CAJ | 학술논문

目的观察小型猪肝硬化模型制备过程中不同病理阶段门静脉自由压的变化.方法同种系巴马小型猪50头(实验组40头,对照组10头),雌雄不限,4个月龄,体质量15～20 kg.实验组予四氯化碳复合因素造模法喂养,正常组予饲料及清水喂养.于造模的0、4、8、12、16、20、24周末开腹行肝组织活检、测量门静脉自由压；统计学分析肝硬化制模过程中不同病理阶段门静脉自由压的变化.结果 24周末,正常对照组获得数据S0期70例次；实验组获得数据So期47例次,轻度肝纤维化(S1+S2期)82例次,重度肝纤维化(S3+S4期)62例次,肝硬化共39例次.正常组、轻度肝纤维化组、重度肝纤维化组及肝硬化组门静脉自由压分别为(4.61±0.84)、(7.74±1.94)、(12.79±4.00)、(17.03±3.41) mmHg(1 mmHg =0.133 kPa),从正常组到肝硬化组,压力的变化表现为逐渐上升的趋势,肝硬化组的压力达最高(P＜0.01).结论四氯化碳复合因素造模法可以成功制备出小型猪肝硬化模型,肝纤维化不同阶段门静脉自由压发生阶段性的变化.
목적 관찰소형저간경화모형제비과정중불동병리계단문정맥자유압적변화.방법 동충계파마소형저50두(실험조40두,대조조10두),자웅불한,4개월령,체질량15～20 kg.실험조여사록화탄복합인소조모법위양,정상조여사료급청수위양.우조모적0、4、8、12、16、20、24주말개복행간조직활검、측량문정맥자유압；통계학분석간경화제모과정중불동병리계단문정맥자유압적변화.결과 24주말,정상대조조획득수거S0기70례차；실험조획득수거So기47례차,경도간섬유화(S1+S2기)82례차,중도간섬유화(S3+S4기)62례차,간경화공39례차.정상조、경도간섬유화조、중도간섬유화조급간경화조문정맥자유압분별위(4.61±0.84)、(7.74±1.94)、(12.79±4.00)、(17.03±3.41) mmHg(1 mmHg =0.133 kPa),종정상조도간경화조,압력적변화표현위축점상승적추세,간경화조적압력체최고(P＜0.01).결론 사록화탄복합인소조모법가이성공제비출소형저간경화모형,간섬유화불동계단문정맥자유압발생계단성적변화.
Objective To assess the free portal pressure changes of the liver during the establishment of mini-pig models of hepatic cirrhosis.Methods Fifty Bama mini-pigs of the same germline,male or female,aged 4 months,weighing 15-20 kg,were included in this study.The mini-pigs were divided at random into an experimental group of 40 and a control group of 10 animals.Compound feed including carbon tetrachloride and phenobarbitone was employed to establish the mini-pig models in the experimental group,and normal feed was given to the control mini-pigs.Liver puncture biopsy was performed and free portal pressures were measured at the end of 0,4th,8th,12th,16th,20th,and 24th week,respectively.Results At the end of 24th week,there were 70 cases of stage 0 hepatic fibrosis in the normal group,while in the experiment group there were 82 cases of slight hepatic fibrosis (stage S1 + S2),62 cases of severe hepatic fibrosis (stage S3 + S4),and 33 cases of hepatic cirrhosis.From the normal to cirrhosis,the free portal pressures were gradually elevated [(4.61 ± 0.84),(7.74 ± 1.94),(12.79 ± 4.00) and (17.03 ±3.41) mmHg (1 mmHg =0.133 kPa)],with statistically significant differences among the different lesion groups (P ＜ 0.01).Conclusion Compound feed including carbon tetrachloride and phenobarbitone can be used to successfully establish mini-pig models of hepatic cirrhosis,showing significant changes of free portal pressures at different stages of the development of hepatic fibrosis.