CAJ | 학술논문

目的探讨假基因Cripto-3(Cr-3)在人大肠癌组织中的临床意义.方法收集人大肠癌和正常大肠黏膜组织,采用荧光实时定量聚合酶链反应法检测组织中mRNA水平,分析其表达与患者性别、肿瘤发生部位、分化程度、浆膜侵袭、淋巴结转移及Duke's分期的相关性,并作统计学处理.结果在大肠癌组织中Cr-3阳性率为75.0％ (27/36),在正常大肠黏膜组织中,Cr-3阳性率为0％ (0/36,P＜0.05).进一步发现Cr-3阳性率分别与浆膜层侵袭、淋巴结转移和Duke's分期有关(P＜0.05);而与患者性别、肿瘤发生部位和分化程度无关(P＞0.05).结论假基因Cr-3可能是大肠癌基因诊断和治疗中重要靶点之一.
목적 탐토가기인Cripto-3(Cr-3)재인대장암조직중적림상의의.방법 수집인대장암화정상대장점막조직,채용형광실시정량취합매련반응법검측조직중mRNA수평,분석기표체여환자성별、종류발생부위、분화정도、장막침습、림파결전이급Duke's분기적상관성,병작통계학처리.결과 재대장암조직중Cr-3양성솔위75.0％ (27/36),재정상대장점막조직중,Cr-3양성솔위0％ (0/36,P＜0.05).진일보발현Cr-3양성솔분별여장막층침습、림파결전이화Duke's분기유관(P＜0.05);이여환자성별、종류발생부위화분화정도무관(P＞0.05).결론 가기인Cr-3가능시대장암기인진단화치료중중요파점지일.
Objective To study the clinical significance of pseudogene Cripto-3 (Cr-3) mRNA in human colorectal carcinoma.Methods After human colorectal normal tissues and colorectal tumoral tissues were collected,the mRNA levels of these tissues were determined by real time polymerase chain reaction.Furthermore,the gender,tumor location,and differentiation degree,and serosal invasion,and lymph node metastasis,and Duke's clinical stage were analyzed.Results The positive rates of Cr-3 in colorectal carcinoma was 75％ (27/36),however,in normal colorectal mucosa,the positive rate of Cr-3 was 0％(0/36) (P ＜ 0.05).The positive rates of Cr-3 was closely correlated to the cancer cell invasion,and lymph node metastasis and Duke' s stage (P ＜ 0.05),but the positive rates of Cr-3 was not correlated to gender,tumor location and differentiation degree(P ＞ 0.05).Conclusion Cr-3 gene might be one of the important target of gene diagnosis and gene therapy of colorectal cancer.