CAJ | 학술논문

目的探讨肝癌出血性铁过载微环境中Kupffer细胞(KC)促肝癌作用的分子机制.方法采用随机对照方法研究15例合并癌灶出血肝癌患者的标本(出血组),10例癌灶未合并出血肝癌患者的标本(非出血组).采用普鲁士蓝反应(铁染色)检测出血组与非出血组患者的肝癌组织中铁分布；采用流式细胞仪检测KC中活性氧(ROS)的含量,免疫组织化学方法检测肝癌组织中血管内皮生长因子(VEGF)的表达.分析出血性铁过载与KC中ROS含量及肝癌组织中VEGF表达的相关性.结果出血组肝癌组织中KC的ROS含量(46.37±12.94)明显高于非出血组(28.25±11.65,P＜O.05),癌组织中VEGF表达(0.33±0.06)明显高于非出血组(0.21 ±0.04,P ＜0.05),而且铁过载与ROS含量及组织中VEGF表达均呈正相关(r分别为0.706、0.810,P＜0.01).结论肝癌出血性铁过载微环境中KC中ROS含量增加,氧化应激增强,肿瘤生长相关因子VEGF表达升高,是KC促肝癌浸润生长的作用机制.
목적 탐토간암출혈성철과재미배경중Kupffer세포(KC)촉간암작용적분자궤제.방법 채용수궤대조방법연구15례합병암조출혈간암환자적표본(출혈조),10례암조미합병출혈간암환자적표본(비출혈조).채용보로사람반응(철염색)검측출혈조여비출혈조환자적간암조직중철분포；채용류식세포의검측KC중활성양(ROS)적함량,면역조직화학방법검측간암조직중혈관내피생장인자(VEGF)적표체.분석출혈성철과재여KC중ROS함량급간암조직중VEGF표체적상관성.결과 출혈조간암조직중KC적ROS함량(46.37±12.94)명현고우비출혈조(28.25±11.65,P＜O.05),암조직중VEGF표체(0.33±0.06)명현고우비출혈조(0.21 ±0.04,P ＜0.05),이차철과재여ROS함량급조직중VEGF표체균정정상관(r분별위0.706、0.810,P＜0.01).결론 간암출혈성철과재미배경중KC중ROS함량증가,양화응격증강,종류생장상관인자VEGF표체승고,시KC촉간암침윤생장적작용궤제.
Objective To investigate the mechanism of Kupffer cells (KC) promoting hepatoceilular carcinoma (HCC) under haemorrhagic iron overload microenvironment.Methods Fifteen specimens from HCC with haemorrhage (bleeding group) and 10 without haemorrhage (non bleeding group) were collected by using randomized and controlled study.Prussian blue reaction (iron staining) was used to examine the distribution of iron in the tissues of HCC,flow cytometry (FCM) was used to determine the content of reactive oxygen species (ROS) in KC,and immunohistochemistry (streptavidin-biotin peroxidase method) was used to detect the expression of vascular endothelial growth factor (VEGF).The correlation between haemorrhagic iron overload and ROS content in KC as well as VEGF expression in HCC tissues was analyzed.Results The iron staining positive rate in the tissues of bleeding group was significantly higher (0.87 ± 0.21) than that in non bleeding group (0.43 ± 0.15,P ＜ 0.01).The content of ROS in KC of bleeding group (46.37 ± 12.94) was markedly higher than that in non bleeding group (28.25 ± 11.65)(P ＜0.05).The expression of VEGF in the bleeding group (0.33 ±0.06) was obviously higher than in non bleeding group (0.21 ±0.04).Iron staining was positively correlated with the content of ROS in KC and the expression of VEGF in bleeding group (r =0.706 and r =0.810 respectively,P ＜ 0.01).Conclusion HCC with bleeding can cause hemorrhagic iron overload and excessive ferrous ion stress,accompanying with the marked increasing content of ROS in KC and the expression of VEGF in HCC tissues,which may be the mechanism of KC promoting HCC growth and invasion.