CAJ | 학술논문

目的观察乙二醛酶1(GLO1)在胶质瘤中的表达及其对胶质瘤C6细胞增殖迁移及侵袭能力的影响.方法应用免疫组织化学法检测48例胶质瘤标本中GLO1的表达,同时采用小干扰RNA (siRNA)对C6细胞中GLO1的表达进行下调,分别采用Transwell小室及噻唑蓝(MTT)检测GLO1下调对细胞增殖迁移及侵袭能力的影响.结果 GLO1在胶质瘤中的阳性表达率为60.4％,其表达与病理分级有关(P＜0.05)；GLO1表达下调后,C6细胞的增殖迁移及侵袭能力均明显下降(P＜0.05).结论 GLO1的表达与胶质瘤的形成密切相关,其表达下调可明显抑制细胞的增殖迁移及侵袭能力.
목적 관찰을이철매1(GLO1)재효질류중적표체급기대효질류C6세포증식천이급침습능력적영향.방법 응용면역조직화학법검측48례효질류표본중GLO1적표체,동시채용소간우RNA (siRNA)대C6세포중GLO1적표체진행하조,분별채용Transwell소실급새서람(MTT)검측GLO1하조대세포증식천이급침습능력적영향.결과 GLO1재효질류중적양성표체솔위60.4％,기표체여병리분급유관(P＜0.05)；GLO1표체하조후,C6세포적증식천이급침습능력균명현하강(P＜0.05).결론 GLO1적표체여효질류적형성밀절상관,기표체하조가명현억제세포적증식천이급침습능력.
Objective To detect the expression of glyoxalase 1 (GLO1) in gliomas and to investigate its role in migration,proliferation and invasive ability of C6 cell line.Methods GLO1 expression was examined in 48 cases of glioma tissues by using immunohistochemistry.After downregulation of GLO1 expression in C6 cells using small interfering RNA (siRNA) interference fragment,the ability of migration,proliferation and invasion was evaluated by transwell assay and methyl thiazol tetrazolium (MTT) assay,respectively.Results The positive rate of GLO1 in gliomas was 60.4％,and the GLO1 expression was related to the pathological grade (P ＜ 0.05).GLO1 downregulation significantly inhibited migration,proliferation and invasion of C6 cells (P ＜ 0.05).Conclusion GLO1 expression is closely associated with the formation of glioma,and its downregulation can obviously inhibit migration,proliferation and invasion.