CAJ | 학술논문

目的设计一种生物活性玻璃(BG)/壳聚糖(CS)复合材料的骨组织工程支架,检测其理化性能和细胞相容性.方法 2.0％ CS盐酸溶液与β-甘油磷酸钠在冰浴条件下以7∶1比例混合搅拌均匀.电子天平分别称取BG分别加入上述CS溶液中,分别制成理论质量比为2.0∶1.0、1.0∶1.0、1.0∶1.5的CS/BG混合液.冷冻干燥成型,20 kGy60Go辐射灭菌后得到样品.通过扫描电镜、X线衍射和傅立叶红外光谱分析、DSC热分析其微观形貌和组成;计算支架孔孔隙率;采用陶瓷试验系统进行支架的机械性能检测并评价BG的加入对支架的影响.将第3代兔骨髓间充质干细胞接种于支架上,使用扫描电镜检测支架对其黏附作用,采用噻唑蓝(MTT)法检测细胞在支架上增殖,并评价支架的细胞相容性.结果合成的CS/BG支架与模具拥有同样的大小及几何形状,具有相互贯通的多孔结构,未见BG聚集,孔隙率最高可达89％,孔径大小合适(100 ～ 300μm),最大断裂强度为(4.90±0.63) mPa.X射线衍射图可以看到特征性的BG衍射峰;傅立叶变换红外光谱可见特征的BG吸收峰,这表明材料内有明确的BG;第3代兔骨髓间充质干细胞在支架上共培养ld后,细胞在支架表面黏附,部分细胞伸展,并伸出伪足.共培养7d后,可见细胞生长良好,沿材料铺展生长,细胞呈多角形,细胞伸出多个细长的伪足与材料相连.采用MTT法测量骨髓间充质干细胞在支架上的增殖,结果表明骨髓间充质干细胞在CS/BG支架上表现出了明显的增殖.结论采用溶液共混、冷冻干燥法可以制备出CS/BG支架;支架具有良好的孔隙率、较好的机械强度、良好的组织相容性,可用于骨组织工程.
목적 설계일충생물활성파리(BG)/각취당(CS)복합재료적골조직공정지가,검측기이화성능화세포상용성.방법 2.0％ CS염산용액여β-감유린산납재빙욕조건하이7∶1비례혼합교반균균.전자천평분별칭취BG분별가입상술CS용액중,분별제성이론질량비위2.0∶1.0、1.0∶1.0、1.0∶1.5적CS/BG혼합액.냉동간조성형,20 kGy60Go복사멸균후득도양품.통과소묘전경、X선연사화부립협홍외광보분석、DSC열분석기미관형모화조성;계산지가공공극솔;채용도자시험계통진행지가적궤계성능검측병평개BG적가입대지가적영향.장제3대토골수간충질간세포접충우지가상,사용소묘전경검측지가대기점부작용,채용새서람(MTT)법검측세포재지가상증식,병평개지가적세포상용성.결과 합성적CS/BG지가여모구옹유동양적대소급궤하형상,구유상호관통적다공결구,미견BG취집,공극솔최고가체89％,공경대소합괄(100 ～ 300μm),최대단렬강도위(4.90±0.63) mPa.X사선연사도가이간도특정성적BG연사봉;부립협변환홍외광보가견특정적BG흡수봉,저표명재료내유명학적BG;제3대토골수간충질간세포재지가상공배양ld후,세포재지가표면점부,부분세포신전,병신출위족.공배양7d후,가견세포생장량호,연재료포전생장,세포정다각형,세포신출다개세장적위족여재료상련.채용MTT법측량골수간충질간세포재지가상적증식,결과표명골수간충질간세포재CS/BG지가상표현출료명현적증식.결론 채용용액공혼、냉동간조법가이제비출CS/BG지가;지가구유량호적공극솔、교호적궤계강도、량호적조직상용성,가용우골조직공정.
Objective To design a kind of bioactive glasses/chitosan (BG/CS) composite materials,and investigate their physico-chemical properties and cell compatibility.Methods 2.0％ chitosan of hydrochloric acid solution and β-glycerophosphate on ice conditions in the 7∶1 ratio of well mixed.after CS/BG become 2.0∶ 1.0,1.0∶ 1.0,1.0∶1.5 gelatinous liquid stage.Save it in the-80 ℃ frige,formed by freeze-drying machine.After 20 kGy 60Go radiation sterilization get the sample.By scanning electron microscope,X-ray diffraction and Fourier infrared spectral analysis,DSC thermal analysis,its microstructure and composition,Calculate the stents' porosity,adopt ceramic test system of mechanical properties test.Inoculate the third-generation rabbit mesenchymal stem cells into the stents and use electron microscopy scanning the adherency of stents.Detect the proliferation of cells which are adhere to the stents.Evaluate the compatibility of the stents and cells.Results Synthetic CS/BG stents has the same size and geometric shapes with mould.The phenomenon of hydroxyapatite crystals gathered and carbon nanotube reunion was not detected.The porosity is 81％-89％,it is appropriate.The breaking strength is (4.90 ±0.63) mPa,Characteristic BG diffraction peak can be seen through X-ray diffraction figure technology.The absorption peaks of BG can be detected through fourier transform infrared spectroscopy.It indicate that BG is consist in the material.The third generation of rabbit rabbit mesenchymal stem cells adhere to the stent when cultivate for one day,some cells extends and put out locomotions.Training 7 days later,the cells grows well,and spread along the stent,it can be seen that cells polygonal and cell with material connected through slender locomotion.bone marrow mesenchymal stem cell grows well in the CS/BG stents.Conclusion The CS/BG stents can be preparate through the solution blending and freeze drying technology.The stents has good porosity,high mechanical strength and good compatibility with cells.It can be used for organization of bone tissue engineering.