中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
3期
577-579
,共3页
吴小进%杨辉%米燕燕%殷咏梅
吳小進%楊輝%米燕燕%慇詠梅
오소진%양휘%미연연%은영매
左旋棉酚%多西紫杉醇%肺癌%协同作用
左鏇棉酚%多西紫杉醇%肺癌%協同作用
좌선면분%다서자삼순%폐암%협동작용
Gossypol%Docetaxel%Lung cancer%Synergic effect
目的 观察左旋棉酚(LG)联合多西紫杉醇(DOC)对肺癌治疗的体内体外影响,探讨两者是否具有协同作用及其机制.方法 LG(3、5μmol/L)联合DOC(0.1、1.0 nmoL/L)作用于A549、A549/DDP和A549/T肺癌细胞,采用噻唑蓝(MTT)法和流式细胞仪法分别检测细胞增殖抑制和凋亡诱导作用;建立C57 BL/6J小鼠肺癌Lewis细胞皮下移植瘤模型,将移植瘤小鼠随机分成7组,每组15只,分别为DOC治疗组(1、2 mg/kg)、LG治疗组(3、5 mg/kg)、联合治疗组(3 mg/kg LG+1 mg/kg DOC、5 mg/kg LG +2 mg/kg DOC)和阴性对照组,进行疗效分析.结果 低剂量联合组对A549、A549/DDP和A549/T的增殖抑制率分别为(67.3±3.1)％、(64.8±2.8)％、(54.1±3.3)％,细胞凋亡率分别为(50.2±1.8)％、(52.3±1.5)％、(42.8±2.4)％;高剂量联合组的增殖抑制率分别为(71.8±1.9)％、(72.4±2.1)％、(60.7±2.6)％,细胞凋亡率分别为(53.2±1.8)％、(55.1±1.9)％、(46.7±2.6)％;高低剂量联合组对肺癌细胞的增殖抑制率和细胞凋亡率均比单独使用DOC显著增加(P＜0.05),且联合指数(CI)均小于0.5,表现出强大的协同作用.低高剂量联合组小鼠肺癌Lewis细胞移植瘤的肿瘤抑制率分别为49.23％和66.87％,明显高于DOC治疗组.结论 LG与DOC的联用对肺癌细胞的增殖抑制及诱导凋亡具有协同作用,对克服肿瘤细胞耐药性有良好的效果,对小鼠肺癌Lewis细胞移植瘤有明显的抑制作用,且比单独用药效果显著.
目的 觀察左鏇棉酚(LG)聯閤多西紫杉醇(DOC)對肺癌治療的體內體外影響,探討兩者是否具有協同作用及其機製.方法 LG(3、5μmol/L)聯閤DOC(0.1、1.0 nmoL/L)作用于A549、A549/DDP和A549/T肺癌細胞,採用噻唑藍(MTT)法和流式細胞儀法分彆檢測細胞增殖抑製和凋亡誘導作用;建立C57 BL/6J小鼠肺癌Lewis細胞皮下移植瘤模型,將移植瘤小鼠隨機分成7組,每組15隻,分彆為DOC治療組(1、2 mg/kg)、LG治療組(3、5 mg/kg)、聯閤治療組(3 mg/kg LG+1 mg/kg DOC、5 mg/kg LG +2 mg/kg DOC)和陰性對照組,進行療效分析.結果 低劑量聯閤組對A549、A549/DDP和A549/T的增殖抑製率分彆為(67.3±3.1)％、(64.8±2.8)％、(54.1±3.3)％,細胞凋亡率分彆為(50.2±1.8)％、(52.3±1.5)％、(42.8±2.4)％;高劑量聯閤組的增殖抑製率分彆為(71.8±1.9)％、(72.4±2.1)％、(60.7±2.6)％,細胞凋亡率分彆為(53.2±1.8)％、(55.1±1.9)％、(46.7±2.6)％;高低劑量聯閤組對肺癌細胞的增殖抑製率和細胞凋亡率均比單獨使用DOC顯著增加(P＜0.05),且聯閤指數(CI)均小于0.5,錶現齣彊大的協同作用.低高劑量聯閤組小鼠肺癌Lewis細胞移植瘤的腫瘤抑製率分彆為49.23％和66.87％,明顯高于DOC治療組.結論 LG與DOC的聯用對肺癌細胞的增殖抑製及誘導凋亡具有協同作用,對剋服腫瘤細胞耐藥性有良好的效果,對小鼠肺癌Lewis細胞移植瘤有明顯的抑製作用,且比單獨用藥效果顯著.
