中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
2期
312-315
,共4页
蒙家兴%潘运龙%赵晓旭%丁晖%覃莉
矇傢興%潘運龍%趙曉旭%丁暉%覃莉
몽가흥%반운룡%조효욱%정휘%담리
癌,肝细胞%纳米金%血管内皮生长因子%腹水生成
癌,肝細胞%納米金%血管內皮生長因子%腹水生成
암,간세포%납미금%혈관내피생장인자%복수생성
Carcinoma,hepatocellular%Gold nanoparticles%Vascular endothelial growth factor%Ascites accumulation
目的 观察纳米金(AuNP)对HepG2细胞血管内皮生长因子(VEGF)表达和分泌的影响.方法 实验分为不同浓度AuNP(5、10、20 μg/L)处理组和对照组,HepG2细胞常规消化种板,培养24h后:AuNP处理组分别加入浓度为5、10、20 μg/L的AuNP溶液1 ml;对照组加入等量无血清培养液,继续培养12h后:采用Western blot法检测HepG2细胞内VEGF的表达水平;酶联免疫吸附试验(ELISA)检测HepG2细胞VEGF的分泌水平;噻唑蓝(MTT)比色法检测HepG2细胞增殖率.改变HepG2细胞的培养条件(37℃/4℃),观察AuNP对HepG2细胞VEGF分泌的影响.建立肝癌腹水瘤模型进一步观察AuNP对模型小鼠腹水VEGF水平及腹水量的影响.结果 体外实验:各组HepG2细胞分泌的VEGF浓度分别为:对照组(351.64±7.89)ng/L;5μg/L AuNP组(285.62±3.45) ng/L;10 μg/L AuNP组(121.72±3.10) ng/L;20 μg/L AuNP组(9.83±2.28) ng/L,P＜0.05.各AuNP处理组(5、10、20 μg/L) HepG2细胞增殖率分别为:(98.98±7.57)％、(91.09±11.46)％、(92.13±5.65)％,各组AuNP对HepG2细胞增殖影响差异均无统计学意义(P＞0.05).体内实验:腹水瘤模型小鼠腹水VEGF水平及腹水体积分别为:对照组(62.95±11.93) ng/L、(13.22±1.03) ml;AuNP处理组(27.12 ±8.58) ng/L、(5.21 ±0.62) ml,P＜0.01.结论 AuNP可以明显抑制HepG2细胞VEGF的表达和分泌,体内实验证实AuNP能够降低腹水瘤模型小鼠腹水VEGF水平,明显减少腹水量.
目的 觀察納米金(AuNP)對HepG2細胞血管內皮生長因子(VEGF)錶達和分泌的影響.方法 實驗分為不同濃度AuNP(5、10、20 μg/L)處理組和對照組,HepG2細胞常規消化種闆,培養24h後:AuNP處理組分彆加入濃度為5、10、20 μg/L的AuNP溶液1 ml;對照組加入等量無血清培養液,繼續培養12h後:採用Western blot法檢測HepG2細胞內VEGF的錶達水平;酶聯免疫吸附試驗(ELISA)檢測HepG2細胞VEGF的分泌水平;噻唑藍(MTT)比色法檢測HepG2細胞增殖率.改變HepG2細胞的培養條件(37℃/4℃),觀察AuNP對HepG2細胞VEGF分泌的影響.建立肝癌腹水瘤模型進一步觀察AuNP對模型小鼠腹水VEGF水平及腹水量的影響.結果 體外實驗:各組HepG2細胞分泌的VEGF濃度分彆為:對照組(351.64±7.89)ng/L;5μg/L AuNP組(285.62±3.45) ng/L;10 μg/L AuNP組(121.72±3.10) ng/L;20 μg/L AuNP組(9.83±2.28) ng/L,P＜0.05.各AuNP處理組(5、10、20 μg/L) HepG2細胞增殖率分彆為:(98.98±7.57)％、(91.09±11.46)％、(92.13±5.65)％,各組AuNP對HepG2細胞增殖影響差異均無統計學意義(P＞0.05).體內實驗:腹水瘤模型小鼠腹水VEGF水平及腹水體積分彆為:對照組(62.95±11.93) ng/L、(13.22±1.03) ml;AuNP處理組(27.12 ±8.58) ng/L、(5.21 ±0.62) ml,P＜0.01.結論 AuNP可以明顯抑製HepG2細胞VEGF的錶達和分泌,體內實驗證實AuNP能夠降低腹水瘤模型小鼠腹水VEGF水平,明顯減少腹水量.
