CAJ | 학술논문

目的观察微小RNA(miR)-29a对人胃癌SGC-7901细胞裸鼠皮下移植瘤生长的影响.方法以磷酸盐缓冲液(PBS)为空白对照,以负载cel-miR67的复制缺陷型腺病毒(Ad-cel)为阴性对照,注射负载miR-29a的复制缺陷型腺病毒(Ad-miR29a)液于移植瘤内,观察移植瘤生长曲线和抑瘤率;采用实时定量逆转录聚合酶链反应(RT-qPCR)检测移植瘤内miR-29a表达丰度;采用免疫组织化学技术检测CD34表达以计算移植瘤内微血管密度(MVD).结果与PBS组比较,Ad-miR29a组移植瘤内miR-29a表达显著上调(3.06±0.73比1.00±0.21,P＜0.01),而Ad-cel组与PBS组比较差异无统计学意义(P＞0.05);Ad-miR29a组移植瘤生长速度低于PBS组,而Ad-cel组与PBS组差异无统计学意义(P＞0.05);于第5周末Ad-miR29a组抑瘤率达49.30％;Ad-miR29a组的移植瘤内MVD显著低于PBS组(21.3±8.1比42.6±11.2,P＜0.01),而Ad-cel组与PBS组比较差异无统计学意义(P＞0.05).结论 miR-29a可显著抑制裸鼠胃癌移植瘤生长,其机制可能为抑制移植瘤内MVD,miR-29a有望成为新型的胃癌基因治疗靶标.
목적 관찰미소RNA(miR)-29a대인위암SGC-7901세포라서피하이식류생장적영향.방법 이린산염완충액(PBS)위공백대조,이부재cel-miR67적복제결함형선병독(Ad-cel)위음성대조,주사부재miR-29a적복제결함형선병독(Ad-miR29a)액우이식류내,관찰이식류생장곡선화억류솔;채용실시정량역전록취합매련반응(RT-qPCR)검측이식류내miR-29a표체봉도;채용면역조직화학기술검측CD34표체이계산이식류내미혈관밀도(MVD).결과 여PBS조비교,Ad-miR29a조이식류내miR-29a표체현저상조(3.06±0.73비1.00±0.21,P＜0.01),이Ad-cel조여PBS조비교차이무통계학의의(P＞0.05);Ad-miR29a조이식류생장속도저우PBS조,이Ad-cel조여PBS조차이무통계학의의(P＞0.05);우제5주말Ad-miR29a조억류솔체49.30％;Ad-miR29a조적이식류내MVD현저저우PBS조(21.3±8.1비42.6±11.2,P＜0.01),이Ad-cel조여PBS조비교차이무통계학의의(P＞0.05).결론 miR-29a가현저억제라서위암이식류생장,기궤제가능위억제이식류내MVD,miR-29a유망성위신형적위암기인치료파표.
Objective To study the effect of microRNA (miR)-29a on the growth of human gastric cancer cell line SGC-7901 xenografts in nude mice.Methods The SGC-7901 cell line was injected subcutaneously into nude mice to establish the model of human gastric cancer xenografts.The replicationdeficient recombinant adenovirus carrying miR-29a (Ad-miR29a) was injected into the xenografts.The phosphate buffered saline (PBS) solution was employed as blank control and the adenovirus carrying celmiR-67 (Ad-cel) as negative control.The growth curve of the tumor xenografts was observed and the inhibitory rate of tumor was calculated.The expression of miR-29a was detected using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR).The microvessel density (MVD) was calculated by CD34 detection using immunohistochemistry in the xenografts.Results Comparing to the PBS group,the expression level of miR-29a of xenografts in the Ad-miR29a group was significantly up-regulated (3.06 ± 0.73 vs.1.00 ±0.21,P ＜ 0.01),while no significant difference was detected between Ad-cel and PBS group.The growth of xenografts in the Ad-miR29a group was suppressed apparently comparing to the PBS group,while no significant change was detected between Ad-cel and PBS group.At the end of the 5th weekend,the tumor inhibition rate reached 49.30％ in Ad-miR29a group.The MVD counts of xenografts in the Ad-miR29a group were down-regulated significantly comparing to that in PBS group (21.3 ± 8.1 vs.42.6 ± 11.2,P ＜0.01),while no significant change was detected between Ad-cel and PBS group.Conclusion The up-regulation of miR-29a can suppress the growth of gastric cancer xenografts in nude mice by inhibiting the MVD in tumors,which makes miR-29a a promising novel target for gene therapy for human gastric cancer.