中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
1147-1149
,共3页
马杰%余杨%蹇召%唐富琴%肖颖彬%陈林
馬傑%餘楊%蹇召%唐富琴%肖穎彬%陳林
마걸%여양%건소%당부금%초영빈%진림
小切口%非人工通气%升主动脉缩窄%左室肥厚%模型,动物
小切口%非人工通氣%升主動脈縮窄%左室肥厚%模型,動物
소절구%비인공통기%승주동맥축착%좌실비후%모형,동물
Small incision%Without artificial ventilation%Ascending aorta stenosis%Left ventricular hypertrophy%Model,animal
目的 探讨非人工通气条件下经胸骨上小切口建立大鼠主动脉瓣上缩窄模型的可行性和有效性.方法 将90只大鼠随机分为主动脉瓣上缩窄组(n=50)和假手术组(n=40),在自主呼吸条件下,经胸骨上窝剪开胸骨至第2肋间,沿气管向下分离出升主动脉并结扎;假手术组仅行开胸处理,记录术前及术后1、3、6、9、12周的心脏超声、组织病理学检查结果,以独立样本t检验等进行比较分析.结果 缩窄组大鼠生存率为84.0％,假手术组为92.5％.超声心动图检查显示:术后3周,缩窄组大鼠左心室呈明显向心性肥厚,舒张期左室后壁厚度为[(2.20±0.24) mm]、左心室重量指数为[(3.11-±0.69) mg/g]与假手术组比较差异有统计学意义(P＜0.05);术后9周,缩窄组大鼠左室呈现离心性肥厚,左室收缩末内径为[(3.25±0.61)mm]、舒张末内径为[(5.64±0.97)mm]显著增加(P＜0.05);术后12周,缩窄组大鼠出现失代偿性心力衰竭的症状,左心室射血分数为[(41.00±9.23)％]显著降低(P＜0.05).组织学检查显示:心肌细胞排列紊乱,细胞核增大,胞质增多,间质胶原纤维增生明显.结论 非人工通气下经胸骨上小切口能成功建立大鼠升主动脉缩窄模型,且该模型具有操作简单、创伤小、重复性好的特点.
目的 探討非人工通氣條件下經胸骨上小切口建立大鼠主動脈瓣上縮窄模型的可行性和有效性.方法 將90隻大鼠隨機分為主動脈瓣上縮窄組(n=50)和假手術組(n=40),在自主呼吸條件下,經胸骨上窩剪開胸骨至第2肋間,沿氣管嚮下分離齣升主動脈併結扎;假手術組僅行開胸處理,記錄術前及術後1、3、6、9、12週的心髒超聲、組織病理學檢查結果,以獨立樣本t檢驗等進行比較分析.結果 縮窄組大鼠生存率為84.0％,假手術組為92.5％.超聲心動圖檢查顯示:術後3週,縮窄組大鼠左心室呈明顯嚮心性肥厚,舒張期左室後壁厚度為[(2.20±0.24) mm]、左心室重量指數為[(3.11-±0.69) mg/g]與假手術組比較差異有統計學意義(P＜0.05);術後9週,縮窄組大鼠左室呈現離心性肥厚,左室收縮末內徑為[(3.25±0.61)mm]、舒張末內徑為[(5.64±0.97)mm]顯著增加(P＜0.05);術後12週,縮窄組大鼠齣現失代償性心力衰竭的癥狀,左心室射血分數為[(41.00±9.23)％]顯著降低(P＜0.05).組織學檢查顯示:心肌細胞排列紊亂,細胞覈增大,胞質增多,間質膠原纖維增生明顯.結論 非人工通氣下經胸骨上小切口能成功建立大鼠升主動脈縮窄模型,且該模型具有操作簡單、創傷小、重複性好的特點.
목적 탐토비인공통기조건하경흉골상소절구건립대서주동맥판상축착모형적가행성화유효성.방법 장90지대서수궤분위주동맥판상축착조(n=50)화가수술조(n=40),재자주호흡조건하,경흉골상와전개흉골지제2륵간,연기관향하분리출승주동맥병결찰;가수술조부행개흉처리,기록술전급술후1、3、6、9、12주적심장초성、조직병이학검사결과,이독립양본t검험등진행비교분석.결과 축착조대서생존솔위84.0％,가수술조위92.5％.초성심동도검사현시:술후3주,축착조대서좌심실정명현향심성비후,서장기좌실후벽후도위[(2.20±0.24) mm]、좌심실중량지수위[(3.11-±0.69) mg/g]여가수술조비교차이유통계학의의(P＜0.05);술후9주,축착조대서좌실정현리심성비후,좌실수축말내경위[(3.25±0.61)mm]、서장말내경위[(5.64±0.97)mm]현저증가(P＜0.05);술후12주,축착조대서출현실대상성심력쇠갈적증상,좌심실사혈분수위[(41.00±9.23)％]현저강저(P＜0.05).조직학검사현시:심기세포배렬문란,세포핵증대,포질증다,간질효원섬유증생명현.결론 비인공통기하경흉골상소절구능성공건립대서승주동맥축착모형,차해모형구유조작간단、창상소、중복성호적특점.
Objective To investigate rat supravalvular aortic stenosis model via small incision on the suprasternal fossa without artificial ventilation.Methods Sprague-Dawley rats were randomly assigned to the aortic banding group (n =50) and the sham-operation group (n =40).The former underwent aortic banding through small incision on the suprastemal fossa without artificial ventilation,and the latter received the same procedures but without actual ligation of the aorta.Echocardiography and myocardial pathological examination were implemented to observe how the heart physiological parameters changed before and after operation.The results were analyzed by using independent sample t test.Results The survival rate was 84％ in banding group,while 92.5％ in sham group.Echocardiography examination showed:(1) At 3rd week after surgical operation,the left ventricle in banding group,which was different from sham-operation group,exhibited obvious concentric hypertrophy.Significant difference in the data on left ventricular posterior wall thick-ness [LVPW,(2.20±0.24) mm] and left ventricular mass index [LVMI,(3.11 ±0.69) mg/g] was observed.The above indicators showed a slight upward trend at 6th,9th,and 12th week after surgical operation; (2) At 9th week after surgical operation,the left ventricle in banding group got eccentric hypertrophy and the values of left ventricular endsystolic dimension [LVESd,(3.25 ±0.61) mm] and left ventricular end-diastolic diameter [LVEDd,(5.64 ± 0.97) mm] increased markedly ; (3) At 12th week later,the symptoms of decompensated heart failure appeared in banding group.The left ventricular ejection fractions [(41.00 ±9.23)％] decreased dramatically.Histological examinations revealed that the banding group exhibited disorganized cardiomyocytes,enlarged nuclei and increased cytoplasmic,and significant interstitial collagen fibers.However,the sham group displayed normal structure.Conclusion These facts confirm that we can effectively establish rat supravalvular aortic stenosis model through this modified method with simple,less-invasive and reproducible features.
