CAJ | 학술논문

目的观察姜黄素(Cur)对人成骨肉瘤细胞株MG-63增殖的影响和抑制作用.方法不同浓度的Cur作用于体外培养的MG-63细胞,倒置显微镜下观察MG-63细胞的形态学变化,采用噻唑蓝(MTT)法检测不同浓度Cur对MG-63存活率的影响,细胞计数试剂-8(CCK-8)法检测Cur对MG-63细胞增殖抑制能力的影响,膜联蛋白V(Annexin V)/碘化丙锭(PI)双染法标记检测细胞凋亡,黏附实验测定细胞黏附能力,并检测培养液中乳酸脱氢酶(LDH)释放量.结果 MTT法检测经Cur作用后的MG-63细胞存活率以及细胞黏附能力明显下降,LDH的增加提示MG-63细胞死亡率上升；Cur可抑制骨肉瘤细胞株MG-63细胞增殖,50.0 μmol/L的Cur作用12h后,细胞抑制率为47.23％,24 h以后为65.23％.Cur 12.5、25.0、50.0、100.0μmol/L组作用24h后,MG-63细胞的凋亡率分别为(11.24±0.07)％、(28.23±0.35)％、(50.21±1.71)％、(41.22±1.85)％,不同浓度的Cur之间的实验结果差异有统计学意义(P＜0.05).结论 Cur能够抑制MG-63细胞增殖,Cur浓度从12.5 μmol/L增加到25.0 μmol/L,其对MG-63细胞24 h时生长抑制率由(13.56±1.88)％增至(48.27±2.32)％,50.0 μmol/L组24 h生长抑制率为65.23％.50.0μmol/L的Cur对MG-63细胞诱导凋亡作用最明显,作用24 h后,细胞凋亡率为(50.21±1.71)％.
목적 관찰강황소(Cur)대인성골육류세포주MG-63증식적영향화억제작용.방법 불동농도적Cur작용우체외배양적MG-63세포,도치현미경하관찰MG-63세포적형태학변화,채용새서람(MTT)법검측불동농도Cur대MG-63존활솔적영향,세포계수시제-8(CCK-8)법검측Cur대MG-63세포증식억제능력적영향,막련단백V(Annexin V)/전화병정(PI)쌍염법표기검측세포조망,점부실험측정세포점부능력,병검측배양액중유산탈경매(LDH)석방량.결과 MTT법검측경Cur작용후적MG-63세포존활솔이급세포점부능력명현하강,LDH적증가제시MG-63세포사망솔상승；Cur가억제골육류세포주MG-63세포증식,50.0 μmol/L적Cur작용12h후,세포억제솔위47.23％,24 h이후위65.23％.Cur 12.5、25.0、50.0、100.0μmol/L조작용24h후,MG-63세포적조망솔분별위(11.24±0.07)％、(28.23±0.35)％、(50.21±1.71)％、(41.22±1.85)％,불동농도적Cur지간적실험결과차이유통계학의의(P＜0.05).결론 Cur능구억제MG-63세포증식,Cur농도종12.5 μmol/L증가도25.0 μmol/L,기대MG-63세포24 h시생장억제솔유(13.56±1.88)％증지(48.27±2.32)％,50.0 μmol/L조24 h생장억제솔위65.23％.50.0μmol/L적Cur대MG-63세포유도조망작용최명현,작용24 h후,세포조망솔위(50.21±1.71)％.
Objective To investigate the inhibitory effects of curcumin (Cur) on proliferation and apopotosis of human osteosarcoma cell line MG-63 cells.Methods The cultured MG-63 cells were treated with Cur (12.5,25.0,50.0,100.0 μmol/L) for 12 h.The morphologic changes of MG-63 cells were observed by inverted microscope.The survival rate of MG-63 cells was measured by using methyl thiazol tetrazolium (MTT) assay.Cell counting of kit-8 (CCK-8) assay was used to test the growth inhibitory rate.Annexin V/propidium iodide (PI) staining was applied to detect the apoptosis.The survival ratios and the adhesion capacity of MG-63 were detected except the lactate dehydrogenase (LDH) releasing.Results Different concentrations of Cur (12.5,25.0,50.0,100.0 μmol/L) observed in this study have significant effects on the survival ratio of MG-63.The Cur could significantly inhibit the growth of MG-63 in a dose and time dependent manner and induce G2/M Phase cell cycle arrest.After 12 h,cell inhibition rate of 47.23％,24 h after the cell was 65.23％.Cur 12.5,25.0,50.0,100.0 μmol/L group for 24 h,MG-63 cell apoptosis rate was (11.24 ± 0.07)％,(28.23 ± 0.35)％,(50.21 ± 1.71)％ and (41.22 ±1.85) ％,50.0 μmol/L curcumin could induce apoptosis obviously.Conclusion When Cur increased from 12.5 μmol/L to 25.0 μmol/L,its MG-63 cells at 24h growth inhibition rate (13.56 ± 1.88) ％ to (48.27 ±2.32)％,50.0 μmol/L group 24 h growth inhibition rate to 65.23％.50.0 μmol/L of Cur on the MG-63 cells apoptosis induced by the most obvious,for 24 h,apoptosis rate was (50.21 ± 1.71)％.