CAJ | 학술논문

目的比较不同方式阻断“肠-淋巴”途径,对内毒素、细胞因子和肺功能的影响,探讨“肠-淋巴”途径的作用.方法 SPF级SD大鼠,随机分为5组(n=10):正常对照组(blank);假手术组(Sham);肠道缺血/再灌注组(I/R);肠道缺血/再灌注+淋巴管结扎组(I/R+L)和肠道缺血/再灌注+淋巴引流组(I/R +D).结果 I/R、I/R +L、I/R+D、blank和Sham组血清内毒素水平分别是(0.039 4±0.0025)、(0.0299±0.0039)、(0.028 9±0.003 1)、(0.0252±0.0042)和(0.026 2±0.001 3)Eu/ml;细胞因子分别是肿瘤坏死因子-α(TNF-α):(49.92±7.48)、(36.85±13.46)、(27.67±10.12)、(18.86±12.70)和(25.80±11.19) ng/L;白细胞介素(IL)-6:(41.78 ±9.75)、(19.14±1.48)、(19.20 ±4.14)、(8.69±0.76)和(11.57±2.90) ng/Lo I/R、I/R+L、I/R +D组显著高于blank组和Sham组(P＜0.05);阻断淋巴通路后,I/R+L组和I/R+D组血中的内毒素和细胞因子浓度明显低于I/R组(P＜0.05);I/R+L、I/R +D组的一氧化氮(NO)、总一氧化氮合酶(tNOS)和诱导型一氧化氮合酶(iNOS)浓度与blank组和Sham组比较显著增高(P＜0.05);I/R+L、I/R+D组的NO和I/R+D组的iNOS浓度显著低于I/R组(P＜0.05).结论肠淋巴管结扎或淋巴液引流阻断“肠-淋巴”途径能减轻肠道缺血再灌注导致的局部炎性反应和远隔组织的损伤,淋巴液引流略优于淋巴管结扎,但差异无统计学意义.
목적 비교불동방식조단“장-림파”도경,대내독소、세포인자화폐공능적영향,탐토“장-림파”도경적작용.방법 SPF급SD대서,수궤분위5조(n=10):정상대조조(blank);가수술조(Sham);장도결혈/재관주조(I/R);장도결혈/재관주+림파관결찰조(I/R+L)화장도결혈/재관주+림파인류조(I/R +D).결과 I/R、I/R +L、I/R+D、blank화Sham조혈청내독소수평분별시(0.039 4±0.0025)、(0.0299±0.0039)、(0.028 9±0.003 1)、(0.0252±0.0042)화(0.026 2±0.001 3)Eu/ml;세포인자분별시종류배사인자-α(TNF-α):(49.92±7.48)、(36.85±13.46)、(27.67±10.12)、(18.86±12.70)화(25.80±11.19) ng/L;백세포개소(IL)-6:(41.78 ±9.75)、(19.14±1.48)、(19.20 ±4.14)、(8.69±0.76)화(11.57±2.90) ng/Lo I/R、I/R+L、I/R +D조현저고우blank조화Sham조(P＜0.05);조단림파통로후,I/R+L조화I/R+D조혈중적내독소화세포인자농도명현저우I/R조(P＜0.05);I/R+L、I/R +D조적일양화담(NO)、총일양화담합매(tNOS)화유도형일양화담합매(iNOS)농도여blank조화Sham조비교현저증고(P＜0.05);I/R+L、I/R+D조적NO화I/R+D조적iNOS농도현저저우I/R조(P＜0.05).결론 장림파관결찰혹림파액인류조단“장-림파”도경능감경장도결혈재관주도치적국부염성반응화원격조직적손상,림파액인류략우우림파관결찰,단차이무통계학의의.
Objective To compare the effects of using different ways to block "gut-lymph " pathway on endotoxin,cytokine,and lung function.Methods Fifty special pathogen free (SPF) leveled SD mice were randomly divided into 5 groups:control group (blank) ; sham group (sham) ; ischemia/reperfusion group (I/R) ; ischemia/reperfusion plus lymph duct ligation group (I/R + L) ; and ischemia/reperfusion plus lymph drainage group(I/R+D) (n=10).Results I/R,I/R+L and I/R + D groups had significantly higher levels of endotoxin and cytokine in serum than in blank group and sham group [endotoxin in I/R vs.I/R + L vs.I/R + D vs.blank vs.sham:(0.039 4 ±0.002 5) vs.(0.029 9 ±0.003 9) vs.(0.028 9 ±0.003 1) vs.(0.025 2 ±0.004 2) vs.(0.026 2 ±0.001 3) Eu/ml; tumor necrosis factor-α(TNF-α):(49.92±7.48) vs.(36.85 ± 13.46) vs.(27.67 ± 10.12) vs.(18.86 ± 12.70) vs.(25.80±11.19) ng/L; interleukin (IL)-6:(41.78±9.75) vs.(19.14±1.48) vs.(19.20±4.14)vs.(8.69 ± 0.76) vs.(11.57 ± 2.90) ng/L;P ＜ 0.05 respectively).After blocking lymph duct,I/R +L and I/R + D groups had remarkably lower levels of endotoxin and cytokine in serum,and nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in the lung than in I/R group (P ＜0.05),The concentrations of NO,total nitric oxide synthase (tNOS),and iNOS in I/R + L and I/R + D groups were extraordinarily higher than in sham group (P ＜ 0.05).Conclusion By blocking " gut-lymph" pathway,both lymph duct ligation and lymph drainage could reduce the local intestinal tract inflammation and distant organ injury incurred by intestinal ischemia/reperfusion,and the effect of lymph drainage was superior to lymph duct ligation,but with no significant difference.