CAJ | 학술논문

目的观察低功率超声在增强顺铂对食管鳞癌细胞抗肿瘤作用中的影响.方法以食管鳞癌EC9706细胞为实验对象,分别利用单纯低功率超声(0.5 W/cm2和1.0 W/cm2)、单纯顺铂(1 μmol/L)、先超声处理再加入顺铂(超声+顺铂)和先加入顺铂再应用超声(顺铂+超声).4种方式干预细胞的生长,细胞计数试剂盒(CCK-8)法检测细胞活力,显微镜下定性观察荧光染色,流式细胞术定量检测细胞凋亡.结果超声功率分别为0.5W/cm2和1.0W/cm2时,CCK-8法检测细胞活力,与对照组(3.78％、3.97％)比较,低功率超声(14.36％、15.43％)明显降低了肿瘤细胞活力(P＜0.05).超声功率为0.5 W/cm2时,顺铂+超声组(cisPt+ us)细胞凋亡率为(67.23±4.38)％,明显高于超声+顺铂组(us+ cisPt)的(75.38±3.98)％ (P＜0.05).结论低功率超声可以提高肿瘤细胞对化疗药物的敏感性,抗肿瘤药物和超声序贯干预的先后顺序,有助于提高肿瘤杀伤疗效.
목적 관찰저공솔초성재증강순박대식관린암세포항종류작용중적영향.방법 이식관린암EC9706세포위실험대상,분별이용단순저공솔초성(0.5 W/cm2화1.0 W/cm2)、단순순박(1 μmol/L)、선초성처리재가입순박(초성+순박)화선가입순박재응용초성(순박+초성).4충방식간예세포적생장,세포계수시제합(CCK-8)법검측세포활력,현미경하정성관찰형광염색,류식세포술정량검측세포조망.결과 초성공솔분별위0.5W/cm2화1.0W/cm2시,CCK-8법검측세포활력,여대조조(3.78％、3.97％)비교,저공솔초성(14.36％、15.43％)명현강저료종류세포활력(P＜0.05).초성공솔위0.5 W/cm2시,순박+초성조(cisPt+ us)세포조망솔위(67.23±4.38)％,명현고우초성+순박조(us+ cisPt)적(75.38±3.98)％ (P＜0.05).결론 저공솔초성가이제고종류세포대화료약물적민감성,항종류약물화초성서관간예적선후순서,유조우제고종류살상료효.
Objective To explore the antitumor effect of cisplatin combined with low-frequency ultrasound on human esophageal squamous cell carcinoma in vitro.Methods Four modes of treatment were used:exposure to low-power ultrasound,application of cisplatin,exposure to ultrasound followed by cispla-tin,and presence of cisplatin followed by exposure to application ultrasound.Ultrasound was used at inten-sities of 0.5 W/cm2 and 1.0 W/cm2 for 10 min,and cisplatin was applied at concentration of 1 μ mol/L per cell suspension treated in EC9706 cells,respectively.The results of each experimental treatment were assessed by cell counting kit-8 (CCK-8) assay.Flow cytometry was performed to examine the apoptosis rate of cells treated with Annexin V-fluoresceine isothiocyanate (FITC)/proliferation index (PI) double staining.Results The combined effect of ultrasound and cisplatin was more effective than that of ultrasound or cisplatin alone.It also appeared that the order of application played a role,with the cisplatin-ul-trasound treatment lowering cell viability more than the ultrasound-cisplatin treatment.Conclusion Low-frequency ultrasound can promote the cytotoxicity of cisplatin and the order of application played a role in enhancing the sensitivity of chemotherapy drugs on tumor cells.