CAJ | 학술논문

目的探讨LIM和SH3蛋白1(Lasp1) mRNA及蛋白水平在人胃癌组织中的表达及其临床意义.方法分别采用实时荧光定量聚合酶链反应(FQ-PCR)、Western blot和免疫组织化学(IHC)方法,检测Lasp1在82例胃癌患者的癌组织及配对癌旁正常组织标本中mRNA和蛋白表达水平,探讨其与胃癌患者不同临床病理指标之间的关系.结果人胃癌组织较癌旁正常胃黏膜组织Lasp1 mRNA(3.098 ±0.215比2.147±0.104,P＜0.05)和蛋白(3.223±0.210比2.134±0.160,P＜0.05)表达水平均显著升高.Lasp1 mRNA水平在淋巴结转移阳性(1.64±1.52比3.92±1.47,P＜0.05)及晚期(Ⅲ+Ⅳ期)胃癌(1.95±1.32比3.48±1.39,P＜0.05)患者中表达明显增高;同样,免疫组织化学结果显示Lasp1蛋白在淋巴结转移阳性(Z=-2.078,P＜0.05)及晚期(Z=-2.382,P＜0.05)胃癌患者中表达明显增高,Lasp1 mRNA及蛋白表达水平与胃癌病理分级、肿瘤大小无明显相关(P＞0.05).结论 Lasp1在人胃癌组织中表达增高,其表达水平的升高可能与胃癌的进展和淋巴结转移呈正相关.
목적 탐토LIM화SH3단백1(Lasp1) mRNA급단백수평재인위암조직중적표체급기림상의의.방법 분별채용실시형광정량취합매련반응(FQ-PCR)、Western blot화면역조직화학(IHC)방법,검측Lasp1재82례위암환자적암조직급배대암방정상조직표본중mRNA화단백표체수평,탐토기여위암환자불동림상병리지표지간적관계.결과 인위암조직교암방정상위점막조직Lasp1 mRNA(3.098 ±0.215비2.147±0.104,P＜0.05)화단백(3.223±0.210비2.134±0.160,P＜0.05)표체수평균현저승고.Lasp1 mRNA수평재림파결전이양성(1.64±1.52비3.92±1.47,P＜0.05)급만기(Ⅲ+Ⅳ기)위암(1.95±1.32비3.48±1.39,P＜0.05)환자중표체명현증고;동양,면역조직화학결과현시Lasp1단백재림파결전이양성(Z=-2.078,P＜0.05)급만기(Z=-2.382,P＜0.05)위암환자중표체명현증고,Lasp1 mRNA급단백표체수평여위암병리분급、종류대소무명현상관(P＞0.05).결론 Lasp1재인위암조직중표체증고,기표체수평적승고가능여위암적진전화림파결전이정정상관.
Objective To investigate the gene transcript level and protein expression of LIM and SH3 Domain protein 1 (Lasp1) in gastric cancer tissues and their clinical significance.Methods The mRNA level and protein expression of Lasp1 in 82 cases of gastric cancer tissues and the paired normal gastric tissues were detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR),Western blotting and immunohistochemistry (IHC) respectively.The relationship between the Lasp1 expression level and the various clinicopathological parameters was analyzed.Results The Lasp1 mRNA (3.098 ± 0.215 vs.2.147 ±0.104,P ＜0.05) and protein expression (3.223 ±0.210 vs.2.134 ±0.160,P ＜0.05) in cancerous tissues was increased significantly as compared to the normal gastric tissues.Though there was no significant correlation between Lasp1 expression with tumor size and pathological differential degrees,Lasp1 mRNA level was significantly higher in patients with lymph node metastasis (N1-3) (3.92 ± 1.47 vs.1.64 ± 1.52,P ＜ 0.05) and those with more advanced clinical stages (3.48 ± 1.39 vs.1.95 ± 1.32,P ＜ 0.05) respectively.Consistently,IHC revealed that patients with lymph node metastasis (Z =-2.078,P ＜ 0.05) and those with more advanced clinical stages (Z =-2.382,P ＜ 0.05) were prone to show higher Lasp1 protein level.Conclusion Above results suggested that Lasp1 was overexpressed in gastric cancer tissues,which may contribute to cancerous progression and lymph node metastasis.