목적 관찰납미금(AuNP)대HepG2세포혈관내피생장인자(VEGF)표체화분비적영향.방법 실험분위불동농도AuNP(5、10、20 μg/L)처리조화대조조,HepG2세포상규소화충판,배양24h후:AuNP처리조분별가입농도위5、10、20 μg/L적AuNP용액1 ml;대조조가입등량무혈청배양액,계속배양12h후:채용Western blot법검측HepG2세포내VEGF적표체수평;매련면역흡부시험(ELISA)검측HepG2세포VEGF적분비수평;새서람(MTT)비색법검측HepG2세포증식솔.개변HepG2세포적배양조건(37℃/4℃),관찰AuNP대HepG2세포VEGF분비적영향.건립간암복수류모형진일보관찰AuNP대모형소서복수VEGF수평급복수량적영향.결과 체외실험:각조HepG2세포분비적VEGF농도분별위:대조조(351.64±7.89)ng/L;5μg/L AuNP조(285.62±3.45) ng/L;10 μg/L AuNP조(121.72±3.10) ng/L;20 μg/L AuNP조(9.83±2.28) ng/L,P＜0.05.각AuNP처리조(5、10、20 μg/L) HepG2세포증식솔분별위:(98.98±7.57)％、(91.09±11.46)％、(92.13±5.65)％,각조AuNP대HepG2세포증식영향차이균무통계학의의(P＞0.05).체내실험:복수류모형소서복수VEGF수평급복수체적분별위:대조조(62.95±11.93) ng/L、(13.22±1.03) ml;AuNP처리조(27.12 ±8.58) ng/L、(5.21 ±0.62) ml,P＜0.01.결론 AuNP가이명현억제HepG2세포VEGF적표체화분비,체내실험증실AuNP능구강저복수류모형소서복수VEGF수평,명현감소복수량.
Objective To observe the inhibitory effect of gold nanoparticles on the expression and secretion of vascular endothelial growth factor (VEGF) by HepG2 cells.Methods HepG2 cells were seeded in 96-well plates and treated with different concentrations (5,10,and 20 μg/L) of gold nanoparticles for 12 h.The concentration of VEGF protein expressed and secreted by HepG2 cells was determined by using Western blotting and enzyme linked immunosorbent assay (ELISA) separately.The proliferation rate of HepG2 cells was assessed by using methyl thiazol tetrazolium (MTT) assay.For mouse ascites model experiments,H22 cells were intraperitoneally injected into BALB/c mice.After 7 days,gold nanoparticles (1 mg/kg) was administered through intraperitoneal injection every 2 days over a period of 14 days.Mice were then sacrificed and ascites fluids collected.The fluid accumulation in the peritoneal carvity,as well as the concentration of VEGF of mouse ascites was determined.Results The VEGF concentration of 4 groups (control,5,10 and 20 μg/L gold nanoparticles) was (351.64 ±7.89),(285.62 ± 3.45),(121.72 ±3.10) and (9.83 ±2.28) ng/L respectively,P ＜0.05.The proliferation rate of HepG2 cells in three gold nanoparticles group (5,10,and 20 μg/L) was (98.98 ±7.57)％,(90.95 ± 11.62)％ and (86.38 ±4.23)％ respectively,P ＞ 0.05.The ascites accumulation and VEGF concentration in control and gold nanoparticles groups were (13.22 ± 1.03) ml and (62.95 ± 11.93) ng/L,and (5.21 ± 0.62) ml and (27.12 ± 8.58) ng/L respectively,P ＜ 0.01.Conclusion Gold nanoparticles can inhibit the expression and secretion of VEGF and cause lesser ascites accumulation of mouse ascites model.
