中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
941-943,封3
,共4页
孙言波%刘世海%刘相萍%梁晔%周泉%王海波
孫言波%劉世海%劉相萍%樑曄%週泉%王海波
손언파%류세해%류상평%량엽%주천%왕해파
即刻早期反应基因%肿瘤坏死因子相关凋亡诱导配体%乳腺癌
即刻早期反應基因%腫瘤壞死因子相關凋亡誘導配體%乳腺癌
즉각조기반응기인%종류배사인자상관조망유도배체%유선암
Immediate early response gene X-1%Tumor necrosis factor-related apoptosis inducing ligand%Breast carcinoma
目的 观察即刻早期反应基因(IER3)在肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导乳腺癌细胞HCC1937凋亡中的作用.方法 采用实时定量聚合酶链反应(Real-timePCR)检测TRAIL和IER3干预48 h前后TRAIL和IER3基因表达改变,分别于24、48、72、96、120 h后采用噻唑蓝(MTT)法检测HCC 1937细胞的增殖,10 ～ 14 d后采用细胞克隆形成实验检测细胞克隆形成能力,24 h后采用Hoechst 33258染色检测细胞凋亡.结果 与阴性对照组比较,TRAIL组和IER3+TRAIL组的TRAIL相对表达量分别是42.69±3.88和46.36±3.55;IER3组和IER3+ TRAIL组的IER相对表达量分别是5.08±1.40和9.66±2.25.TRAIL、IER3及TRAIL+ IER3组在120 h时的抑制率(％)分别为(50.95 ±7.45)、(25.39±4.49)及(72.01 ±2.95).IER3+ TRAIL组的细胞克隆形成能力明显降低,而细胞凋亡率明显增高.结论 IER3能增强TRAIL诱导乳腺癌细胞HCC1937的凋亡,两者联合具有协同诱导细胞凋亡的作用.
目的 觀察即刻早期反應基因(IER3)在腫瘤壞死因子相關凋亡誘導配體(TRAIL)誘導乳腺癌細胞HCC1937凋亡中的作用.方法 採用實時定量聚閤酶鏈反應(Real-timePCR)檢測TRAIL和IER3榦預48 h前後TRAIL和IER3基因錶達改變,分彆于24、48、72、96、120 h後採用噻唑藍(MTT)法檢測HCC 1937細胞的增殖,10 ～ 14 d後採用細胞剋隆形成實驗檢測細胞剋隆形成能力,24 h後採用Hoechst 33258染色檢測細胞凋亡.結果 與陰性對照組比較,TRAIL組和IER3+TRAIL組的TRAIL相對錶達量分彆是42.69±3.88和46.36±3.55;IER3組和IER3+ TRAIL組的IER相對錶達量分彆是5.08±1.40和9.66±2.25.TRAIL、IER3及TRAIL+ IER3組在120 h時的抑製率(％)分彆為(50.95 ±7.45)、(25.39±4.49)及(72.01 ±2.95).IER3+ TRAIL組的細胞剋隆形成能力明顯降低,而細胞凋亡率明顯增高.結論 IER3能增彊TRAIL誘導乳腺癌細胞HCC1937的凋亡,兩者聯閤具有協同誘導細胞凋亡的作用.
목적 관찰즉각조기반응기인(IER3)재종류배사인자상관조망유도배체(TRAIL)유도유선암세포HCC1937조망중적작용.방법 채용실시정량취합매련반응(Real-timePCR)검측TRAIL화IER3간예48 h전후TRAIL화IER3기인표체개변,분별우24、48、72、96、120 h후채용새서람(MTT)법검측HCC 1937세포적증식,10 ～ 14 d후채용세포극륭형성실험검측세포극륭형성능력,24 h후채용Hoechst 33258염색검측세포조망.결과 여음성대조조비교,TRAIL조화IER3+TRAIL조적TRAIL상대표체량분별시42.69±3.88화46.36±3.55;IER3조화IER3+ TRAIL조적IER상대표체량분별시5.08±1.40화9.66±2.25.TRAIL、IER3급TRAIL+ IER3조재120 h시적억제솔(％)분별위(50.95 ±7.45)、(25.39±4.49)급(72.01 ±2.95).IER3+ TRAIL조적세포극륭형성능력명현강저,이세포조망솔명현증고.결론 IER3능증강TRAIL유도유선암세포HCC1937적조망,량자연합구유협동유도세포조망적작용.
Objective To observe the effect of immediate early response gene X-1 (IER3) on the apoptosis of the breast cancer cell line HCC1937 induced by tumor necrosis factor-related apoptosis inducing ligand (TRAIL).Methods The expression of gene IRAIL and IER3 was detected before and after treatment by real-time quantitative polymerase chain reaction (Real-time PCR) at 48 h,The proliferation of breast cancer cells HCC1937 was mearsured by using Methylthiazol tetrazolium (MTT) assay at 24,48,72,96,and 120 h,The clone formation ability was mearsuerd by clone formation assay after 10-14 d,breast cancer cells HCC1937 were examined by Hoechst 33258 stain to detect the apoptpsis after 24 h.Results Compared with the negative control group,the relative expression of TRAIL in the TRAIL and the IER3 + TRAIL groups were 42.69 ± 3.88 and 46.36 ± 3.55 times respectively.And the relative expression of the IER3 in the IER3 and the IER3 + TRAIL groups were 5.08 ± 1.40 and 9.66 ± 2.25 times respectively.The inhibition rate (％) of cells in TRAIL,IER3 and TRAIL + IER3 groups were (50.95 ± 7.45),(25.39 ± 4.49) and (72.01 ± 2.95) respectively.The cell clone formation ability in IER3 +TRAIL group was obviously reduced.Compared with the negative control group,the apoptosis rate of cells in IER3 + TRAIL group was also increased significantly.Conclusion IER3 could promote the apoptosis of the breast cancer cell line HCC1937 induced by TRAIL.
