中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
6期
1169-1171
,共3页
张平%董自强%侯毅%袁红纲%董传江
張平%董自彊%侯毅%袁紅綱%董傳江
장평%동자강%후의%원홍강%동전강
膀胱肿瘤%蜂毒素%细胞周期
膀胱腫瘤%蜂毒素%細胞週期
방광종류%봉독소%세포주기
Bladder neoplasm%Melittin%Cell cycle
目的 探讨蜂毒素对膀胱癌细胞株T24细胞周期的作用及其相关调控机制.方法 体外培养膀胱癌T24细胞,经0、7.5、10.0、12.5 mg/L的蜂毒素分组处理后用流式细胞术检测细胞周期,Western blot检测细胞周期蛋白A(Cyclin A)、周期蛋白依赖性激酶2(CDK2)、CDK抑制因子p21cp1和细胞周期蛋白依赖性激酶抑制蛋白27(p27kip1)的蛋白表达变化.结果 膀胱癌T24细胞经不同浓度的蜂毒素处理后各组对应的S期细胞比例分别为(25.73±2.11)％、(31.57±1.91)％、(45.37±0.74)％、(53.67±1.12)％,药物处理组与对照组比较差异有统计学意义(P＜0.01);T24细胞中p21cp1和p27kip1的蛋白表达水平数据增高(p21cicip1:0.1561 ±0.0116、0.209 2±0.010 8、0.287 9±0.012 9、0.351 8±0.013 0;p27kip1:0.250 3±0.012 3、0.342 1 ±0.0123、0.400 3±0.0099、0.469 2±0.0130),而Cyclin A和CDK2的蛋白表达则明显降低(Cyclin A:0.950 7±0.017 8、0.870 6±0.011 9、0.8065 ±0.0120、0.726 7±0.018 4;CDK2:0.965 4±0.014 5、0.868 3±0.015 3、0.755 4±0.008 5、0.652 6±0.0183),与对照组比较差异均有统计学意义(P＜0.01).结论 蜂毒素可导致T24细胞发生S期阻滞,其机制可能与其下调Cyclin A和CDK2的表达,但上调p21cip1与p27kip1的表达有关.
目的 探討蜂毒素對膀胱癌細胞株T24細胞週期的作用及其相關調控機製.方法 體外培養膀胱癌T24細胞,經0、7.5、10.0、12.5 mg/L的蜂毒素分組處理後用流式細胞術檢測細胞週期,Western blot檢測細胞週期蛋白A(Cyclin A)、週期蛋白依賴性激酶2(CDK2)、CDK抑製因子p21cp1和細胞週期蛋白依賴性激酶抑製蛋白27(p27kip1)的蛋白錶達變化.結果 膀胱癌T24細胞經不同濃度的蜂毒素處理後各組對應的S期細胞比例分彆為(25.73±2.11)％、(31.57±1.91)％、(45.37±0.74)％、(53.67±1.12)％,藥物處理組與對照組比較差異有統計學意義(P＜0.01);T24細胞中p21cp1和p27kip1的蛋白錶達水平數據增高(p21cicip1:0.1561 ±0.0116、0.209 2±0.010 8、0.287 9±0.012 9、0.351 8±0.013 0;p27kip1:0.250 3±0.012 3、0.342 1 ±0.0123、0.400 3±0.0099、0.469 2±0.0130),而Cyclin A和CDK2的蛋白錶達則明顯降低(Cyclin A:0.950 7±0.017 8、0.870 6±0.011 9、0.8065 ±0.0120、0.726 7±0.018 4;CDK2:0.965 4±0.014 5、0.868 3±0.015 3、0.755 4±0.008 5、0.652 6±0.0183),與對照組比較差異均有統計學意義(P＜0.01).結論 蜂毒素可導緻T24細胞髮生S期阻滯,其機製可能與其下調Cyclin A和CDK2的錶達,但上調p21cip1與p27kip1的錶達有關.
목적 탐토봉독소대방광암세포주T24세포주기적작용급기상관조공궤제.방법 체외배양방광암T24세포,경0、7.5、10.0、12.5 mg/L적봉독소분조처리후용류식세포술검측세포주기,Western blot검측세포주기단백A(Cyclin A)、주기단백의뢰성격매2(CDK2)、CDK억제인자p21cp1화세포주기단백의뢰성격매억제단백27(p27kip1)적단백표체변화.결과 방광암T24세포경불동농도적봉독소처리후각조대응적S기세포비례분별위(25.73±2.11)％、(31.57±1.91)％、(45.37±0.74)％、(53.67±1.12)％,약물처리조여대조조비교차이유통계학의의(P＜0.01);T24세포중p21cp1화p27kip1적단백표체수평수거증고(p21cicip1:0.1561 ±0.0116、0.209 2±0.010 8、0.287 9±0.012 9、0.351 8±0.013 0;p27kip1:0.250 3±0.012 3、0.342 1 ±0.0123、0.400 3±0.0099、0.469 2±0.0130),이Cyclin A화CDK2적단백표체칙명현강저(Cyclin A:0.950 7±0.017 8、0.870 6±0.011 9、0.8065 ±0.0120、0.726 7±0.018 4;CDK2:0.965 4±0.014 5、0.868 3±0.015 3、0.755 4±0.008 5、0.652 6±0.0183),여대조조비교차이균유통계학의의(P＜0.01).결론 봉독소가도치T24세포발생S기조체,기궤제가능여기하조Cyclin A화CDK2적표체,단상조p21cip1여p27kip1적표체유관.
Objective To investigate the effects of melittin on the cell cycle of bladder cancer cell line T24 and the related regulatory mechanisms.Methods In vitro cultured bladder cancer T24 cells were treated with melittin (0,7.5,10.0,and 12.5 mg/L).Flow cytometry was applied to examine the cell cycle of T24 cells.Western blotting method was applied to detect the protein expression of Cyclin A,cyclindependent kinase 2 (CDK2),CDK inhibitor p21 cip1 and cyclin dependent kinase inhibitor protein 27 (p27kip1).Results Corresponding to different concentration groups of melittin,the proportion of T24 cells in S phase was (25.73 ±2.11)％,(31.57 ±1.91)％,(45.37±0.74)％,and (53.67±1.12)％,showing significant difference between melittin-treated groups and control group (P ＜ 0.01).The protein expression of p21cip1 and p27kip1 in melittin-treated T24 cells was increased (p21cip1:0.156 1 ±0.011 6,0.209 2 ±0.010 8,0.287 9 ±0.012 9,0.351 8 ±0.013 0;p27kip1:0.250 3 ±0.012 3,0.342 1 ±0.012 3,0.400 3 ±0.009 9,0.469 2 ±0.013 0),while that of Cyclin A and CDK2 significantly decreased as compared with control group (Cyclin A:0.950 7 ± 0.017 8,0.870 6 ± 0.011 9,0.806 5 ± 0.012 0,0.726 7 ± 0.018 4;CDK2:0.965 4 ±0.014 5,0.868 3 ±0.015 3,0.755 4 ±0.008 5,0.652 6 ±0.018 3,P ＜0.01).Conclusion Melittin can cause S phase arrest of T24 cells,which may be associated with the down-regulation of Cyclin A and CDK2 expression while up-regulation of p21cip1 and p27kip1.
