中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
6期
1334-1336,封4
,共4页
胡春华%易贤林%刘泽贞%陈雁如%钟惟德%张炎
鬍春華%易賢林%劉澤貞%陳雁如%鐘惟德%張炎
호춘화%역현림%류택정%진안여%종유덕%장염
痔%微血管密度%血管内皮生长因子%血管内皮生长因子受体2
痔%微血管密度%血管內皮生長因子%血管內皮生長因子受體2
치%미혈관밀도%혈관내피생장인자%혈관내피생장인자수체2
Hemorrhoids%Microvessel density%Vascular endothelial growth factor%Vascular endothelial growth factor receptor 2
目的 观察微血管密度与血管内皮生长因子(VEGF)及其受体2(VEGFR2)在痔黏膜及黏膜下组织中的表达的关系,以探讨其与临床表现的关系.方法 利用免疫组织化学染色技术,对比研究60例痔疮患者和20例非痔患者的正常肛垫和痔两种组织内微血管的变化,并检测VEGF及其受体VEGFR2的表达与微血管密度的关系,同时对比临床Ⅲ、Ⅳ分度的痔疮患者微血管VEGF和VEGFR2的表达区别.结果 痔组织与正常组织对比存在显著的新生血管形成,痔组织中VEGF、VEGFR2染色平均评分分别为(2.83±0.12)、(2.07 ±0.11)分,与相对正常组织比较,差异有统计学意义(P＜0.01);按临床表现分类,Ⅲ、Ⅳ度两组对比,VEGF染色评分差异有统计学意义(P =0.027).结论 VEGF-VEGFR在微血管发展中起着重要的作用,VEGF的表达与痔患者临床表现密切相关.
目的 觀察微血管密度與血管內皮生長因子(VEGF)及其受體2(VEGFR2)在痔黏膜及黏膜下組織中的錶達的關繫,以探討其與臨床錶現的關繫.方法 利用免疫組織化學染色技術,對比研究60例痔瘡患者和20例非痔患者的正常肛墊和痔兩種組織內微血管的變化,併檢測VEGF及其受體VEGFR2的錶達與微血管密度的關繫,同時對比臨床Ⅲ、Ⅳ分度的痔瘡患者微血管VEGF和VEGFR2的錶達區彆.結果 痔組織與正常組織對比存在顯著的新生血管形成,痔組織中VEGF、VEGFR2染色平均評分分彆為(2.83±0.12)、(2.07 ±0.11)分,與相對正常組織比較,差異有統計學意義(P＜0.01);按臨床錶現分類,Ⅲ、Ⅳ度兩組對比,VEGF染色評分差異有統計學意義(P =0.027).結論 VEGF-VEGFR在微血管髮展中起著重要的作用,VEGF的錶達與痔患者臨床錶現密切相關.
목적 관찰미혈관밀도여혈관내피생장인자(VEGF)급기수체2(VEGFR2)재치점막급점막하조직중적표체적관계,이탐토기여림상표현적관계.방법 이용면역조직화학염색기술,대비연구60례치창환자화20례비치환자적정상항점화치량충조직내미혈관적변화,병검측VEGF급기수체VEGFR2적표체여미혈관밀도적관계,동시대비림상Ⅲ、Ⅳ분도적치창환자미혈관VEGF화VEGFR2적표체구별.결과 치조직여정상조직대비존재현저적신생혈관형성,치조직중VEGF、VEGFR2염색평균평분분별위(2.83±0.12)、(2.07 ±0.11)분,여상대정상조직비교,차이유통계학의의(P＜0.01);안림상표현분류,Ⅲ、Ⅳ도량조대비,VEGF염색평분차이유통계학의의(P =0.027).결론 VEGF-VEGFR재미혈관발전중기착중요적작용,VEGF적표체여치환자림상표현밀절상관.
Objective To observe the microvessel density and the expression of vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR2) in mucosa and submucosal tissue of the patients with hemorrhoids and study its relationship with clinical severity.Methods Sixty cases of hemorrhoids and 20 cases of no-hemorrhoids as control group were enrolled.The expression of VEGF and VEGFR2,and the microvessel density were detected by immunohistochemical staining (IHC) and scored according to the IHC result.The clinical relevance of the expression of VEGF,VEGFR2 and the microvessel density with the clinical indexing of hemorrhoid patients was analyzed.Results As contrasted with control group,neovascularization in hemorrhoid group was obviously increased.As compared with the control group,the average staining score of VEGF and VEGFR2 was 2.83 ± 0.12 and 2.07 ± 0.11 in hemorrhoid group (P ＜ 0.01 for both).There was significant difference in the VEGF staining score (P ＜ 0.05) between degree Ⅲ hemorrhoid and degree Ⅳ hemorrhoid.Conclusion VEGF-VEGFR plays an important role in the microvascular development of hemorrhoids,and the expression of VEGF is related with the clinical severity.
