中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
3期
511-513
,共3页
冯洁%袁燕玲%刘爱群%葛莲英
馮潔%袁燕玲%劉愛群%葛蓮英
풍길%원연령%류애군%갈련영
胃肿瘤%Fas相关因子1%脱噬作用%幽门螺杆菌
胃腫瘤%Fas相關因子1%脫噬作用%幽門螺桿菌
위종류%Fas상관인자1%탈서작용%유문라간균
Stomach neoplasms%Fas-associated factor 1%Apoptosis%Helicobacter pylori
目的 探讨Fas相关因子l(FAF1)基因表达与幽门螺杆菌感染(Hp)及胃癌发生之间的关系.方法 人胃癌细胞株HGC-27、人胃黏膜上皮细胞GES-1 8×105个与Hp标准菌株NCTC11637共培养24h,按细胞/细菌比分为3组:空白对照组、1∶100和1∶200共培养组,每组实验设3个复孔,采用噻唑蓝(MTT)比色法490 nm波长处测量吸光度值计算细胞存活率,流式细胞仪检测l×104个细胞凋亡率,荧光实时定量聚合酶链反应(FQ-PCR)检测细胞FAFl mRNA相对表达量,扩增片段长度为164 bp.结果 HGC-27和GES-1细胞与Hp共培养后,细胞增殖均减少,凋亡率增加.与各自空白对照组比较,FAFl mRNA在HGC-27细胞1∶100和1∶200共培养组表达量明显降低(0.67 ±0.20比0.99 ±0.27,F=11.51,P＜0.05;0.44 ±0.30比0.99±0.27,F=11.51,P＜0.01);FAFl mRNA在GES-1细胞1∶100共培养组表达量明显升高(1.61 ±0.77比0.99 ±0.41,F=14.45,P＜0.05),1∶200共培养组表达量明显降低(0.36 ±0.22比0.99±0.41,F=14.45,P＜0.05).与GES-1细胞空白对照组、1∶100和1∶200共培养组比较,FAF1 mRNA在HGC-27相应各组表达量均明显降低(0.33 ±0.16比0.99 ±0.41,t =4.74,P＜0.01;0.17±0.12比1.08 ±0.53,t=5.28,P＜0.01;0.39 ±0.24比0.98±0.23,t=5.67,P＜0.01).结论 Hp感染下调FAF1基因表达,导致细胞增殖和凋亡失衡,诱发胃癌的发生.
目的 探討Fas相關因子l(FAF1)基因錶達與幽門螺桿菌感染(Hp)及胃癌髮生之間的關繫.方法 人胃癌細胞株HGC-27、人胃黏膜上皮細胞GES-1 8×105箇與Hp標準菌株NCTC11637共培養24h,按細胞/細菌比分為3組:空白對照組、1∶100和1∶200共培養組,每組實驗設3箇複孔,採用噻唑藍(MTT)比色法490 nm波長處測量吸光度值計算細胞存活率,流式細胞儀檢測l×104箇細胞凋亡率,熒光實時定量聚閤酶鏈反應(FQ-PCR)檢測細胞FAFl mRNA相對錶達量,擴增片段長度為164 bp.結果 HGC-27和GES-1細胞與Hp共培養後,細胞增殖均減少,凋亡率增加.與各自空白對照組比較,FAFl mRNA在HGC-27細胞1∶100和1∶200共培養組錶達量明顯降低(0.67 ±0.20比0.99 ±0.27,F=11.51,P＜0.05;0.44 ±0.30比0.99±0.27,F=11.51,P＜0.01);FAFl mRNA在GES-1細胞1∶100共培養組錶達量明顯升高(1.61 ±0.77比0.99 ±0.41,F=14.45,P＜0.05),1∶200共培養組錶達量明顯降低(0.36 ±0.22比0.99±0.41,F=14.45,P＜0.05).與GES-1細胞空白對照組、1∶100和1∶200共培養組比較,FAF1 mRNA在HGC-27相應各組錶達量均明顯降低(0.33 ±0.16比0.99 ±0.41,t =4.74,P＜0.01;0.17±0.12比1.08 ±0.53,t=5.28,P＜0.01;0.39 ±0.24比0.98±0.23,t=5.67,P＜0.01).結論 Hp感染下調FAF1基因錶達,導緻細胞增殖和凋亡失衡,誘髮胃癌的髮生.
