CAJ | 학술논문

目的观察谷胱甘肽S-转移酶P1(GSTPl)表达对心肌微血管内皮细胞(CMECs)迁移、增殖及凋亡能力的影响.方法以酶消化法获得SD大鼠CMECs,传代培养后,采用质粒转染CMECs获得GSTP1过表达细胞株,采用Transwell法检测细胞的迁移能力;用噻唑蓝(MTT)比色法检测细胞的增殖能力,同时采用流式细胞仪检测细胞的凋亡.结果与对照组比较,GSTP1过表达后CMECs的迁移能力明显减弱[(9.0±2.7)个比(12.8±2.5)个,P＜0.05],增殖能力也明显下降,但细胞凋亡指数明显增加(0.26 ±0.02比0.05±0.01,P＜0.05).结论 GSTP1能有效抑制CMECs的迁移、增殖,并促进其凋亡.
목적 관찰곡광감태S-전이매P1(GSTPl)표체대심기미혈관내피세포(CMECs)천이、증식급조망능력적영향.방법 이매소화법획득SD대서CMECs,전대배양후,채용질립전염CMECs획득GSTP1과표체세포주,채용Transwell법검측세포적천이능력;용새서람(MTT)비색법검측세포적증식능력,동시채용류식세포의검측세포적조망.결과 여대조조비교,GSTP1과표체후CMECs적천이능력명현감약[(9.0±2.7)개비(12.8±2.5)개,P＜0.05],증식능력야명현하강,단세포조망지수명현증가(0.26 ±0.02비0.05±0.01,P＜0.05).결론 GSTP1능유효억제CMECs적천이、증식,병촉진기조망.
Objective To detect the effect of glutahione S-transferases (GSTP1) expression on the migration,proliferation and apoptosis of cardiac microvascular endothelial cells (CMECs).Methods After obtaining CMECs from SD rats by enzymatic digestion method,the special plasmid of GSTP was transfected.The ability of migration,proliferation and apoptosis of CMECs were examined by using Transwell assay,methyl thiazol tetrazolium (MTT) assay and flow cytometry,respectively.Results The ability of migration of CMECs was significantly reduced in GSTP1 over-expression group as compared with control group (9.0 ±2.7 vs.12.8 ±2.5,P ＜0.05),and the ability of proliferation of CMECs was also obviously reduced in comparison with control group (0.26 ± 0.02 vs.0.05 ± 0.01,P ＜ 0.05).But the apoptotic index was increased significantly (P ＜ 0.05).Conclusion GSTP1 expression can effectively inhibit migration and proliferation,and promote apoptosis of CMECs.