中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
1079-1080
,共2页
Omega-3多不饱和脂肪酸%雄激素受体%前列腺癌
Omega-3多不飽和脂肪痠%雄激素受體%前列腺癌
Omega-3다불포화지방산%웅격소수체%전렬선암
Omega-3 polyunsaturated fatty acids%Androgen receptor%Prostate cancer
目的 探讨Omega-3多不饱和脂肪酸对前列腺癌细胞生长的作用和机制.方法 通过噻唑蓝(MTT)比色法观察浓度分别为20、40、60、80 μmol/L的二十二碳六烯酸对前列腺癌LNCap细胞活力的影响;使用40 μmol/L二十二碳六烯酸处理前列腺癌LNCap细胞0、12、24、36 h,通过实时荧光定量聚合酶链反应(FQ-PCR)和Western blot法检测二十二碳六烯酸对LNCap细胞雄激素受体(AR)的影响.结果 MTT实验结果显示20、40、60、80 μmol/L的二十二碳六烯酸对LNCap细胞增殖的抑制率分别为13.3％、46.7％、53.3％、63.3％,Western blot结果显示40 μmoL/L的二十二碳六烯酸能够使AR的蛋白表达量下降60％ ～ 80％.结论 Omega-3多不饱和脂肪酸抑制前列腺癌细胞的生长与其下调AR的表达有关.
目的 探討Omega-3多不飽和脂肪痠對前列腺癌細胞生長的作用和機製.方法 通過噻唑藍(MTT)比色法觀察濃度分彆為20、40、60、80 μmol/L的二十二碳六烯痠對前列腺癌LNCap細胞活力的影響;使用40 μmol/L二十二碳六烯痠處理前列腺癌LNCap細胞0、12、24、36 h,通過實時熒光定量聚閤酶鏈反應(FQ-PCR)和Western blot法檢測二十二碳六烯痠對LNCap細胞雄激素受體(AR)的影響.結果 MTT實驗結果顯示20、40、60、80 μmol/L的二十二碳六烯痠對LNCap細胞增殖的抑製率分彆為13.3％、46.7％、53.3％、63.3％,Western blot結果顯示40 μmoL/L的二十二碳六烯痠能夠使AR的蛋白錶達量下降60％ ～ 80％.結論 Omega-3多不飽和脂肪痠抑製前列腺癌細胞的生長與其下調AR的錶達有關.
목적 탐토Omega-3다불포화지방산대전렬선암세포생장적작용화궤제.방법 통과새서람(MTT)비색법관찰농도분별위20、40、60、80 μmol/L적이십이탄륙희산대전렬선암LNCap세포활력적영향;사용40 μmol/L이십이탄륙희산처리전렬선암LNCap세포0、12、24、36 h,통과실시형광정량취합매련반응(FQ-PCR)화Western blot법검측이십이탄륙희산대LNCap세포웅격소수체(AR)적영향.결과 MTT실험결과현시20、40、60、80 μmol/L적이십이탄륙희산대LNCap세포증식적억제솔분별위13.3％、46.7％、53.3％、63.3％,Western blot결과현시40 μmoL/L적이십이탄륙희산능구사AR적단백표체량하강60％ ～ 80％.결론 Omega-3다불포화지방산억제전렬선암세포적생장여기하조AR적표체유관.
Objective To discuss the effect of omega-3 polyunsaturated fatty acids on the growth of prostate cancer cells and the possible action mechanism.Methods The cytotoxic effect of different con-centrations of docosahexenoic acid (20,40,60 and 80 μmol/L) on prostate cancer cell line LNCap was examined by methyl thiazol tetrazolium (MTT) assay.The mRNA and protein expression level of androgen receptor was detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) and Western Blotting in order to demonstrate the effect of 40 μmol/L docosahexenoic acid on the growth of LNCap cells for 0,12,24 and 36 h.Results MTT assay showed that the inhibition ratio of 20,40,60 and 80 μmol/L docosahexenoic acid on the growth of LNCap cells was 13.3％,46.7％,53.3％ and 63.3％ respectively.Western blotting revealed that the androgen receptor protein quantity was obviously downregulated with docosahexenoic acid by 60％-80％.Conclusion Omega-3 Polyunsaturated fatty acids inhibit the growth of prostate cancer cells,which may be associated with down-regulation of androgen recep-tor protein level.
