CAJ | 학술논문

目的观察手术对肠道上皮细胞胰岛素信号通路的影响.方法制作阻塞性黄疸大鼠模型,分别于手术前及手术后1、3、24h采血,计算胰岛素抵抗(IR)指数,免疫组织化学检测小肠上皮细胞胰岛素受体表达,Western blot检测肠上皮细胞胰岛素受体底物(IRS)-1、-2及蛋白激酶B(PKB)的表达.结果手术组的IR指数在术后各时间点分别为3.40±0.12、4.29±0.11、5.34±0.18,其中24h值明显高于对照组和假手术组(P＜0.05).手术组的胰岛素受体强阳性表达数分别为3/10、2/10、2/10,其中术后24h较其他时间点下降明显(P＜0.05).手术组术后24h的胰岛素受体底物IRS-1相对表达量为0.42±0.04,较其他组下降明显(P＜0.05),而各组的IRS-2变化不明显.PKB的相对灰度值(0.52±0.10)在手术组中也明显下降(P＜0.05).结论手术应激后IR的发生与肠上皮细胞的胰岛素受体表达降低及受体底物下降有关.PKB是术后IR信号途径中的重要一环.
목적 관찰수술대장도상피세포이도소신호통로적영향.방법 제작조새성황달대서모형,분별우수술전급수술후1、3、24h채혈,계산이도소저항(IR)지수,면역조직화학검측소장상피세포이도소수체표체,Western blot검측장상피세포이도소수체저물(IRS)-1、-2급단백격매B(PKB)적표체.결과 수술조적IR지수재술후각시간점분별위3.40±0.12、4.29±0.11、5.34±0.18,기중24h치명현고우대조조화가수술조(P＜0.05).수술조적이도소수체강양성표체수분별위3/10、2/10、2/10,기중술후24h교기타시간점하강명현(P＜0.05).수술조술후24h적이도소수체저물IRS-1상대표체량위0.42±0.04,교기타조하강명현(P＜0.05),이각조적IRS-2변화불명현.PKB적상대회도치(0.52±0.10)재수술조중야명현하강(P＜0.05).결론 수술응격후IR적발생여장상피세포적이도소수체표체강저급수체저물하강유관.PKB시술후IR신호도경중적중요일배.
Objective To discuss the surgical effects on insulin signaling pathway in intestinal epithelial cells.Methods The obstructive jaundice models of rats were established,then peripheral blood samples were harvested before and at 120 min,4 h and 24 h after surgery,respectively.The insulin resistance (IR) index was calculated.The expression of insulin receptor in intestinal epithelial cells was detected by immunohistochemistry.The expression of the insulin receptor substrate (IRS-1,-2) and protein kinase B (PKB) was tested by Western blotting.Results The IR index in operation group was 3.40 ±0.12,4.29 ±0.11 and 5.34 ±0.18 at 120 min,4 h and 24 h after surgery,respectively.As compared with the control group,the IR index at 24 h was increased significantly in operation group (P ＜ 0.05).The number of strong insulin receptor expression was 3/10,2/10,2/10 at 120 min,4 h and 24 h after surgery respectively,and the expression of insulin receptor at 24 h was reduced obviously (P ＜ 0.05).The expression of IRS-1 was decreased (0.42 ± 0.04,P ＜ 0.05) after operation,but there was no significant change in the IRS-2 expression.The relative amount of PKB was also reduced in operation group (0.52 ±0.10,P ＜ 0.05).Conclusion The occurrence of IR in intestinal epithelial cells is related to the reduced expression of insulin receptor and IRS after surgical stress.The low expression of PKB is an important factor of IR signaling pathway in intestinal epithelial cells.