中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
1027-1029
,共3页
李肖珂%裘年存%张勤%单成祥%仇明
李肖珂%裘年存%張勤%單成祥%仇明
리초가%구년존%장근%단성상%구명
十二指肠旷置%能量代谢%胰高血糖素样肽-1受体
十二指腸曠置%能量代謝%胰高血糖素樣肽-1受體
십이지장광치%능량대사%이고혈당소양태-1수체
Duodenal exclusion%Energy metabolism%Glucagon-like peptide-1 receptor
目的 通过植入不同长度的腔内袖管装置(ELS),观察十二指肠肠段旷置对肥胖大鼠能量代谢、血糖调控及胰腺胰高血糖素样肽-1受体(GLP-1R)表达的影响.方法 将雄性SD大鼠随机分为4组:假手术组(SO,n=8)、1 cm ELS管植入组(ELS-1,n=10)、4 cm ELS管植入组(ELS-4,n=8)、10 cm ELS管植入组(ELS-1O,n=9).比较置入ELS组与假手术组的糖尿病大鼠的体质量、能量消耗、胰岛素抵抗、GLP-1水平等多项参数.术后第8周,逆转录-聚合酶链反应(RT-PCR)检测胰腺GLP-1R mRNA的表达变化.比较不同程度的十二指肠旷置对于代谢结果及血糖调控的影响.结果 置入10 cm ELS的大鼠与SO组比较,体质量下降14％(P＜0.05),总能量消耗高13％ (P ＜0.05),静息能量消耗高9％(P＜0.05),胰岛素抵抗明显改善(P＜0.05).空腹GLP-1释放升高(P＜0.05),胰腺GLP-1受体表达水平升高(P＜0.05).而置入4 cm或1 cm ELS的大鼠与SO组比较,在能量消耗上差异无统计学意义(P＞0.05),对血糖稳态的影响轻微(P＜0.05).结论 十二指肠全段旷置能增加大鼠总能量消耗,尤其是静息能量消耗,改善血糖调控能力,提高胰腺GLP-1受体表达,部分旷置只能引起适当的体质量下降.
目的 通過植入不同長度的腔內袖管裝置(ELS),觀察十二指腸腸段曠置對肥胖大鼠能量代謝、血糖調控及胰腺胰高血糖素樣肽-1受體(GLP-1R)錶達的影響.方法 將雄性SD大鼠隨機分為4組:假手術組(SO,n=8)、1 cm ELS管植入組(ELS-1,n=10)、4 cm ELS管植入組(ELS-4,n=8)、10 cm ELS管植入組(ELS-1O,n=9).比較置入ELS組與假手術組的糖尿病大鼠的體質量、能量消耗、胰島素牴抗、GLP-1水平等多項參數.術後第8週,逆轉錄-聚閤酶鏈反應(RT-PCR)檢測胰腺GLP-1R mRNA的錶達變化.比較不同程度的十二指腸曠置對于代謝結果及血糖調控的影響.結果 置入10 cm ELS的大鼠與SO組比較,體質量下降14％(P＜0.05),總能量消耗高13％ (P ＜0.05),靜息能量消耗高9％(P＜0.05),胰島素牴抗明顯改善(P＜0.05).空腹GLP-1釋放升高(P＜0.05),胰腺GLP-1受體錶達水平升高(P＜0.05).而置入4 cm或1 cm ELS的大鼠與SO組比較,在能量消耗上差異無統計學意義(P＞0.05),對血糖穩態的影響輕微(P＜0.05).結論 十二指腸全段曠置能增加大鼠總能量消耗,尤其是靜息能量消耗,改善血糖調控能力,提高胰腺GLP-1受體錶達,部分曠置隻能引起適噹的體質量下降.
목적 통과식입불동장도적강내수관장치(ELS),관찰십이지장장단광치대비반대서능량대사、혈당조공급이선이고혈당소양태-1수체(GLP-1R)표체적영향.방법 장웅성SD대서수궤분위4조:가수술조(SO,n=8)、1 cm ELS관식입조(ELS-1,n=10)、4 cm ELS관식입조(ELS-4,n=8)、10 cm ELS관식입조(ELS-1O,n=9).비교치입ELS조여가수술조적당뇨병대서적체질량、능량소모、이도소저항、GLP-1수평등다항삼수.술후제8주,역전록-취합매련반응(RT-PCR)검측이선GLP-1R mRNA적표체변화.비교불동정도적십이지장광치대우대사결과급혈당조공적영향.결과 치입10 cm ELS적대서여SO조비교,체질량하강14％(P＜0.05),총능량소모고13％ (P ＜0.05),정식능량소모고9％(P＜0.05),이도소저항명현개선(P＜0.05).공복GLP-1석방승고(P＜0.05),이선GLP-1수체표체수평승고(P＜0.05).이치입4 cm혹1 cm ELS적대서여SO조비교,재능량소모상차이무통계학의의(P＞0.05),대혈당은태적영향경미(P＜0.05).결론 십이지장전단광치능증가대서총능량소모,우기시정식능량소모,개선혈당조공능력,제고이선GLP-1수체표체,부분광치지능인기괄당적체질량하강.
Objective By implantating endoluminal sleeve devices at different length,We sought to investigate the influence on energy metabolism,glucose regulation and the expression of glucagon-like peptide-1 receptor (GLP-1R) in the pancreas of diet-induced obese rats.Methods Sprague-Dawley rats were randomly divided into four groups:sham-operated animnals (SO,n =8),rats implanted with a 1 cm endoluminal sleeve devices (ELS) device (ELS-1,n =10),4 cm ELS (ELS-4,n=8),or 10 cm ELS (ELS-10; n =9).We examined the effects of ELS implanted in rats on body weight,energy expenditure,insulin resistance,levels of GLP-1 and so on,comparing them with sham-operated (SO) rats.After 8 weeks,reverse transcriptase-polymerase chain reaction (RT-PCR) was used to analysis the mRNA levels of GLP-1 receptor in the pancreas.To determine the extent of duodenal exclusion required to influence metabolic outcomes and glucose control,we compared the effects of implanting 10,4,or 1 cm ELS with SO.Results ELS-10 rats exhibited 13％ higher total and 9％ higher resting energy expenditure than SO con-trols (P ＜ 0.05).ELS-10 rats also exhibited enhanced fasting GLP-1 secretion and improved GLP-1 receptor expression (P ＜ 0.05),insulin resistance (P ＜ 0.05) with a significantly 14％ lower weight loss (P ＜0.05).While implantation of 4 or 1 cm ELS devices had no effect on energy expenditure and limited effects on glucose homeostasis (P ＞ 0.05).Conclusion Here we report that fully excluding the duode-num with an ELS increased total and resting energy expenditure,improved glucose regulation,as well as the GLP-1 receptor in pancreatic.Exclusion of only a portion of the duodenum resulted in more modest weight loss.
