CAJ | 학술논문

目的观察白噬在血管抑制药物贝伐单抗诱导的肺癌A549细胞凋亡中的作用.方法设置空白对照组、贝伐单抗(16μmol/L)组、3-甲基腺嘌呤(3-MA,8μmol/L)组和3-MA+贝伐单抗组4个实验组.收集各实验组细胞组,膜联蛋白Ⅴ-异硫氰酸荧光素(Annexin Ⅴ-FITC)和单丹磺酰戊二胺(MDC)染色后,荧光显微镜下定性观察荧光表达;流式细胞术定量检测细胞凋亡、Westernblot检测自噬相关蛋白Beclin 1和微管相关蛋白1轻链3(LC3)的表达.结果 (1)作用48 h,贝伐单抗对A549细胞的半数抑制浓度(IC50)为16 μmol/L,3-MA对A549细胞抑制率10％的浓度(IR10)为8μmol/L; (2)与单用贝伐单抗[(43.92±1.38)％]比较,3-MA联合贝伐单抗显著增加了细胞凋亡率[(87.46±5.97)％,P＜0.01],Beclin l和LC3的表达显著下调(P＜0.05).结论 3-MA可能是通过抑制白噬信号通路,显著增加了贝伐单抗诱导的肺癌细胞凋亡.
목적 관찰백서재혈관억제약물패벌단항유도적폐암A549세포조망중적작용.방법 설치공백대조조、패벌단항(16μmol/L)조、3-갑기선표령(3-MA,8μmol/L)조화3-MA+패벌단항조4개실험조.수집각실험조세포조,막련단백Ⅴ-이류청산형광소(Annexin Ⅴ-FITC)화단단광선무이알(MDC)염색후,형광현미경하정성관찰형광표체;류식세포술정량검측세포조망、Westernblot검측자서상관단백Beclin 1화미관상관단백1경련3(LC3)적표체.결과 (1)작용48 h,패벌단항대A549세포적반수억제농도(IC50)위16 μmol/L,3-MA대A549세포억제솔10％적농도(IR10)위8μmol/L; (2)여단용패벌단항[(43.92±1.38)％]비교,3-MA연합패벌단항현저증가료세포조망솔[(87.46±5.97)％,P＜0.01],Beclin l화LC3적표체현저하조(P＜0.05).결론 3-MA가능시통과억제백서신호통로,현저증가료패벌단항유도적폐암세포조망.
Objective To study the role of anti-proliferation effect of bevacizumab on autophagy inhibitor on non-small cell lung caner (NSCLC).Methods The lung cencer cells A549 cells were treated with bevacizumab (Beva,16 μmol/L) and/or 3-methyladenine (3-MA,8 μmol/L).Flow cytometry was performed to examine the cell apoptosis rate treated with annexin Ⅴ-fluoresceine isothiocyanate (FITC)/propidium iodide (PI) double staining; Cells stained by monodansylcadaverine (MDC) were observed qualitatively under a fluorescence microscope.Western blotting assay was used to investigate autophagy-related Beclin 1 and microtubule-associated protein 1 light chain 3 (LC3) changes that occurred in the course of treatment.Results The flow cytometry indicated that the respective apoptosis rate of Control,Beva (16 μ mol/L),3-MA (8 μmol/L) and Beva + 3-MA group were (3.45 ± 0.28) ％,(43.92 ± 1.38) ％,(7.63 ± 0.78) ％ and (87.46 ± 5.97) ％.The autophagic vacuoles in Beva group were obviously increased,but the phenomenon was inhibited after combination with 3-MA.The expression of Beclin 1 and LC3 was clearly up-regulated in Beva group,while the degree of expression in combinition group dec reased significantly when compared with treatment with Beva alone.Conclusion Beva can induce the apoptosis and increase the autophagy in A549 cells,which could be reduced after co-treated with 3-MA.