中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
2期
259-260
,共2页
许鹤洋%林显敢%罗兴喜%张旸%蓝球生%褚忠华
許鶴洋%林顯敢%囉興喜%張旸%藍毬生%褚忠華
허학양%림현감%라흥희%장양%람구생%저충화
肝癌生磷酸酶-3%丝氨酸蛋白酶抑制剂E3%细胞外调节蛋白激酶
肝癌生燐痠酶-3%絲氨痠蛋白酶抑製劑E3%細胞外調節蛋白激酶
간암생린산매-3%사안산단백매억제제E3%세포외조절단백격매
Phosphatase of regenerating liver-3%Sserine protease inhibitor E3%Extracellular signal-regulated kinase
目的 探讨肝再生磷酸酶-3(PRL-3)对丝氨酸蛋白酶抑制剂E3(serpinE3)的影响及机制,以及PRL-3和serpinF3对结肠癌Lovo细胞侵袭性的影响.方法 通过Western blot方法,分别检测已经稳转入PRL-3载体的Lovo-P细胞和对照组Lovo-C细胞中serpinE3的表达水平,Transwell小室检测Lovo细胞侵袭性.再给予细胞外调节蛋白激酶(ERK)特异性抑制剂U0126(10 μmol/L)预处理Lovo-P细胞6h,观察serpinE3表达的变化,检测Lovo-P细胞侵袭性的改变.结果 Western blot检测结果显示在转染人PRL-3的Lovo-P细胞中,serpinE3表达明显上调,Lovo-P细胞侵袭性增强[(378±13)个比(269±15)个,P<0.05];而当特异性阻断ERK后,Lovo-P细胞中的serpinE3表达下调,并且细胞侵袭性也降低[(21l±9)个比(358±19)个,P<0.05].结论 PRL-3能够通过ERK诱导serpinE3表达上调的方式,增加Lovo细胞的侵袭性.
目的 探討肝再生燐痠酶-3(PRL-3)對絲氨痠蛋白酶抑製劑E3(serpinE3)的影響及機製,以及PRL-3和serpinF3對結腸癌Lovo細胞侵襲性的影響.方法 通過Western blot方法,分彆檢測已經穩轉入PRL-3載體的Lovo-P細胞和對照組Lovo-C細胞中serpinE3的錶達水平,Transwell小室檢測Lovo細胞侵襲性.再給予細胞外調節蛋白激酶(ERK)特異性抑製劑U0126(10 μmol/L)預處理Lovo-P細胞6h,觀察serpinE3錶達的變化,檢測Lovo-P細胞侵襲性的改變.結果 Western blot檢測結果顯示在轉染人PRL-3的Lovo-P細胞中,serpinE3錶達明顯上調,Lovo-P細胞侵襲性增彊[(378±13)箇比(269±15)箇,P<0.05];而噹特異性阻斷ERK後,Lovo-P細胞中的serpinE3錶達下調,併且細胞侵襲性也降低[(21l±9)箇比(358±19)箇,P<0.05].結論 PRL-3能夠通過ERK誘導serpinE3錶達上調的方式,增加Lovo細胞的侵襲性.
목적 탐토간재생린산매-3(PRL-3)대사안산단백매억제제E3(serpinE3)적영향급궤제,이급PRL-3화serpinF3대결장암Lovo세포침습성적영향.방법 통과Western blot방법,분별검측이경은전입PRL-3재체적Lovo-P세포화대조조Lovo-C세포중serpinE3적표체수평,Transwell소실검측Lovo세포침습성.재급여세포외조절단백격매(ERK)특이성억제제U0126(10 μmol/L)예처리Lovo-P세포6h,관찰serpinE3표체적변화,검측Lovo-P세포침습성적개변.결과 Western blot검측결과현시재전염인PRL-3적Lovo-P세포중,serpinE3표체명현상조,Lovo-P세포침습성증강[(378±13)개비(269±15)개,P<0.05];이당특이성조단ERK후,Lovo-P세포중적serpinE3표체하조,병차세포침습성야강저[(21l±9)개비(358±19)개,P<0.05].결론 PRL-3능구통과ERK유도serpinE3표체상조적방식,증가Lovo세포적침습성.
Objective To investigate the influence and mechanisms of phosphatase of regenerating liver-3 (PRL-3) on serine protease inhibitor E3 (serpinE3),and their effects on Lovo cells invasion.Methods Western blotting was used to detect the serpinE3 of Lovo-P and Lovo-C cells.Transwell chamber was used to detect the invasion of Lovo-P and Lovo-C cells.The Lovo-P cells were treated with the extracellular signal-regulated kinase (ERK) inhibitor U0126 (10 μmol/L) for 6 h,and the expression of serpinE3 and invasion of Lovo-P cells were examined.Results The expression of serpinE3 was increased in the Lovo-P cells transfected with human PRL-3.Lovo-P cells exhibited stronger invasion ability than Lovo-Ccells (378 ± 13 vs.269 ± 15,P < 0.05).SerpinE3 was abrogated when Lovo-P treated with U0126 and the invasion ability of the cells was decreased either (211-±9 vs.358 ± 19,P <0.05).Conclusion PRL-3 could induce serpinE3 expression by ERK,and then promotes Lovo cells invasion.