CAJ | 학술논문

目的观察探讨鱼藤素对结肠癌Lovo细胞上皮-间充质转化(EMT)以及侵袭能力的影响.方法分别用Western blot法和实时定量聚合酶链反应(Real-time PCR)法检测鱼藤素对E-钙黏蛋白(E-cadherin)、Twist、Snail、波形蛋白(Vimentin)在蛋白和mRNA水平表达的影响;划痕实验和Transwell侵袭实验检测鱼藤素对Lovo细胞侵袭能力的影响.结果 1μmol/L鱼藤素作用于结肠癌Lovo细胞0、24、48 h后,蛋白水平Twist的相对表达量分别为1.40±0.07、0.41 ±0.07和0.30±0.03;Snail的相对相对表达量分别为1.04±0.11、0.16±0.04和0.23 ±0.02;Vimentin的相对表达量分别为4.31 ±0.30、1.30±0.27和1.21 ±0.21;E-cadherin的相对表达量分别为2.62 ±0.33、3.96±0.27和6.89±0.16.mRNA水平Twist的相对表达量分别为0.63±0.09和0.50±0.06;Snail的相对表达量分别为0.46 ±0.06和0.51 ±0.19;Vimentin的相对表达量分别为0.76±0.05和0.57±0.04;E-cadherin的相对表达量分别为1.88±0.27和2.29 ±0.60.1μmol/L鱼藤素作用于结肠癌Lovo细胞24、48 h后,相对迁移率分别为(20.23±5.16)％和(27.94±6.66)％,而无鱼藤素的对照组24、48 h后相对迁移率则分别为(45.65±4.41)％和(66.14±3.31)％(P＜0.01).Transwell侵袭实验证实鱼藤素组每个视野穿透细胞数为(130.3 ±16.19)个,而对照组为(459.0±14.19)个(P＜0.01).结论鱼藤素能够有效抑制结肠癌Lovo细胞的EMT及侵袭.
목적 관찰탐토어등소대결장암Lovo세포상피-간충질전화(EMT)이급침습능력적영향.방법 분별용Western blot법화실시정량취합매련반응(Real-time PCR)법검측어등소대E-개점단백(E-cadherin)、Twist、Snail、파형단백(Vimentin)재단백화mRNA수평표체적영향;화흔실험화Transwell침습실험검측어등소대Lovo세포침습능력적영향.결과 1μmol/L어등소작용우결장암Lovo세포0、24、48 h후,단백수평Twist적상대표체량분별위1.40±0.07、0.41 ±0.07화0.30±0.03;Snail적상대상대표체량분별위1.04±0.11、0.16±0.04화0.23 ±0.02;Vimentin적상대표체량분별위4.31 ±0.30、1.30±0.27화1.21 ±0.21;E-cadherin적상대표체량분별위2.62 ±0.33、3.96±0.27화6.89±0.16.mRNA수평Twist적상대표체량분별위0.63±0.09화0.50±0.06;Snail적상대표체량분별위0.46 ±0.06화0.51 ±0.19;Vimentin적상대표체량분별위0.76±0.05화0.57±0.04;E-cadherin적상대표체량분별위1.88±0.27화2.29 ±0.60.1μmol/L어등소작용우결장암Lovo세포24、48 h후,상대천이솔분별위(20.23±5.16)％화(27.94±6.66)％,이무어등소적대조조24、48 h후상대천이솔칙분별위(45.65±4.41)％화(66.14±3.31)％(P＜0.01).Transwell침습실험증실어등소조매개시야천투세포수위(130.3 ±16.19)개,이대조조위(459.0±14.19)개(P＜0.01).결론 어등소능구유효억제결장암Lovo세포적EMT급침습.
Objective To investigate the effects of deguelin on epithelial-mesenchymal transition and invasion of rectal cancer lovo cells.Methods Western blotting and real-time quantitative polymerase chain reaction (Real-time PCR) were used to examine the change of E-cadherin,Twist,Snail and Vimen-tin at protein and mRNA levels.Invasive ability was assessed through wound scrach assay and Transwell assay.Results After treated with duguelin 1 μmol/L for 0,24 and 48 h in lovo cells,the relative density ratio of Twist were (1.40 ± 0.07),(0.41 ± 0.07) and (0.30 ± 0.03) respectively ; of Snail were (1.04 ±0.11),(0.16 ± 0.04) and (0.23 ± 0.02) respectively ; of Vimentin were (4.31 ± 0.30),(1.3 ± 0.27)and (1.21 ± 0.21) respectively ; of E-cadherin were (2.62 ± 0.33),(3.96 ± 0.27) and (6.89 ± 0.16)respectively in protein level.In mRNA level,after treated with dugnelin 1 μmol/L for 24 and 48 h in lovo cells,the relative expression of Twist were (0.63 ± 0.09) and (0.50 ± 0.06) respectively ; of Snail were (0.46 ± 0.06) and (0.51 ± 0.19) respectively; of Vimentin were (0.76 ± 0.05) and (0.57 ± 0.04) re-spectively ; of E-cadherin were (1.88 ± 0.27) and (2.29 ± 0.60) respectively.Scrach assay showed that the relative migration rate of Lovo cells treated with duguelin 1μmol/L for 24、48hours were(20.23 ±5.16)％ and(27.94 ±6.66)％ respectively,compared with(45.65 ±4.41)％ and(66.14 ±3.31)％ in control group(P＜0.01).Comparing to (459.0 ± 14 19) cells invaded in control gToup,deguelin decreased invaded cells to (130.3 ± 16.19,P ＜ 0.01).Conclusion The epithelial-mesenchymal transition and invasion of lovo cells can be inhibited strongly by duguelin.