中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
6期
1231-1234
,共4页
蔡建良%姚炜敏%苑学礼%夏溟%那彦群
蔡建良%姚煒敏%苑學禮%夏溟%那彥群
채건량%요위민%원학례%하명%나언군
良性前列腺增生%胆碱能神经%去神经支配%自噬%大鼠
良性前列腺增生%膽堿能神經%去神經支配%自噬%大鼠
량성전렬선증생%담감능신경%거신경지배%자서%대서
Benign prostate hyperplasia%Cholinergic innervation%Denervation%Autophagy%Rat
目的 观察脱特罗定持续干扰胆碱能神经支配对大鼠良性增生前列腺腺上皮的影响.方法 将29周龄雄性自发性高血压(SHR)大鼠42只,分为实验组18只,对照组18只,正常对照组6只.实验组每天2次脱特罗定(3.5 mg/kg体质量,溶解于1.0 ml生理盐水)灌胃给药,对照组每天2次胃内灌入等量的生理盐水,正常对照组不做任何处理.每12周处死一批大鼠,比较各组SHR大鼠前列腺腹侧叶大体形态、湿重/体质量比值、干重/湿重比值,组织切片光镜、透射电镜下观察,免疫组织化学LC3-Ⅱ染色等.结果 各阶段实验组SHR大鼠前列腺腹侧叶大体形态与对照组差异不明显,两组大鼠前列腺腹侧叶湿重/体质量比值随周龄均进行性升高,各阶段两者差异均无统计学意义(P＞0.05);对照组大鼠前列腺腹侧叶干重/湿重比值随周龄进行性升高,但实验组升高趋势不明显,41周龄后两组差异有统计学意义(P＜0.01);光镜下观察,对照组大鼠前列腺腺上皮细胞继续呈现良性增生状态且进行性明显,而实验组大鼠前列腺腺上皮细胞逐渐出现萎缩性改变,41周龄后两组差异较为明显;电镜下观察,对照组前列腺腺上皮细胞呈现随周龄进行性明显的细胞增生和分泌功能亢进,腺细胞胞质内自噬体、自噬前体和自噬溶酶体结构众多;而实验组腺上皮细胞增生表现和胞质内自噬体结构随大鼠周龄的增加而逐渐下降,41周龄后两组差异有统计学意义(P＜0.01);典型的细胞凋亡现象在实验组和对照组各阶段差异均不明显;免疫组织化学实验发现,实验组大鼠前列腺腺上皮细胞胞质LC3-Ⅱ/LC3-Ⅰ阳性染色较同周龄对照组大鼠显著下降,其差异亦随大鼠周龄的延长而更明显,41周龄后差异有统计学意义(P＜0.01).实验组大鼠与正常对照组比较,上述差异均无统计学意义(P＞0.05).结论 药物持续干扰前列腺胆碱能神经支配后,大鼠良性增生前列腺腺上皮细胞逐渐出现萎缩改变,该病理过程与腺上皮细胞内自噬活性的显著下降可能有重要关系.
目的 觀察脫特囉定持續榦擾膽堿能神經支配對大鼠良性增生前列腺腺上皮的影響.方法 將29週齡雄性自髮性高血壓(SHR)大鼠42隻,分為實驗組18隻,對照組18隻,正常對照組6隻.實驗組每天2次脫特囉定(3.5 mg/kg體質量,溶解于1.0 ml生理鹽水)灌胃給藥,對照組每天2次胃內灌入等量的生理鹽水,正常對照組不做任何處理.每12週處死一批大鼠,比較各組SHR大鼠前列腺腹側葉大體形態、濕重/體質量比值、榦重/濕重比值,組織切片光鏡、透射電鏡下觀察,免疫組織化學LC3-Ⅱ染色等.結果 各階段實驗組SHR大鼠前列腺腹側葉大體形態與對照組差異不明顯,兩組大鼠前列腺腹側葉濕重/體質量比值隨週齡均進行性升高,各階段兩者差異均無統計學意義(P＞0.05);對照組大鼠前列腺腹側葉榦重/濕重比值隨週齡進行性升高,但實驗組升高趨勢不明顯,41週齡後兩組差異有統計學意義(P＜0.01);光鏡下觀察,對照組大鼠前列腺腺上皮細胞繼續呈現良性增生狀態且進行性明顯,而實驗組大鼠前列腺腺上皮細胞逐漸齣現萎縮性改變,41週齡後兩組差異較為明顯;電鏡下觀察,對照組前列腺腺上皮細胞呈現隨週齡進行性明顯的細胞增生和分泌功能亢進,腺細胞胞質內自噬體、自噬前體和自噬溶酶體結構衆多;而實驗組腺上皮細胞增生錶現和胞質內自噬體結構隨大鼠週齡的增加而逐漸下降,41週齡後兩組差異有統計學意義(P＜0.01);典型的細胞凋亡現象在實驗組和對照組各階段差異均不明顯;免疫組織化學實驗髮現,實驗組大鼠前列腺腺上皮細胞胞質LC3-Ⅱ/LC3-Ⅰ暘性染色較同週齡對照組大鼠顯著下降,其差異亦隨大鼠週齡的延長而更明顯,41週齡後差異有統計學意義(P＜0.01).實驗組大鼠與正常對照組比較,上述差異均無統計學意義(P＞0.05).結論 藥物持續榦擾前列腺膽堿能神經支配後,大鼠良性增生前列腺腺上皮細胞逐漸齣現萎縮改變,該病理過程與腺上皮細胞內自噬活性的顯著下降可能有重要關繫.
