中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
2期
304-307
,共4页
鲁育民%袁晟光%梁科伟%孙彦浩%廖维甲%何松青
魯育民%袁晟光%樑科偉%孫彥浩%廖維甲%何鬆青
로육민%원성광%량과위%손언호%료유갑%하송청
胆固醇7α-羟化酶%胆汁酸%法尼酯衍生物X受体%肝再生
膽固醇7α-羥化酶%膽汁痠%法尼酯衍生物X受體%肝再生
담고순7α-간화매%담즙산%법니지연생물X수체%간재생
Cholesterol 7α-hydroxylase%Bile acid%Farnesoid X receptor%Liver regeneration
目的 探讨法尼酯衍生物X受体(FXR)与不同引流方式下梗阻性黄疸(OJ)大鼠部分肝切除术(PH)后肝再生差异的关系.方法 120只健康雄性大鼠随机分为4组:正常大鼠70％肝切除组(Control组)、梗阻性黄疸未引流切除组(OJ组)、梗阻性黄疸内引流切除组(ID组)、梗阻性黄疸外引流切除组(ED组).术后0、4、12、24、48、72 h等时间点检测大鼠血清总胆汁酸(TBA)含量,残肝组织FXR mRNA和蛋白表达、胆固醇7α-羟化酶(CYP7A1) mRNA表达.结果 ED组PH后12～72 h各时点增殖细胞核抗原(PCNA)阳性表达率为(19.52±1.87)％、(30.16 ±2.65)％、(46.44±2.66)％、(44.36±3.82)％,均明显低于ID组和Control组(P ＜0.05);ED组TBA含量各时点分别为(20.38 ±4.33)、(25.87±8.68)、(35.05±6.87)、(49.66±6.65)、(60.44±7.66)、(52.36±8.82) μmol/L,均低于Control组和ID组(P＜0.05);CYP7A1 mRNA相对表达量ED组各时段分别为1.75±0.09、1.68±0.14、1.53±0.16、1.25±0.16、1.17±0.18、1.24±0.16,均高于Control组和ID组(P＜0.05),ID组各时点FXR mRNA相对表达量分别为1.52±0.18、1.80 ±0.12、1.89±0.12、2.56±0.09、2.39±0.20、2.02±0.24,均高于ED组(P＜0.05),FXR蛋白表达与FXR mRNA基本一致;各项指标ID组与Control组各时点差异无统计学意义(P＞0.05).结论 经外引流的梗阻性黄疸大鼠部分肝切除后肝再生较内引流明显抑制的原因与胆酸丢失有关,可能机制为低表达的FXR抑制了肝再生.
目的 探討法尼酯衍生物X受體(FXR)與不同引流方式下梗阻性黃疸(OJ)大鼠部分肝切除術(PH)後肝再生差異的關繫.方法 120隻健康雄性大鼠隨機分為4組:正常大鼠70％肝切除組(Control組)、梗阻性黃疸未引流切除組(OJ組)、梗阻性黃疸內引流切除組(ID組)、梗阻性黃疸外引流切除組(ED組).術後0、4、12、24、48、72 h等時間點檢測大鼠血清總膽汁痠(TBA)含量,殘肝組織FXR mRNA和蛋白錶達、膽固醇7α-羥化酶(CYP7A1) mRNA錶達.結果 ED組PH後12～72 h各時點增殖細胞覈抗原(PCNA)暘性錶達率為(19.52±1.87)％、(30.16 ±2.65)％、(46.44±2.66)％、(44.36±3.82)％,均明顯低于ID組和Control組(P ＜0.05);ED組TBA含量各時點分彆為(20.38 ±4.33)、(25.87±8.68)、(35.05±6.87)、(49.66±6.65)、(60.44±7.66)、(52.36±8.82) μmol/L,均低于Control組和ID組(P＜0.05);CYP7A1 mRNA相對錶達量ED組各時段分彆為1.75±0.09、1.68±0.14、1.53±0.16、1.25±0.16、1.17±0.18、1.24±0.16,均高于Control組和ID組(P＜0.05),ID組各時點FXR mRNA相對錶達量分彆為1.52±0.18、1.80 ±0.12、1.89±0.12、2.56±0.09、2.39±0.20、2.02±0.24,均高于ED組(P＜0.05),FXR蛋白錶達與FXR mRNA基本一緻;各項指標ID組與Control組各時點差異無統計學意義(P＞0.05).結論 經外引流的梗阻性黃疸大鼠部分肝切除後肝再生較內引流明顯抑製的原因與膽痠丟失有關,可能機製為低錶達的FXR抑製瞭肝再生.
