中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
1060-1062
,共3页
吕瑞涛%法宪恩%朱方涛%黄真锋%余海彬%高勇%赵建明
呂瑞濤%法憲恩%硃方濤%黃真鋒%餘海彬%高勇%趙建明
려서도%법헌은%주방도%황진봉%여해빈%고용%조건명
二氧化铈纳米颗粒%心肌缺血%再灌注损伤%心肌保护
二氧化鈰納米顆粒%心肌缺血%再灌註損傷%心肌保護
이양화시납미과립%심기결혈%재관주손상%심기보호
CeO2 nanoparticles%Myocardial ischemia%Reperfusion injury%Cardioprotection
目的 观察二氧化铈(CeO2)纳米颗粒对大鼠心肌缺血再灌注损伤的保护作用.方法 将SD大鼠50只随机分为假手术组、模型组、1~ 10 nm粒径组、10 ~ 25 nm粒径组、50 nm粒径组,每组10只,制作缺血再灌注模型.采用黄嘌呤氧化酶法和比色法分别测定超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,硫代巴比妥酸法测定丙二醛(MDA)含量,同时观察心肌组织病理学和细胞凋亡.结果 与假手术组比较,模型组SOD、GSH-Px活性明显下降,MDA含量明显升高(P<0.05);与模型组比较,3种不同粒径组SOD、GSH-Px活性明显升高,MDA含量明显降低(P<0.05).3种不同粒径组心肌细胞凋亡指数[1 ~ 10 nm组:(21.00 ±3.90)%;10~25 nm组:(16.38±3.59)%;50 nm组:(24.20±5.88)%]与模型组[(32.92±7.46)%]比较明显降低(P<0.05).结论 二氧化铈纳米颗粒能减轻大鼠缺血再灌注心肌的氧化应激,具有较好的心肌保护作用.
目的 觀察二氧化鈰(CeO2)納米顆粒對大鼠心肌缺血再灌註損傷的保護作用.方法 將SD大鼠50隻隨機分為假手術組、模型組、1~ 10 nm粒徑組、10 ~ 25 nm粒徑組、50 nm粒徑組,每組10隻,製作缺血再灌註模型.採用黃嘌呤氧化酶法和比色法分彆測定超氧化物歧化酶(SOD)和穀胱甘肽過氧化物酶(GSH-Px)活性,硫代巴比妥痠法測定丙二醛(MDA)含量,同時觀察心肌組織病理學和細胞凋亡.結果 與假手術組比較,模型組SOD、GSH-Px活性明顯下降,MDA含量明顯升高(P<0.05);與模型組比較,3種不同粒徑組SOD、GSH-Px活性明顯升高,MDA含量明顯降低(P<0.05).3種不同粒徑組心肌細胞凋亡指數[1 ~ 10 nm組:(21.00 ±3.90)%;10~25 nm組:(16.38±3.59)%;50 nm組:(24.20±5.88)%]與模型組[(32.92±7.46)%]比較明顯降低(P<0.05).結論 二氧化鈰納米顆粒能減輕大鼠缺血再灌註心肌的氧化應激,具有較好的心肌保護作用.
목적 관찰이양화시(CeO2)납미과립대대서심기결혈재관주손상적보호작용.방법 장SD대서50지수궤분위가수술조、모형조、1~ 10 nm립경조、10 ~ 25 nm립경조、50 nm립경조,매조10지,제작결혈재관주모형.채용황표령양화매법화비색법분별측정초양화물기화매(SOD)화곡광감태과양화물매(GSH-Px)활성,류대파비타산법측정병이철(MDA)함량,동시관찰심기조직병이학화세포조망.결과 여가수술조비교,모형조SOD、GSH-Px활성명현하강,MDA함량명현승고(P<0.05);여모형조비교,3충불동립경조SOD、GSH-Px활성명현승고,MDA함량명현강저(P<0.05).3충불동립경조심기세포조망지수[1 ~ 10 nm조:(21.00 ±3.90)%;10~25 nm조:(16.38±3.59)%;50 nm조:(24.20±5.88)%]여모형조[(32.92±7.46)%]비교명현강저(P<0.05).결론 이양화시납미과립능감경대서결혈재관주심기적양화응격,구유교호적심기보호작용.
Objective To investigate the protective effects of cerium oxide (CeO2) nanoparticles on myocardial ischemia-reperfusion injury in rats.Methods Fifty male Sprague-Dawley rats were randomly divided into five groups:sham group,experimental group and three preconditioning groups with different particle diameter [1-10 nm group,10-25 nm group and 50 nm group (n =10 in each group)].The myocardial ischemia-reperfusion model was established successfully.The activities of superoxide dismutase (SOD) and glutathione (GSH)-Px,and the content of malondialdehyde (MDA) in myocardial tissue were determined.The morphology of myocardium was observed under a light microscope,and the apoptosis of myocardial cells was detected by using TdT-mediated dUTP nick end labeling (TUNEL) method.Results As compared with the Sham group,the activities of SOD and GSH-Px in experimental group were significantly decreased (P < 0.05),while the content of MDA was increased (P < 0.05).The activities of SOD and GSH-Px were increased and the content of MDA was decreased in the three different particle diameter groups as compared with the experimental group (P < 0.05).The apoptosis of myocardial cells was significantly decreased in the three different particle diameter groups [(21.00 ± 3.90)% for 1-10 nm group; (16.38 ± 3.59)% for 10-25 nm group; and (24.20 ± 5.88)% for 50 nm group] as compared the experimental group [(32.92 ± 7.46) %] (P < 0.05).Conclusion The CeO2 nanoparticles can protect the myocardium from ischemia-reperfusion injury by attenuating oxidative stress.