목적 탐토Fas상관인자l(FAF1)기인표체여유문라간균감염(Hp)급위암발생지간적관계.방법 인위암세포주HGC-27、인위점막상피세포GES-1 8×105개여Hp표준균주NCTC11637공배양24h,안세포/세균비분위3조:공백대조조、1∶100화1∶200공배양조,매조실험설3개복공,채용새서람(MTT)비색법490 nm파장처측량흡광도치계산세포존활솔,류식세포의검측l×104개세포조망솔,형광실시정량취합매련반응(FQ-PCR)검측세포FAFl mRNA상대표체량,확증편단장도위164 bp.결과 HGC-27화GES-1세포여Hp공배양후,세포증식균감소,조망솔증가.여각자공백대조조비교,FAFl mRNA재HGC-27세포1∶100화1∶200공배양조표체량명현강저(0.67 ±0.20비0.99 ±0.27,F=11.51,P＜0.05;0.44 ±0.30비0.99±0.27,F=11.51,P＜0.01);FAFl mRNA재GES-1세포1∶100공배양조표체량명현승고(1.61 ±0.77비0.99 ±0.41,F=14.45,P＜0.05),1∶200공배양조표체량명현강저(0.36 ±0.22비0.99±0.41,F=14.45,P＜0.05).여GES-1세포공백대조조、1∶100화1∶200공배양조비교,FAF1 mRNA재HGC-27상응각조표체량균명현강저(0.33 ±0.16비0.99 ±0.41,t =4.74,P＜0.01;0.17±0.12비1.08 ±0.53,t=5.28,P＜0.01;0.39 ±0.24비0.98±0.23,t=5.67,P＜0.01).결론 Hp감염하조FAF1기인표체,도치세포증식화조망실형,유발위암적발생.
Objective To investigate the relationship between the Fas-associated factor 1 (FAF1)expression with Helicobacter pylori (Hp) infection and carcinogenesis of gastric cancer.Methods 8 × 105 cells of human gastric carcinoma cells (HGC-27) and human gastric epithelial cells (GES-1) were co-cultured with Hp standard strain NCTC11637 for 24 h,and the cells were divided by cell/bacterial ratio into three groups:control group,1∶100 and 1∶200 co-culture groups.Each experiment was conducted three times and each sample was assayed in triplicate.Thiazolyl blue (MTT) assay was used to determine the proliferation in 490 nm,flow cytometry to determine the apoptosis of 1 × 104 cells per group,and real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (FQ-PCR) to detect the relative expression of FAF1 mRNA.The sequence length was 164 bp.Results After co-culture with Hp,proliferation of cells was decreased and apoptosis was increased.As compare with respective control group,the relative expression of FAF1 mRNA in 1 ∶ 100 co-culture group and 1 ∶ 200 co-culture group in HGC-27 cells was significantly decreased (0.67 ± 0.20 vs.0.99 ± 0.27,F =11.51,P ＜ 0.05 ; 0.44 ± 0.30 vs.0.99 ±0.27,F =11.5 1,P ＜ 0.01).The relative expression of FAF1 mRNA in 1∶100 co-culture group in GES-1 cells was significantly increased (1.61 ± 0.77 vs.0.99 ± 0.41,F =14.45,P ＜ 0.05),and that in 1∶200 co-culture group in GES-1 cells was significantly decreased (0.36 ±0.22 vs.0.99 ±0.41,F =14.45,P ＜0.05).As compared with control group,1∶100 and 1∶200 co-culture groups of GES-1 cells,the expression levels of FAF1 mRNA in each corresponding group of HGC-27 cells were significantly decreased (0.33±0.16vs.0.99±0.41,t=4.74,P＜0.01; 0.17±0.12 vs.1.08±±0.53,t=5.28,P＜0.01;0.39±0.24 vs.0.98 ±0.23,t=5.67,P＜0.01).Conclusion Hp infection can down-regulate the expression of FAF1 gene,leading to gastric cell proliferation and apoptosis imbalance,then inducing the carcinogenesis of gastric cancer.
