中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
6期
1175-1177
,共3页
蒋玉清%孙海滨%王秀丽%郭跃先
蔣玉清%孫海濱%王秀麗%郭躍先
장옥청%손해빈%왕수려%곽약선
糖尿病%胰岛素样生长因子-1%膀胱
糖尿病%胰島素樣生長因子-1%膀胱
당뇨병%이도소양생장인자-1%방광
Diabetes mellitus%Insulin-like growth factor-1%Bladder
目的 应用实时定量聚合酶链反应(Real-time PCR)技术检测胰岛素生长因子-1(IGF-1) mRNA在糖尿病大鼠膀胱的表达变化.方法 将30只SD雄性大鼠随机分成3组,正常对照组10只,糖尿病组10只,治疗组10只.糖尿病组、治疗组大鼠按60 mg/kg腹腔注射链脲佐菌素,72 h后测空腹尾静脉血葡萄糖浓度,血糖浓度＞16.7 mmol/L为糖尿病大鼠造模成功.治疗组SD大鼠给予皮下注射精蛋白锌胰岛素,使血糖维持在3.9～10.0 mmol/L.8周后取大鼠膀胱,提取RNA后应用Real-time PCR方法检测IGF-1 mRNA的△Ct值.结果 糖尿病组IGF-1 mRNA△Ct值为6.141±0.916,正常对照组为4.018±0.491,治疗组为4.922±0.788.△Ct值越低,实际拷贝数越高,基因表达量越高.IGF-1 mRNA在糖尿病组表达量明显下调(P＜0.05).经胰岛素治疗后可上升(P＜0.05).结论 IGF-1 mRNA在糖尿病大鼠膀胱中表达明显下调;经胰岛素治疗后,IGF-1mRNA在糖尿病大鼠膀胱中的表达升高.
目的 應用實時定量聚閤酶鏈反應(Real-time PCR)技術檢測胰島素生長因子-1(IGF-1) mRNA在糖尿病大鼠膀胱的錶達變化.方法 將30隻SD雄性大鼠隨機分成3組,正常對照組10隻,糖尿病組10隻,治療組10隻.糖尿病組、治療組大鼠按60 mg/kg腹腔註射鏈脲佐菌素,72 h後測空腹尾靜脈血葡萄糖濃度,血糖濃度＞16.7 mmol/L為糖尿病大鼠造模成功.治療組SD大鼠給予皮下註射精蛋白鋅胰島素,使血糖維持在3.9～10.0 mmol/L.8週後取大鼠膀胱,提取RNA後應用Real-time PCR方法檢測IGF-1 mRNA的△Ct值.結果 糖尿病組IGF-1 mRNA△Ct值為6.141±0.916,正常對照組為4.018±0.491,治療組為4.922±0.788.△Ct值越低,實際拷貝數越高,基因錶達量越高.IGF-1 mRNA在糖尿病組錶達量明顯下調(P＜0.05).經胰島素治療後可上升(P＜0.05).結論 IGF-1 mRNA在糖尿病大鼠膀胱中錶達明顯下調;經胰島素治療後,IGF-1mRNA在糖尿病大鼠膀胱中的錶達升高.
목적 응용실시정량취합매련반응(Real-time PCR)기술검측이도소생장인자-1(IGF-1) mRNA재당뇨병대서방광적표체변화.방법 장30지SD웅성대서수궤분성3조,정상대조조10지,당뇨병조10지,치료조10지.당뇨병조、치료조대서안60 mg/kg복강주사련뇨좌균소,72 h후측공복미정맥혈포도당농도,혈당농도＞16.7 mmol/L위당뇨병대서조모성공.치료조SD대서급여피하주사정단백자이도소,사혈당유지재3.9～10.0 mmol/L.8주후취대서방광,제취RNA후응용Real-time PCR방법검측IGF-1 mRNA적△Ct치.결과 당뇨병조IGF-1 mRNA△Ct치위6.141±0.916,정상대조조위4.018±0.491,치료조위4.922±0.788.△Ct치월저,실제고패수월고,기인표체량월고.IGF-1 mRNA재당뇨병조표체량명현하조(P＜0.05).경이도소치료후가상승(P＜0.05).결론 IGF-1 mRNA재당뇨병대서방광중표체명현하조;경이도소치료후,IGF-1mRNA재당뇨병대서방광중적표체승고.
Objective To observe the insulin-like growth factor-1 (IGF-1) mRNA expression in diabetic rat bladder.Methods Thirty Sprague-Dawley (SD) male rats were randomly divided into 3 groups:normal control group,diabetic group,and treatment group,n =10 each.The rats in diabetic group and treatment group were injected intraperitoneally with 60 mg/kg Stretopzin (STZ).After 72 h,fasting blood glucose was measure,and BG ＞ 16.7 mmol/L indicated the successful establishment of diabetic model.SD rats in treatment group were given subcutaneous injection of protamine zinc insulin,so BG remained at 3.9-10.0 mmol/L.Bladders of three groups were obtained after 8 weeks,The mRNA from bladders was extracted for detection of △Ct of IGF-1 mRNA by real-time quantitative polymerase chain reaction (Real-time PCR).Results IGF-1 mRNA △Ct in the diabetic group,normal control group and treatment group was 6.141 ±0.916,4.018 ±0.491 and 4.922 ±0.788.the lower the Delta Ct value was lower,the greater the actual copy number was and the higher the amount of gene expression.Conclusion IGF-1 mRNA expression in diabetic rat bladder was significantly reduced.The insulin treatment may up-regulate the IGF-1 mRNA expression in the bladders of diabetic rats.
