中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
3期
634-635
,共2页
金俊硕%高英兰%董玉玺%任志远%李星%陆文浩%李德旭
金俊碩%高英蘭%董玉璽%任誌遠%李星%陸文浩%李德旭
금준석%고영란%동옥새%임지원%리성%륙문호%리덕욱
胰腺导管癌%丛生蛋白%生存素%增殖细胞核抗原%Ki-67
胰腺導管癌%叢生蛋白%生存素%增殖細胞覈抗原%Ki-67
이선도관암%총생단백%생존소%증식세포핵항원%Ki-67
Pancreatic adenocarcinoma%Clusterin%Survivin%Proliferating cell nuclear antigen%Ki-67
目的 探讨在胰腺导管癌中丛生蛋白和抑凋亡蛋白及细胞增殖标志物间的关系.方法 用双重荧光染色法对17例胰腺导管腺癌中丛生蛋白和生存素(Survivin)进行染色并计数阳性细胞进行统计;同法对丛生蛋白和增殖细胞核抗原(PCNA)及Ki-67的表达进行检测.结果 在胰腺导管癌中丛生蛋白阳性细胞数为420个,Survivin阳性细胞数为72个,其中丛生蛋白和Survivin均阳性的细胞数为59个.丛生蛋白、PCNA及Ki-67共同表达的癌细胞数为0个.结论 丛生蛋白在胰腺导管癌中的抗凋亡范围比Survivin更广泛,并且其表达和细胞增殖无相关.
目的 探討在胰腺導管癌中叢生蛋白和抑凋亡蛋白及細胞增殖標誌物間的關繫.方法 用雙重熒光染色法對17例胰腺導管腺癌中叢生蛋白和生存素(Survivin)進行染色併計數暘性細胞進行統計;同法對叢生蛋白和增殖細胞覈抗原(PCNA)及Ki-67的錶達進行檢測.結果 在胰腺導管癌中叢生蛋白暘性細胞數為420箇,Survivin暘性細胞數為72箇,其中叢生蛋白和Survivin均暘性的細胞數為59箇.叢生蛋白、PCNA及Ki-67共同錶達的癌細胞數為0箇.結論 叢生蛋白在胰腺導管癌中的抗凋亡範圍比Survivin更廣汎,併且其錶達和細胞增殖無相關.
목적 탐토재이선도관암중총생단백화억조망단백급세포증식표지물간적관계.방법 용쌍중형광염색법대17례이선도관선암중총생단백화생존소(Survivin)진행염색병계수양성세포진행통계;동법대총생단백화증식세포핵항원(PCNA)급Ki-67적표체진행검측.결과 재이선도관암중총생단백양성세포수위420개,Survivin양성세포수위72개,기중총생단백화Survivin균양성적세포수위59개.총생단백、PCNA급Ki-67공동표체적암세포수위0개.결론 총생단백재이선도관암중적항조망범위비Survivin경엄범,병차기표체화세포증식무상관.
Objective To investigate the correlation between clusterin and inhibitor of apoptosis protein,proliferation markers in pancreatic adenocarcinoma.Methods Double fluorescent staining was employed to detect the expression of clusterin,Survivin,proliferating cell nuclear antigen (PCNA) and Ki-67 in 17 cases of pancreatic adenocarcinoma.Results The numbers of positive cells of clusterin and Survivin were 420 and 72 respectively.Among them,58 were both positive reaction of clusterin and Survivin.However,co-expression was undetected between clusterin and PCNA,Ki-67 in the any cancer cells.Conclusion Clusterin has a wider range than Survivin for anti-apoptosis in pancreatic adenocarcinoma.However,there was no significant association between expression of clusterin and cell proliferation.
