CAJ | 학술논문

目的观察微小RNA(miRNA)-374a对乳腺癌细胞增殖、迁移能力的作用,以及对下游基因单核细胞趋化蛋白-1(MCP-1)的调控作用.方法利用质粒构建miRNA-374a过表达和空白对照细胞株;采用逆转录-聚合酶链反应(RT-PCR)方法检测374a-PLL3.7-MDA和EV-PLL3.7-MDA中miRNA-374a的表达,并分析其与乳腺癌细胞增殖、转移、迁移的相关性.结果细胞计数试剂盒(CCK-8)细胞增殖实验和单克隆形成实验结果显示,374a-PLL3.7-MDA组细胞增殖能力明显低于对照组细胞EV-PLL3.7-MDA.Transwell小室实验结果显示,374a-PLL3.7-MDA细胞小室膜下表面的细胞数明显少于对照组细胞.374a-PLL3.7-MDA组细胞中MCP-1的表达明显降低.结论 miRNA-374b可能通过MCP-1抑制乳腺癌细胞增殖、迁移能力.
목적 관찰미소RNA(miRNA)-374a대유선암세포증식、천이능력적작용,이급대하유기인단핵세포추화단백-1(MCP-1)적조공작용.방법 이용질립구건miRNA-374a과표체화공백대조세포주;채용역전록-취합매련반응(RT-PCR)방법검측374a-PLL3.7-MDA화EV-PLL3.7-MDA중miRNA-374a적표체,병분석기여유선암세포증식、전이、천이적상관성.결과 세포계수시제합(CCK-8)세포증식실험화단극륭형성실험결과현시,374a-PLL3.7-MDA조세포증식능력명현저우대조조세포EV-PLL3.7-MDA.Transwell소실실험결과현시,374a-PLL3.7-MDA세포소실막하표면적세포수명현소우대조조세포.374a-PLL3.7-MDA조세포중MCP-1적표체명현강저.결론 miRNA-374b가능통과MCP-1억제유선암세포증식、천이능력.
Objective To study the effect of microRNA (miRNA)-374a on proliferation and migration of breast cancer cells and regulation of monocyte chemoattractant protein-1 (MCP-1).Methods Construct miRNA-374a over-expression MDA-MB-231 breast cancer cells,374a-PLL3.7-MDAs and noload plasmid MDA-MB-231 breast cancer cells,EV-PLL3.7-MDAs by slow virus plasmid PLL3.7; Detect the expression of miRNA-374a by reverse transcriptase-polymerase chain reaction (RT-PCR).Results miRNA-374a can significantly inhibit breast cancer cell proliferation,migration ability.Cell counting kit-8 (CCK-8) cells proliferation assays and monoclonal formed assays show that 374a-PLL3.7-MDAs proliferation was inhibited than EV-PLL3.7-MDA.Transwell chambers Experimental results show that the chamber film surface of 374a-PLL3.7-MDAs was significantly less than EV-PLL3.7-MDAs.Expression of MCP-1 was inhibited in 374a-PLL3.7-MDAs.Conclusion miRNA-374b may inhibit proliferation and migration of breast cancer cells by MCP-1.