中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
6期
1202-1203
,共2页
方烈奎%袁谦%蒋兴粒%黄向江%黄建生%熊星%朱莹%杨江根
方烈奎%袁謙%蔣興粒%黃嚮江%黃建生%熊星%硃瑩%楊江根
방렬규%원겸%장흥립%황향강%황건생%웅성%주형%양강근
肾细胞癌%基因诊断%钙黏蛋白-6
腎細胞癌%基因診斷%鈣黏蛋白-6
신세포암%기인진단%개점단백-6
Renal cell carcinomas%Gene diagnosis%Cadherin-6
目的 探讨肾细胞癌患者外周血中钙黏蛋白-6 mRNA的表达及其临床意义.方法 采用逆转录-聚合酶链反应(RT-PCR)法检测30例肾细胞癌患者、15例正常健康人外周血中钙黏蛋白-6mRNA的表达.结果 肾细胞癌患者外周血中钙黏蛋白-6 mRNA表达的阳性率为46.7％,与正常健康人(0)比较差异有统计学意义(P＜0.01),钙黏蛋白-6 mRNA的表达率随着临床病理分期的增高而提高.结论 应用RT-PCR法检测肾癌患者外周血钙黏蛋白-6 mRNA的表达较为敏感.
目的 探討腎細胞癌患者外週血中鈣黏蛋白-6 mRNA的錶達及其臨床意義.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)法檢測30例腎細胞癌患者、15例正常健康人外週血中鈣黏蛋白-6mRNA的錶達.結果 腎細胞癌患者外週血中鈣黏蛋白-6 mRNA錶達的暘性率為46.7％,與正常健康人(0)比較差異有統計學意義(P＜0.01),鈣黏蛋白-6 mRNA的錶達率隨著臨床病理分期的增高而提高.結論 應用RT-PCR法檢測腎癌患者外週血鈣黏蛋白-6 mRNA的錶達較為敏感.
목적 탐토신세포암환자외주혈중개점단백-6 mRNA적표체급기림상의의.방법 채용역전록-취합매련반응(RT-PCR)법검측30례신세포암환자、15례정상건강인외주혈중개점단백-6mRNA적표체.결과 신세포암환자외주혈중개점단백-6 mRNA표체적양성솔위46.7％,여정상건강인(0)비교차이유통계학의의(P＜0.01),개점단백-6 mRNA적표체솔수착림상병리분기적증고이제고.결론 응용RT-PCR법검측신암환자외주혈개점단백-6 mRNA적표체교위민감.
Objective To investigate the expression of Cadherin-6 mRNA and its clinical significance in peripheral blood of patients with renal cell carcinoma (RCC).Methods The expression of Cadherin-6 mRNA in 30 cases of RCC and 15 normal controls was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results The positive rate of Cadherin-6 mRNA was 46.7％ in peripheral blood of RCC patients,significantly higher than in normal controls (0) (P ＜ 0.001).The expression of Cadherin-6 mRNA was increased with the increase of clinicopathologic stage.Conclusion The expression of Cadherin-6 mRNA in peripheral blood of patients with RCC is a sensitive indicator by RT-PCR.