목적 탐토법니지연생물X수체(FXR)여불동인류방식하경조성황달(OJ)대서부분간절제술(PH)후간재생차이적관계.방법 120지건강웅성대서수궤분위4조:정상대서70％간절제조(Control조)、경조성황달미인류절제조(OJ조)、경조성황달내인류절제조(ID조)、경조성황달외인류절제조(ED조).술후0、4、12、24、48、72 h등시간점검측대서혈청총담즙산(TBA)함량,잔간조직FXR mRNA화단백표체、담고순7α-간화매(CYP7A1) mRNA표체.결과 ED조PH후12～72 h각시점증식세포핵항원(PCNA)양성표체솔위(19.52±1.87)％、(30.16 ±2.65)％、(46.44±2.66)％、(44.36±3.82)％,균명현저우ID조화Control조(P ＜0.05);ED조TBA함량각시점분별위(20.38 ±4.33)、(25.87±8.68)、(35.05±6.87)、(49.66±6.65)、(60.44±7.66)、(52.36±8.82) μmol/L,균저우Control조화ID조(P＜0.05);CYP7A1 mRNA상대표체량ED조각시단분별위1.75±0.09、1.68±0.14、1.53±0.16、1.25±0.16、1.17±0.18、1.24±0.16,균고우Control조화ID조(P＜0.05),ID조각시점FXR mRNA상대표체량분별위1.52±0.18、1.80 ±0.12、1.89±0.12、2.56±0.09、2.39±0.20、2.02±0.24,균고우ED조(P＜0.05),FXR단백표체여FXR mRNA기본일치;각항지표ID조여Control조각시점차이무통계학의의(P＞0.05).결론 경외인류적경조성황달대서부분간절제후간재생교내인류명현억제적원인여담산주실유관,가능궤제위저표체적FXR억제료간재생.
Objective To evaluate the relationship between farnesoid X receptor (FXR) and liver regeneration of obstructive jaundice (OJ) rats following partial hepatectomy (PH) with different biliary drainages.Methods 120 male Sprague-Dawley rats were randomly allocated into 4 groups:control group,normal rats subjected to PH (70％ hepatectomy) ; OJ group,OJ rats without drainage subjected to PH; internal drainage (ID) group,OJ rats with ID subjected to PH; external drainage (ED) group,OJ rats with ED subjected to PH.The expression of FXR mRNA and protein and cholesterol 7α-hydroxylase (CYP7A1)mRNA in liver tissue,and total bile acid (TBA) content in the blood were examined at0,4,12,24,48,72 h after operation.Results The positive rate of proliferating cell nuclear antigen (PCNA) [(19.52 ±1.87)％,(30.16 ±2.65)％,(46.44 ±2.66)％,(44.36 ±3.82)％] during 12 to 72 h after PH in ED group was obviously lower than that in control and ID groups (P ＜ 0.05).The TBA content in ED group [(20.38±4.33),(25.87 ±8.68),(35.05 ±6.87),(49.66±6.65),(60.44±7.66),(52.36±8.82) μmol/L] during 0 to 72 h after PH was obviously lower than that in control and ID groups (P＜0.05).The relative quantitative expression of CYP7A1 mRNA in ED group (1.75 ±0.09,1.68 ±0.14,1.53 ±0.16,1.25 ±0.16,1.17 ±0.18,1.24 ±0.16) during 0 to 72 h after PH was obviously higher than that in control and ID groups (P ＜ 0.05).The relative quantitative expression of FXR mRNA in ID group [(1.52±0.18),(1.80±0.12),(1.89±0.12),(2.56 ±0.09),(2.39 ±0.20),(2.02 ±0.24)] during 0 to 72 h after PH was obviously higher than that in ED group (P ＜ 0.05).The expression of FXR protein was corresponded with FXR mRNA generally.There was no statistically significant difference between ID and control groups in each index (P ＞ 0.05).Conclusion The inhibition of the liver regeneration in the OJ rats after PH with ED versus ID is related with the reduction in the bile acid pool size,which may be caused by the down-regulation of FXR expression.
