CAJ | 학술논문

目的观察铜绿假单胞菌-甘露糖敏感血凝菌毛株(PA-MSHA)对膀胱癌细胞株的作用,探讨其作用机制.方法使用细胞计数试剂盒(CCK-8)法检测肿瘤细胞的增殖,荧光染色法检测细胞凋亡形态,分光光度法检测凋亡因子半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-2和Caspase-9的水平.结果磷酸盐缓冲液(PBS)组作用T24和5637细胞A450值分别为1.31和1.62,PA-MSHA组A450值分别为0.28和0.41,两组差异有统计学意义(P＜0.05);PA-MSHA处理后的膀胱癌细胞出现凋亡小体;PA-MSHA能够活化T24和5637细胞中Caspase-3及Caspase-9蛋白,与PBS组比较差异有统计学意义(P＜0.05).结论 PA-MSHA可以抑制T24和5637细胞增殖,并诱导凋亡.
목적 관찰동록가단포균-감로당민감혈응균모주(PA-MSHA)대방광암세포주적작용,탐토기작용궤제.방법 사용세포계수시제합(CCK-8)법검측종류세포적증식,형광염색법검측세포조망형태,분광광도법검측조망인자반광안선천동안산특이성단백매(Caspase)-2화Caspase-9적수평.결과 린산염완충액(PBS)조작용T24화5637세포A450치분별위1.31화1.62,PA-MSHA조A450치분별위0.28화0.41,량조차이유통계학의의(P＜0.05);PA-MSHA처리후적방광암세포출현조망소체;PA-MSHA능구활화T24화5637세포중Caspase-3급Caspase-9단백,여PBS조비교차이유통계학의의(P＜0.05).결론 PA-MSHA가이억제T24화5637세포증식,병유도조망.
Objective To investigate the effects of pseudomonas aeruginosa-manno sesensitive hemagglutinin (PA-MSHA) on bladder cancer cells and the mechanism.Methods Cell counting kit-8 (CCK-8) assay was used to measure proliferation of tumor cells.The morphologic changes of apoptotic cells were observed by using immunofluorescence staining.Spectrophotometry was used for detection of Caspase-2 and Caspase-9.Results A450 values on T24 and 5637 cells treated with phosphate buffered saline were 1.31 and 1.62,respectively,and those were 0.28 and 0.41 in PA-MSHA group.As compared with cells treated with phosphate buffer (PBS),5637 or T24 cells treated with PA-MSHA for 24 h exhibited much less cytoplasmic organelles.PA-MSHA could induce activation of Caspase-3 and Caspase-9 in T24 cells and 5637 cells.Conclusion PA-MSHA can inhibit proliferation of T24 and 5637 cells,and induce apoptosis.