CAJ | 학술논문

目的探讨门静脉高压症侧枝血管形成过程中是否有内皮祖细胞(EPCs)的参与.方法建立大鼠门静脉高压症食管静脉曲张模型,共15例,为实验组;假手术大鼠5例,为对照组.比较两组间门静脉血中EPCs数量的差异,模型组大鼠门静脉血中EPCs数量与食管组织血管密度的关系.免疫组织化学法检测模型组大鼠及人体食管静脉表达CD133.结果门脉高压症食管静脉曲张模型组EPCs克隆数量[(17.5±4.9)个]明显较正常对照组多[(6.3±2.7)个,P＜0.叭];EPCs细胞克隆数量与食管组织血管密度密切相关(P＜0.01).在门静脉高压症食管静脉曲张模型大鼠食管黏膜下血管中可见CD133阳性的内皮细胞,而对照组无阳性发现.结论 EPCs可能参与了门脉高压症食管静脉新血管的形成.
목적 탐토문정맥고압증측지혈관형성과정중시부유내피조세포(EPCs)적삼여.방법 건립대서문정맥고압증식관정맥곡장모형,공15례,위실험조;가수술대서5례,위대조조.비교량조간문정맥혈중EPCs수량적차이,모형조대서문정맥혈중EPCs수량여식관조직혈관밀도적관계.면역조직화학법검측모형조대서급인체식관정맥표체CD133.결과 문맥고압증식관정맥곡장모형조EPCs극륭수량[(17.5±4.9)개]명현교정상대조조다[(6.3±2.7)개,P＜0.팔];EPCs세포극륭수량여식관조직혈관밀도밀절상관(P＜0.01).재문정맥고압증식관정맥곡장모형대서식관점막하혈관중가견CD133양성적내피세포,이대조조무양성발현.결론 EPCs가능삼여료문맥고압증식관정맥신혈관적형성.
Objective To investigate the involvement of endothelial progenitor cells (EPCs) in collateral angiogenesis in portal hypertension.Methods A rat model of portal hypertension with esophagogastric varices was established (n =15) as model group,and the rats were subjected to sham operations as control group (n =5).The counts of EPCs in the portal veins in the two groups and the relationship between the EPCs and vessel density in esophageal tissue in the model group were analyzed.The expression of CD133 in the esophageal veins of rats with esophagogastric varices was detected by the immunohistochemical method.Results Significantly more EPCs colonies were observed in the model group than in the control group (P ＜ 0.01),and this finding was closely correlated with the vascular density of the esophageal tissue (P ＜ 0.01).CD133-positive endothelial cells were visible in the esophageal submucosal vessels of model group but not in control group.Conclusion EPCs may be involved in the angiogenesis of esophageal veins in rats with portal hypertension.