中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
1128-1130
,共3页
孙淼淼%孙玉生%赵志华%张明智%张威%于庆凯%陈奎生
孫淼淼%孫玉生%趙誌華%張明智%張威%于慶凱%陳奎生
손묘묘%손옥생%조지화%장명지%장위%우경개%진규생
食管鳞癌%检验点激酶1%浸润%转移
食管鱗癌%檢驗點激酶1%浸潤%轉移
식관린암%검험점격매1%침윤%전이
Esophageal squamous carcinoma%Checkpoint kinases 1%Infiltration%Metastasis
目的 探讨细胞周期检测点激酶1(Chk1)在食管鳞癌组织中的表达及其意义.方法 采用免疫组织化学、原位杂交技术分别检测62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织中Chk1蛋白及mRNA的表达,探讨Chk1表达与食管鳞状细胞癌发生发展及浸润转移之间的关系.结果 正常食管黏膜组织、癌旁不典型增生组织及食管鳞癌组织中,Chk1蛋白的阳性表达率分别为14.5％、38.7％和88.7％,三者间两两比较差异均有统计学意义(P＜0.05);其mRNA表达水平亦依次升高,分别为82.3％、35.5％和8.1％,3组间比较差异有统计学意义(P＜0.05);深层浸润组食管鳞癌组织中Chk1的表达(蛋白:94.5％,mRNA:85.5％)均显著高于浅层浸润组(蛋白:42.9％,mRNA:57.1％),两组比较差异有统计学意义(P＜0.05);有淋巴结转移组的食管鳞癌组织中Chk1的表达(蛋白:100.0％,mRNA:95.0％)显著高于无淋巴结转移组(蛋白:83.3％,mRNA:76.2％),两组比较差异有统计学意义(P＜0.05);随着食管鳞癌分化级别的升高,鳞癌组织中Chk1蛋白、mRNA的表达依次升高,组间两两比较差异有统计学意义(P＜0.05).结论 Chk1蛋白及mRNA表达与食管鳞癌发生发展及浸润转移有关.
目的 探討細胞週期檢測點激酶1(Chk1)在食管鱗癌組織中的錶達及其意義.方法 採用免疫組織化學、原位雜交技術分彆檢測62例食管鱗癌組織、31例癌徬不典型增生組織及62例正常食管黏膜組織中Chk1蛋白及mRNA的錶達,探討Chk1錶達與食管鱗狀細胞癌髮生髮展及浸潤轉移之間的關繫.結果 正常食管黏膜組織、癌徬不典型增生組織及食管鱗癌組織中,Chk1蛋白的暘性錶達率分彆為14.5％、38.7％和88.7％,三者間兩兩比較差異均有統計學意義(P＜0.05);其mRNA錶達水平亦依次升高,分彆為82.3％、35.5％和8.1％,3組間比較差異有統計學意義(P＜0.05);深層浸潤組食管鱗癌組織中Chk1的錶達(蛋白:94.5％,mRNA:85.5％)均顯著高于淺層浸潤組(蛋白:42.9％,mRNA:57.1％),兩組比較差異有統計學意義(P＜0.05);有淋巴結轉移組的食管鱗癌組織中Chk1的錶達(蛋白:100.0％,mRNA:95.0％)顯著高于無淋巴結轉移組(蛋白:83.3％,mRNA:76.2％),兩組比較差異有統計學意義(P＜0.05);隨著食管鱗癌分化級彆的升高,鱗癌組織中Chk1蛋白、mRNA的錶達依次升高,組間兩兩比較差異有統計學意義(P＜0.05).結論 Chk1蛋白及mRNA錶達與食管鱗癌髮生髮展及浸潤轉移有關.
목적 탐토세포주기검측점격매1(Chk1)재식관린암조직중적표체급기의의.방법 채용면역조직화학、원위잡교기술분별검측62례식관린암조직、31례암방불전형증생조직급62례정상식관점막조직중Chk1단백급mRNA적표체,탐토Chk1표체여식관린상세포암발생발전급침윤전이지간적관계.결과 정상식관점막조직、암방불전형증생조직급식관린암조직중,Chk1단백적양성표체솔분별위14.5％、38.7％화88.7％,삼자간량량비교차이균유통계학의의(P＜0.05);기mRNA표체수평역의차승고,분별위82.3％、35.5％화8.1％,3조간비교차이유통계학의의(P＜0.05);심층침윤조식관린암조직중Chk1적표체(단백:94.5％,mRNA:85.5％)균현저고우천층침윤조(단백:42.9％,mRNA:57.1％),량조비교차이유통계학의의(P＜0.05);유림파결전이조적식관린암조직중Chk1적표체(단백:100.0％,mRNA:95.0％)현저고우무림파결전이조(단백:83.3％,mRNA:76.2％),량조비교차이유통계학의의(P＜0.05);수착식관린암분화급별적승고,린암조직중Chk1단백、mRNA적표체의차승고,조간량량비교차이유통계학의의(P＜0.05).결론 Chk1단백급mRNA표체여식관린암발생발전급침윤전이유관.
Objective To investigate the expression of checkpoint kinase 1 (Chk1) in esophageal squamous cell carcinoma (ESCC) and its significance.Methods hnmunohistochemistry and in situ hybridization methods were used to detect the expression of Chk1 gene in 62 cases of ESCC,31 cases of adjacent dysplasia and 62 cases of normal esophageal mucosa.Results The expression of Chkl mRNA and protein was gradually increased from normal esophagus mucous tissue (protein:14.5％,mRNA:82.3％),adjacent atypical hyperplasia tissue (protein:38.7％,mRNA:35.5％) to ESCC tissue (pro-tein:88.7％,mRNA:8.1％),and there was significant difference among three groups (P ＜ 0.05).The expression levels of Chk1 mRNA and protein in the deep layer invasive groups (protein:94.5％,mRNA:85.5％) were considerably higher than those in infiltrating shallow layer group (protein:42.9％,mRNA:57.1％),and there was significant difference among the groups (P ＜ 0.05).The expression levels of Chk1 mRNA and protein in lymphatic metastasis groups (protein:100.0％,mRNA:95.0％) were significantly higher than those in non-lymphatic metastasis group (protein:83.3％,mRNA:76.2％),and there was significant difference among the groups (P ＜ 0.05).The expression levels of Chk1 mRNA and protein in poor differentiated ESCC tissues were markedly higher than those in well differentiated tissues (P ＜0.05).Conclusion The expression of Chk1 protein and mRNA was closely associated with the invasion and metastasis of ESCC.
