中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
1069-1071
,共3页
陆福鼎%许可慰%毕良宽%刘成%玄绪军%黄海
陸福鼎%許可慰%畢良寬%劉成%玄緒軍%黃海
륙복정%허가위%필량관%류성%현서군%황해
膀胱癌%C型凝集素样受体1%卡介苗%核转录因子
膀胱癌%C型凝集素樣受體1%卡介苗%覈轉錄因子
방광암%C형응집소양수체1%잡개묘%핵전록인자
Bladder cancer%Dectin-1%Bacillus calmette-guerin%Nuclear factor-κB
目的 探讨C型凝集素样受体1(Dectin-1)在膀胱癌中的表达及其在卡介苗(BCG)灌注治疗膀胱癌中的作用.方法 分别采用免疫组织化学和Western blot方法检测膀胱癌组织及T24等膀胱癌细胞中Dectin-1的表达;以0、0.1、1.0、2.0、10.0、20.0g/L6个剂量组的BCG分别刺激T24细胞相应时间(15、30 min、1、2、4、8h),在封闭Dectin-1前后采用Western blot法检测其核因子(NF)-κB的激活,并用酶联免疫吸附试验(ELISA)法检测其细胞因子白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α的分泌水平.结果 膀胱癌组织和细胞中均存在Dectin-1的表达;用1.0 g/L的BCG刺激1h后,T24中NF-κB激活最明显,且该浓度组的IL-6及TNF-α分泌水平最高,分别为(29.10 ±2.89)、(21.57±2.88)ng/L;封闭Dectin-1后,BCG刺激效果明显减弱.结论 BCG可能通过膀胱癌上皮表达的Dectin-1受体激活NF-κB,启动免疫反应,发挥防治膀胱癌的作用.
目的 探討C型凝集素樣受體1(Dectin-1)在膀胱癌中的錶達及其在卡介苗(BCG)灌註治療膀胱癌中的作用.方法 分彆採用免疫組織化學和Western blot方法檢測膀胱癌組織及T24等膀胱癌細胞中Dectin-1的錶達;以0、0.1、1.0、2.0、10.0、20.0g/L6箇劑量組的BCG分彆刺激T24細胞相應時間(15、30 min、1、2、4、8h),在封閉Dectin-1前後採用Western blot法檢測其覈因子(NF)-κB的激活,併用酶聯免疫吸附試驗(ELISA)法檢測其細胞因子白細胞介素(IL)-6、腫瘤壞死因子(TNF)-α的分泌水平.結果 膀胱癌組織和細胞中均存在Dectin-1的錶達;用1.0 g/L的BCG刺激1h後,T24中NF-κB激活最明顯,且該濃度組的IL-6及TNF-α分泌水平最高,分彆為(29.10 ±2.89)、(21.57±2.88)ng/L;封閉Dectin-1後,BCG刺激效果明顯減弱.結論 BCG可能通過膀胱癌上皮錶達的Dectin-1受體激活NF-κB,啟動免疫反應,髮揮防治膀胱癌的作用.
목적 탐토C형응집소양수체1(Dectin-1)재방광암중적표체급기재잡개묘(BCG)관주치료방광암중적작용.방법 분별채용면역조직화학화Western blot방법검측방광암조직급T24등방광암세포중Dectin-1적표체;이0、0.1、1.0、2.0、10.0、20.0g/L6개제량조적BCG분별자격T24세포상응시간(15、30 min、1、2、4、8h),재봉폐Dectin-1전후채용Western blot법검측기핵인자(NF)-κB적격활,병용매련면역흡부시험(ELISA)법검측기세포인자백세포개소(IL)-6、종류배사인자(TNF)-α적분비수평.결과 방광암조직화세포중균존재Dectin-1적표체;용1.0 g/L적BCG자격1h후,T24중NF-κB격활최명현,차해농도조적IL-6급TNF-α분비수평최고,분별위(29.10 ±2.89)、(21.57±2.88)ng/L;봉폐Dectin-1후,BCG자격효과명현감약.결론 BCG가능통과방광암상피표체적Dectin-1수체격활NF-κB,계동면역반응,발휘방치방광암적작용.
Objective To study the expression of Dectin-1 in bladder cancer and its roles in bacillus calmette-guerin (BCG) intravesical therapy.Methods Immunohistochemistry and Western blotting were used to detect the expression of Dectin-1 in bladder cancer tissue as well as T24,J82 and EJ cell lines.These cells were stimulated with different concentrations (0,0.1,1.0,2.0,10.0,20.0 g/L) of BCG for 15,30 min,1,2,4 and 8 h,and the nuclear factor-κB (NF-κB) pathway activation was detected before and after blocking Dectin-1 receptor.The levels of cytokines were determined by enzyme linked immunosorbent assay (ELISA).Results Dectin-1 was expressed in the surface of bladder cancer cells.Stimulation with 1.0 g/L of BCG for 1 h could significantly activate NF-κB signaling in T24,and the high-est secretion levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were found in this concentra-tion group,which were separately up to (29.10 ± 2.89) and (21.57 ± 2.88) ng/L,respectively.The BCG stimulating effect could be weakened when Dectin-1 receptor was blocked.Conclusion BCG could activate NF-κB signaling pathway and inflammatory immune response through Dectin-1 receptor,which may play an important role in BCG intravesical therapy.