CAJ | 학술논문

目的检测食管鳞癌及其癌前病变组织中Yes相关蛋白(YAP)的表达水平,探讨YAP与食管癌发生的关系和临床意义.方法应用免疫组织化学方法分别检测125例食管癌及其配对的正常食管黏膜,23例不典型增生组织中YAP的表达水平.结果 YAP在不典型增生(34.8％)、食管癌组织(50.4％)中的阳性表达率显著高于正常食管黏膜组织(12.8％,P＜0.01);YAP蛋白阳性表达率与肿瘤部位、年龄、性别无相关(p＞0.05),但与肿瘤分化程度和淋巴结转移之间有关(P＜0.05).结论 YAP的表达与食管鳞癌的发生与发展相关.
목적 검측식관린암급기암전병변조직중Yes상관단백(YAP)적표체수평,탐토YAP여식관암발생적관계화림상의의.방법 응용면역조직화학방법분별검측125례식관암급기배대적정상식관점막,23례불전형증생조직중YAP적표체수평.결과 YAP재불전형증생(34.8％)、식관암조직(50.4％)중적양성표체솔현저고우정상식관점막조직(12.8％,P＜0.01);YAP단백양성표체솔여종류부위、년령、성별무상관(p＞0.05),단여종류분화정도화림파결전이지간유관(P＜0.05).결론 YAP적표체여식관린암적발생여발전상관.
Objective To investigate the expression of Yes-associated protein (YAP) in human esophageal squamous carcinoma and precancerous lesions,and its clinical significance.Methods The expression of YAP protein was detected by PV9000 immunohistochemical method in 125 esophageal squamous carcinoma specimens,23 cases of dysplasia and 125 cases of normal esophageal mucosa.Results The positive rate of YAP protein in esophageal squamous carcinoma specimens (50.4％) and dysplasia (34.8％) was significantly higher than that in normal esophageal mucosa (12.8％,P ＜ 0.01).YAP protein expression was not significantly associated with tumor location,age,or gender,but significantly correlated with tumor differentiation and stage (both P ＜ 0.05).Conclusion YAP is related to the occurrence and progression of human esophageal squamous carcinoma and may be used as an accessory parameter for early diagnosis and predicting the prognosis of esophageal squamous carcinoma.