CAJ | 학술논문

目的应用葡聚硫酸钠(DSS)和偶氮氧甲烷(AOM)建立小鼠从炎症性肠病(IBD)至肠道肿瘤的动态模型过程.方法 54只BALB/c小鼠随机分为对照组(A组)、单剂量循环DSS组(B组)和DSS循环联合AOM组(C组).结果在实验第1、4、7周末,B、C组小鼠体质量与A组比较均明显下降(P＜0.05);在实验第8、9周末,C组小鼠体质量增长数与B组比较明显下降(P＜0.05).在第3个循环末(第9周),B组小鼠的病理切片主要表现为慢性炎症,部分小鼠有轻度不典型增生;C组大部分小鼠病理切片有肿瘤形成.结论 AOM一次性腹腔注射联合DSS三循环喂养可建立小鼠从炎症性肠病发展到肿瘤的动态全过程.
목적 응용포취류산납(DSS)화우담양갑완(AOM)건립소서종염증성장병(IBD)지장도종류적동태모형과정.방법 54지BALB/c소서수궤분위대조조(A조)、단제량순배DSS조(B조)화DSS순배연합AOM조(C조).결과 재실험제1、4、7주말,B、C조소서체질량여A조비교균명현하강(P＜0.05);재실험제8、9주말,C조소서체질량증장수여B조비교명현하강(P＜0.05).재제3개순배말(제9주),B조소서적병리절편주요표현위만성염증,부분소서유경도불전형증생;C조대부분소서병리절편유종류형성.결론 AOM일차성복강주사연합DSS삼순배위양가건립소서종염증성장병발전도종류적동태전과정.
Objective To establish a dynamic process to tumors in mice from inflammatory bowel disease (IBD) induce by dextran sodium sulfate (DSS) and azoxymethane (AOM).Methods Three groups were used:normal group (A),single-dose DSS group (B) and DSS with AOM group (C).Results At 1,4,7 week during the experiment,group B and group C mice decreased body weight compared with group A (P ＜ 0.05) ; At 8,9 week,the weight gain of group C was significantly less than that of group B (P ＜ 0.05).At the end of the third cycle (week 9),the majority of group B biopsies showed chronic inflammation,Most of group C mice showed the tumor.Conclusion AOM intraperitoneal injection with 3 cycles of drinking DSS solution can establish from inflammatory bowel disease to tumor development dynamic model.