中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
2期
341-343
,共3页
廖洪映%蔡松旺%李昀%张健%黄邵洪%陈惠国%吴伟彬%李小军
廖洪映%蔡鬆旺%李昀%張健%黃邵洪%陳惠國%吳偉彬%李小軍
료홍영%채송왕%리윤%장건%황소홍%진혜국%오위빈%리소군
微小RNA-126%血管内皮生长因子%增殖%肺腺癌
微小RNA-126%血管內皮生長因子%增殖%肺腺癌
미소RNA-126%혈관내피생장인자%증식%폐선암
MicroRNA-126%Vascular endothelial growth factor%Proliferation%Lung adenocarcinoma
目的 观察体外转染微小RNA-126(miR-126)对人肺腺癌A549细胞增殖的影响.方法 人肺腺癌A549细胞株进行体外培养,分为3组:A组用单纯脂质体处理,B组用miR-126对照系列处理,C组用miR-126处理.实时定量聚合酶链反应(Real-time PCR)检测miR-126表达,Western blot检测血管内皮生长因子(VEGF)蛋白的表达,噻唑蓝(MTT)比色法检测A549细胞增殖.结果 转染人工合成的miR-126后的C组miR-126表达上调至(0.786±0.132),与A组(0.453±0.085)、B组(0.432±0.092)比较差异有统计学意义(P＜0.01);C组细胞的VEGF表达下调至(0.312±0.075),与A组(0.633±0.172)、B组(0.597±0.095)比较差异有统计学意义(P＜0.01);C组的细胞增殖被明显抑制,与对照组比较差异有统计学意义(P＜0.05).结论 转染miR-126能够明显增加A549细胞内miR-126的表达,并下调VEGF蛋白表达,抑制肺腺癌A549细胞生长.
目的 觀察體外轉染微小RNA-126(miR-126)對人肺腺癌A549細胞增殖的影響.方法 人肺腺癌A549細胞株進行體外培養,分為3組:A組用單純脂質體處理,B組用miR-126對照繫列處理,C組用miR-126處理.實時定量聚閤酶鏈反應(Real-time PCR)檢測miR-126錶達,Western blot檢測血管內皮生長因子(VEGF)蛋白的錶達,噻唑藍(MTT)比色法檢測A549細胞增殖.結果 轉染人工閤成的miR-126後的C組miR-126錶達上調至(0.786±0.132),與A組(0.453±0.085)、B組(0.432±0.092)比較差異有統計學意義(P＜0.01);C組細胞的VEGF錶達下調至(0.312±0.075),與A組(0.633±0.172)、B組(0.597±0.095)比較差異有統計學意義(P＜0.01);C組的細胞增殖被明顯抑製,與對照組比較差異有統計學意義(P＜0.05).結論 轉染miR-126能夠明顯增加A549細胞內miR-126的錶達,併下調VEGF蛋白錶達,抑製肺腺癌A549細胞生長.
목적 관찰체외전염미소RNA-126(miR-126)대인폐선암A549세포증식적영향.방법 인폐선암A549세포주진행체외배양,분위3조:A조용단순지질체처리,B조용miR-126대조계렬처리,C조용miR-126처리.실시정량취합매련반응(Real-time PCR)검측miR-126표체,Western blot검측혈관내피생장인자(VEGF)단백적표체,새서람(MTT)비색법검측A549세포증식.결과 전염인공합성적miR-126후적C조miR-126표체상조지(0.786±0.132),여A조(0.453±0.085)、B조(0.432±0.092)비교차이유통계학의의(P＜0.01);C조세포적VEGF표체하조지(0.312±0.075),여A조(0.633±0.172)、B조(0.597±0.095)비교차이유통계학의의(P＜0.01);C조적세포증식피명현억제,여대조조비교차이유통계학의의(P＜0.05).결론 전염miR-126능구명현증가A549세포내miR-126적표체,병하조VEGF단백표체,억제폐선암A549세포생장.
Objective To explore the effect of microRNA-126 (miR-126) on the proliferation of lung adenocarcinoma A549 cells.Methods 549 cells in vitro were divided into 3 groups:group A treated only with liposomes,group B with miR-126 control,and group C with miR-126.The expression of miR-126 was detected by using Real-time quantitative polymerase chain reaction (Real-time PCR),and that of vascular endothelial growth factor (VEGF) protein by Western blotting,and methyl thiazol tetrazolium (MTr) assay was used to examine the growth of A549 cells.Results In group C,the expression level of miR-126 was increased to (0.786 ±0.132) as compared with group A (0.453 ±0.085) and group B (0.432 ±0.092) (P ＜0.01),and the VEGF protein expression was decreased to (0.312 ± 0.075) as compared with group A (0.633 ±0.172) and group B (0.597 ±0.095) (P ＜0.01).The growth of A549 cells in group C was inhibited significantly as compared with group A and group B (P ＜ 0.05).Conclusion MiR-126 transfection can significantly increase its expression in A549 cells,decrease the expression of VEGF protein,and suppress the cells growth.