목적 관찰좌선면분(LG)연합다서자삼순(DOC)대폐암치료적체내체외영향,탐토량자시부구유협동작용급기궤제.방법 LG(3、5μmol/L)연합DOC(0.1、1.0 nmoL/L)작용우A549、A549/DDP화A549/T폐암세포,채용새서람(MTT)법화류식세포의법분별검측세포증식억제화조망유도작용;건립C57 BL/6J소서폐암Lewis세포피하이식류모형,장이식류소서수궤분성7조,매조15지,분별위DOC치료조(1、2 mg/kg)、LG치료조(3、5 mg/kg)、연합치료조(3 mg/kg LG+1 mg/kg DOC、5 mg/kg LG +2 mg/kg DOC)화음성대조조,진행료효분석.결과 저제량연합조대A549、A549/DDP화A549/T적증식억제솔분별위(67.3±3.1)％、(64.8±2.8)％、(54.1±3.3)％,세포조망솔분별위(50.2±1.8)％、(52.3±1.5)％、(42.8±2.4)％;고제량연합조적증식억제솔분별위(71.8±1.9)％、(72.4±2.1)％、(60.7±2.6)％,세포조망솔분별위(53.2±1.8)％、(55.1±1.9)％、(46.7±2.6)％;고저제량연합조대폐암세포적증식억제솔화세포조망솔균비단독사용DOC현저증가(P＜0.05),차연합지수(CI)균소우0.5,표현출강대적협동작용.저고제량연합조소서폐암Lewis세포이식류적종류억제솔분별위49.23％화66.87％,명현고우DOC치료조.결론 LG여DOC적련용대폐암세포적증식억제급유도조망구유협동작용,대극복종류세포내약성유량호적효과,대소서폐암Lewis세포이식류유명현적억제작용,차비단독용약효과현저.
Objective To study the effects of (-)-gossypol (LG) combined with docetaxel (DOC) for lung cancer by in vivo and in vitro experiments.Methods The inhibition rate of LG (3 and 5 μmol/L) and DOC (0.1 and 1.0 nmol/L) on proliferation of lung cancer cells (A549,A549/DDP and A549/T) was evaluated using methyl thiazolyl tetrazolium (MTT) method,and the cell apoptosis was measured by flow cytometry.C57 BL/6J mice xenograft models were established with Lewis cells.Fifteen mice were randomly divided into 7 groups,including DOC (1 and 2 mg/kg),LG (3 and 5 mg/kg),combined therapy groups (3 mg/kg LG + 1 mg/kg DOC,5 mg/kg LG + 2 mg/kg DOC) and control group,The therapeutic effects were observed.Results In the low dose combination group,the proliferation inhibition rate of lung cancer cells (A549,A549/DDP and A549/T) was (67.3 ± 3.1) ％,(64.8 ± 2.8) ％ and (54.1 ± 3.3) ％,and apoptosis rate was (50.2 ± 1.8) ％,(52.3 ± 1.5) ％ and (42.8 ± 2.4) ％,respectively.In the high dose combination group,the proliferation inhibition rate was (71.8 ± 1.9)％,(72.4±2.1)％ and (60.7 ±2.6)％,and apoptosis rate was (53.2 ± 1.8)％,(55.1 ± 1.9)％ and (46.7 ± 2.6)％,respectively.The proliferation inhibition rate and the apoptosis ratio in combination group were significantly increased (P ＜ 0.05),and CI was less than 0.5 (interpretation strong synergism).In the low and high dose combination group,the tumor inhibition rate was 49.23％ and 66.87％ respectively,which was higher than in the DOC therapy group significantly.Conclusion Combination of LG and DOC has synergistic effect on inhibition of tumor proliferation and apoptosis induction of lung cancer cells,and has favorable effect on surmounting drug resistance of lung cancer,could inhibit mice xenograft tumor of Lewis lung cancer significantly and exert more significant effect than used alone.