목적 관찰탈특라정지속간우담감능신경지배대대서량성증생전렬선선상피적영향.방법 장29주령웅성자발성고혈압(SHR)대서42지,분위실험조18지,대조조18지,정상대조조6지.실험조매천2차탈특라정(3.5 mg/kg체질량,용해우1.0 ml생리염수)관위급약,대조조매천2차위내관입등량적생리염수,정상대조조불주임하처리.매12주처사일비대서,비교각조SHR대서전렬선복측협대체형태、습중/체질량비치、간중/습중비치,조직절편광경、투사전경하관찰,면역조직화학LC3-Ⅱ염색등.결과 각계단실험조SHR대서전렬선복측협대체형태여대조조차이불명현,량조대서전렬선복측협습중/체질량비치수주령균진행성승고,각계단량자차이균무통계학의의(P＞0.05);대조조대서전렬선복측협간중/습중비치수주령진행성승고,단실험조승고추세불명현,41주령후량조차이유통계학의의(P＜0.01);광경하관찰,대조조대서전렬선선상피세포계속정현량성증생상태차진행성명현,이실험조대서전렬선선상피세포축점출현위축성개변,41주령후량조차이교위명현;전경하관찰,대조조전렬선선상피세포정현수주령진행성명현적세포증생화분비공능항진,선세포포질내자서체、자서전체화자서용매체결구음다;이실험조선상피세포증생표현화포질내자서체결구수대서주령적증가이축점하강,41주령후량조차이유통계학의의(P＜0.01);전형적세포조망현상재실험조화대조조각계단차이균불명현;면역조직화학실험발현,실험조대서전렬선선상피세포포질LC3-Ⅱ/LC3-Ⅰ양성염색교동주령대조조대서현저하강,기차이역수대서주령적연장이경명현,41주령후차이유통계학의의(P＜0.01).실험조대서여정상대조조비교,상술차이균무통계학의의(P＞0.05).결론 약물지속간우전렬선담감능신경지배후,대서량성증생전렬선선상피세포축점출현위축개변,해병리과정여선상피세포내자서활성적현저하강가능유중요관계.
Objective To investigate the changes of rats' benign hyperplasia prostate glandular epithelium cells after continuing blockade the cholinergic innervation by drug.Methods All 42 male spontaneous hypertension rats (SHR,a spontaneous BPH animal model,29 weeks old) were divided into experiment group (EG),control group (CG) and normal control (NC).EG were intragastric administered with Tolterodine (3.5 mg/kg,bid),CG were intragastric administered with equivalent physiological saline.Rats were sacrificed in batches every 12 weeks,the changes of the prostate gross morphology,ratio of ventral prostate lobe wet weight and body weight (wet/body),ratio of ventral prostate lobe dry weight and wet weight (wet/dry),histology,ultra-structure and LC3-Ⅱ & LC3-Ⅰ expression were observed.Results The gross morphological changes and the increase of wet/body ratio of EG rats' prostate were similar to CG in every stage.The wet/body ratio of two group increased with rats' age,but EG were significantly smaller than CG after 41 weeks.The proliferation of prostate glandular epithelium cell in EG were stopped while in CG were continuing.The number of autophagosome was increased with age in CG glandular cell cytoplasm,while in EG,the number of autophagosome was decreased with age.The immunohistrochemistry staining of LC3-Ⅰ progressively attenuated and LC3-Ⅱ staining progressively reinforced with the rat' s age in CG,but in EG,the situation were conversed.All these index between CG and NC,had no statistical difference.Conclusion The benign hyperplasia of rats' prostate glandular epithelium cells have been reversed after blockade the cholinergic innervation by drug,the significantly inactivation of autophagy may play an important role.
