CAJ | 학술논문

目的检测微小RNA(miRNA,miR)-500在丙型肝炎肝硬化到肝癌病程中的动态表达,探讨miR-500表达变化在丙型肝炎肝硬化再到丙型肝炎相关性肝癌病变过程中的作用机制.方法选取本院收治8例丙型肝炎肝硬化患者为肝硬化组,8例丙型肝炎病毒感染相关性肝癌患者为肝癌组,另选取8名健康体检者为对照组,利用实时定量聚合酶链反应(Real-time PCR)法测定上述3组人群血浆中miR-500的含量.结果肝硬化组中血浆miR-500表达水平为5.72±2.28,高于对照组的0.95±0.48,两者差异有统计学意义(P＜0.05);肝癌组患者血浆中miR-500表达水平为18.62 ±8.13,高于对照组和肝硬化组,两者差异有统计学意义(P＜0.05).结论 miR-500可能参与丙型肝炎肝硬化到肝癌的发生发展过程.
목적 검측미소RNA(miRNA,miR)-500재병형간염간경화도간암병정중적동태표체,탐토miR-500표체변화재병형간염간경화재도병형간염상관성간암병변과정중적작용궤제.방법 선취본원수치8례병형간염간경화환자위간경화조,8례병형간염병독감염상관성간암환자위간암조,령선취8명건강체검자위대조조,이용실시정량취합매련반응(Real-time PCR)법측정상술3조인군혈장중miR-500적함량.결과 간경화조중혈장miR-500표체수평위5.72±2.28,고우대조조적0.95±0.48,량자차이유통계학의의(P＜0.05);간암조환자혈장중miR-500표체수평위18.62 ±8.13,고우대조조화간경화조,량자차이유통계학의의(P＜0.05).결론 miR-500가능삼여병형간염간경화도간암적발생발전과정.
Objective To investigate the dysregulation of microRNA-500 (miR-500) in the development process from hepatitis C liver cirrhosis to hepatocellular carcinoma so as to clarify the role of change of miR-500 expression in the development process from the hepatitis C to liver cirrhosis and hepatocellular carcinoma.Methods From Nov.2012 to Oct.2013,8 cases of hepatitis C liver cirrhosis and 8 cases of hepatitis C virus infection-related liver cancer were selected as the research objects,and 8 cases of the healthy check-up served as the control group.The expression level of miR-500 in the plasma of the above three groups was tested by using real-time quantitative polymerase chain reaction (Real-time PCR).Results The expression level of miR-500 in liver cirrhosis group was 5.72 ± 2.28,higher than 0.95 ± 0.48 in the control group,and that in liver cancer group was 18.62 ± 8.13,higher than in the control group and liver cirrhosis group.Conclusion The miR-500 may be involved in the development process from chronic hepatitis C liver cirrhosis to liver cancer.