptor (EGFR) and inhibits the deterioration and proliferation of cancer.The present study investigated the effect of LRIG3 silencing on tumor growth and EGFR expression of the human cervical carcinoma xenografts in nude mice.Methods The Hela229 cells were inoculated subcutaneously into nude mice.The mice were divided into three groups after intratumoral injection of saline,LRIG3 antisense oligodeoxynucleotide (ASODN),and LRIG3 MSODN,respectively.Results Compared to MSODN and saline groups,the tumors in ASOND group grew faster and showed larger size,and demonstrated more deeply stained nucleolus by HE staining.LRIG3 protein expression in the tumors of saline,MSODN and ASODN group were 0.2302 ± 0.0108,0.2203 ± 0.0183 and 0.1532 ± 0.0132,respectively,the expression was significantly down-regulated in ASODN group compared to the other two groups (P ＜ 0.05).EGFR protein expression in the tumors in ASODN group was 1.2730 ± 1.1116,and significantly elevated compared to that in saline and MSODN group,which was 0.9212 ±0.0796 and 0.9904 ±0.0830,respectively (P ＜ 0.05).Conclusion Inhibition of LRIG3 gene expression can accelerate human cervical carcinoma xenograft growth and elevate EGFR expression in xenograft tumors.