中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
3期
567-569
,共3页
韩春雷%葛燕%孟凡刚%张建国%张凯%张鑫%史增敏
韓春雷%葛燕%孟凡剛%張建國%張凱%張鑫%史增敏
한춘뢰%갈연%맹범강%장건국%장개%장흠%사증민
癫痫%模型,动物%大鼠%基因芯片%差异表达
癲癇%模型,動物%大鼠%基因芯片%差異錶達
전간%모형,동물%대서%기인심편%차이표체
Epilepsy%Models,animal%Rat%Genechip%Differential expression
目的 应用基因芯片技术检测颞叶癫痫大鼠与正常大鼠海马的基因表达差异,从基因水平探讨癫痫的发病机制.方法 应用立体定向技术建立大鼠颞叶癫痫模型,行电生理及超微结构检测.提取大鼠海马RNA,进行基因芯片杂交,获取芯片数据.对数据进行比较分析,获取差异表达的基因.按照基因功能分类方法,将差异基因进行功能分类.结果 癫痫大鼠模型的脑电及超微结构出现相应的异常改变.按癫痫组与对照组的基因转录产物表达比值＞1.50或＜0.67并且统计量＞2或＜-2为标准筛选,癫痫模型大鼠与正常大鼠比较,差异表达基因113个,其中上调基因46个,下调基因67个.这些差异基因涉及分子功能、生物学进程以及分子组成等功能.结论 基因芯片技术可筛选出癫痫大鼠模型制备前后的差异基因.对差异表达的基因进行分类,可为研究基因功能和传导通路提供基础,为癫痫的治疗提供指导方向.
目的 應用基因芯片技術檢測顳葉癲癇大鼠與正常大鼠海馬的基因錶達差異,從基因水平探討癲癇的髮病機製.方法 應用立體定嚮技術建立大鼠顳葉癲癇模型,行電生理及超微結構檢測.提取大鼠海馬RNA,進行基因芯片雜交,穫取芯片數據.對數據進行比較分析,穫取差異錶達的基因.按照基因功能分類方法,將差異基因進行功能分類.結果 癲癇大鼠模型的腦電及超微結構齣現相應的異常改變.按癲癇組與對照組的基因轉錄產物錶達比值＞1.50或＜0.67併且統計量＞2或＜-2為標準篩選,癲癇模型大鼠與正常大鼠比較,差異錶達基因113箇,其中上調基因46箇,下調基因67箇.這些差異基因涉及分子功能、生物學進程以及分子組成等功能.結論 基因芯片技術可篩選齣癲癇大鼠模型製備前後的差異基因.對差異錶達的基因進行分類,可為研究基因功能和傳導通路提供基礎,為癲癇的治療提供指導方嚮.
목적 응용기인심편기술검측섭협전간대서여정상대서해마적기인표체차이,종기인수평탐토전간적발병궤제.방법 응용입체정향기술건립대서섭협전간모형,행전생리급초미결구검측.제취대서해마RNA,진행기인심편잡교,획취심편수거.대수거진행비교분석,획취차이표체적기인.안조기인공능분류방법,장차이기인진행공능분류.결과 전간대서모형적뇌전급초미결구출현상응적이상개변.안전간조여대조조적기인전록산물표체비치＞1.50혹＜0.67병차통계량＞2혹＜-2위표준사선,전간모형대서여정상대서비교,차이표체기인113개,기중상조기인46개,하조기인67개.저사차이기인섭급분자공능、생물학진정이급분자조성등공능.결론 기인심편기술가사선출전간대서모형제비전후적차이기인.대차이표체적기인진행분류,가위연구기인공능화전도통로제공기출,위전간적치료제공지도방향.
Objective Differential gene expression was investigated with affymetrix genechip expression analysis between temporal lobe epileptic rat and normal rat.Mechanism of epileptic genesis was investigated from gene level.Methods The temporal lobe epileptic rats were established with stereotactic injection with kainic acid (KA) and tested using electrophysiological and ultrastructural methods.Total RNA samples were isolated from hippocampus.After hybridization,washing and staining,the probe arraies were scanned.The data was analyzed to obtain the differentiated genes including up-regulated and downregulated.According to the method of gene function categorizing,the differentiated genes were categorized into different functions.Results The electroencephalography and ultrastructure of the rat model were abnormal.Compared with normal rat,113 common genes were differentially expressed including 46 genes upregulated and 67 down-regulated in temporal lobe epileptic rat.The genes were categorized into different functions such as molecular function,biological process,cellular component etc.Conclusion The different genes between epileptic rat and normal rat can be obtained from Genechip expression analysis.The genes could be categorized into different functions.The mechanism of action about epileptic genesis based on these experiential data of gene ontology and pathway could be explored from gene level,which provides a potential guiding direction for the treatment of epilepsy.
