CAJ | 학술논문

目的检测食管鳞癌组织中人表皮生长因子受体2(HER2)/neu基因扩增和蛋白表达,并探讨其与食管鳞癌临床病理特征的关系.方法采用荧光原位杂交(FISH)和免疫组织化学(IHC)方法检测120例食管鳞癌组织中HER2/neu基因扩增及蛋白表达水平.结果 FISH结果示食管鳞癌组织中HER2/neu基因扩增率为4.17％ (5/120),IHC示HER2蛋白过表达(+++)率为3.33％ (4/120),基因扩增与蛋白表达明显相关(P＜0.01);HER2/neu基因扩增和蛋白过表达与食管鳞癌患者的临床病理特征均无相关(P＞0.05).结论食管鳞癌组织HER2/neu基因扩增及蛋白表达率均较低,其在食管鳞癌中的意义有待进一步的研究.
목적 검측식관린암조직중인표피생장인자수체2(HER2)/neu기인확증화단백표체,병탐토기여식관린암림상병리특정적관계.방법 채용형광원위잡교(FISH)화면역조직화학(IHC)방법검측120례식관린암조직중HER2/neu기인확증급단백표체수평.결과 FISH결과시식관린암조직중HER2/neu기인확증솔위4.17％ (5/120),IHC시HER2단백과표체(+++)솔위3.33％ (4/120),기인확증여단백표체명현상관(P＜0.01);HER2/neu기인확증화단백과표체여식관린암환자적림상병리특정균무상관(P＞0.05).결론 식관린암조직HER2/neu기인확증급단백표체솔균교저,기재식관린암중적의의유대진일보적연구.
Objective To investigate human epidermalgrowth factor receptor-2 (HER2)/neu gene amplification and protein expression in esophageal squamous cell carcinoma (ESCC) and to explore their clinicopathological correlations.Methods The HER2/neu gene status and protein expression in 120 cases of ESCC were evaluated by using fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC).Results HER2/neu gene amplification and HER2 protein overexpression (+++) were found in 5 (4.17％) and 4 (3.33％) cases of ESCC respectively.HER2 overexpression was associated significantly with HER2/neu gene amplification.There was no correlation between the HER2/neu gene amplification or overexpression of HER2 protein and the ESCC patients' clinicopathological features.Conclusion The HER2/neu gene amplification rate and its protein overexpression rate in ESCC were low,and the role HER2/neu gene plays in ESCC still need further studies.