CAJ | 학술논문

目的观察卵巢切除对硫酸鱼精蛋白介导的间质性膀胱炎(IC)大鼠模型中膀胱肥大细胞的影响,探讨雌激素、孕激素在IC发病中的作用.方法将30只雌性成年SD大鼠随机分为3组:卵巢切除+硫酸鱼精蛋白(OVX+ PS)组、假手术+PS(Sham+ PS)组和正常对照组.OVX+ PS组和Sham+ PS组膀胱内灌注PS建立IC膀胱黏膜损伤机制大鼠模型,正常对照组膀胱内灌注生理盐水.60 d后处死大鼠并切取膀胱,苏木素-伊红(HE)染色观察膀胱黏膜变化及炎性细胞浸润,甲苯胺蓝染色标记并计数肥大细胞,分析肥大细胞活性.结果 PS灌注组可见膀胱黏膜损伤,黏膜下可见炎性细胞浸润.OVX+ PS组膀胱黏膜固有层和逼尿肌肥大细胞计数分别为(2.770±1.112)、(1.180±0.598)个/高倍镜视野,Sham+ PS组肥大细胞计数分别为(2.640±1.022)、(0.990±0.630)个/高倍镜视野,与正常对照组[(1.160±0.523)、(0.330±0.177)个/高倍镜视野]比较均明显增多(P＜0.01);OVX+ PS组与Sham+ PS组比较肥大细胞计数差异无统计学意义(P＞0.05).OVX+ PS组膀胱黏膜固有层和逼尿肌脱颗粒肥大细胞百分比分别为(27.46±15.69)％和(36.93±19.64)％,与Sham+ PS组[(90.94±8.46)％、(80.58±24.18)％]比较明显降低(P＜0.01);与正常对照组[(22.92±9.52)％、(24.95±18.81)％]比较,OVX+ PS组膀胱黏膜固有层和逼尿肌的脱颗粒肥大细胞百分比差异均无统计学意义(P＞0.05),而Sham+ PS组脱颗粒肥大细胞百分比均明显增加(P＜0.01).结论 PS大鼠膀胱灌注可构建较为典型的IC动物模型;卵巢切除可显著抑制IC模型中膀胱肥大细胞的活化及脱颗粒.
목적 관찰란소절제대류산어정단백개도적간질성방광염(IC)대서모형중방광비대세포적영향,탐토자격소、잉격소재IC발병중적작용.방법 장30지자성성년SD대서수궤분위3조:란소절제+류산어정단백(OVX+ PS)조、가수술+PS(Sham+ PS)조화정상대조조.OVX+ PS조화Sham+ PS조방광내관주PS건립IC방광점막손상궤제대서모형,정상대조조방광내관주생리염수.60 d후처사대서병절취방광,소목소-이홍(HE)염색관찰방광점막변화급염성세포침윤,갑분알람염색표기병계수비대세포,분석비대세포활성.결과 PS관주조가견방광점막손상,점막하가견염성세포침윤.OVX+ PS조방광점막고유층화핍뇨기비대세포계수분별위(2.770±1.112)、(1.180±0.598)개/고배경시야,Sham+ PS조비대세포계수분별위(2.640±1.022)、(0.990±0.630)개/고배경시야,여정상대조조[(1.160±0.523)、(0.330±0.177)개/고배경시야]비교균명현증다(P＜0.01);OVX+ PS조여Sham+ PS조비교비대세포계수차이무통계학의의(P＞0.05).OVX+ PS조방광점막고유층화핍뇨기탈과립비대세포백분비분별위(27.46±15.69)％화(36.93±19.64)％,여Sham+ PS조[(90.94±8.46)％、(80.58±24.18)％]비교명현강저(P＜0.01);여정상대조조[(22.92±9.52)％、(24.95±18.81)％]비교,OVX+ PS조방광점막고유층화핍뇨기적탈과립비대세포백분비차이균무통계학의의(P＞0.05),이Sham+ PS조탈과립비대세포백분비균명현증가(P＜0.01).결론 PS대서방광관주가구건교위전형적IC동물모형;란소절제가현저억제IC모형중방광비대세포적활화급탈과립.
Objective To investigate the role of female hormones in the pathogenesis of interstitial cystitis by the study of ovariectomy on the bladder mast cells in animal models of interstitial cystitis.Methods A total of 30 female adult Sprague-Dawley rats were divided into 3 groups,including bilateral ovariectomy plus protamine sulfate(OVX + PS) group,sham operation plus protamine sulfate control (Sham +PS) group and control group.The OVX + PS group and Sham + PS group were instilled with 50 g/L protamine sulfate once every two days,and the control group was instilled with normal saline.After intravesical instillation for 60 days,the rats were killed and the bladders were removed for toluidine blue staining to count the number of mast cell and to investigate the form of mast cells.Results In the groups of PS instilled,the bladder mucosa was damaged,and inflammatory cells could be seen in lamina propria.In OVX + PS group,the number of mast cells in lamina propria and detrusor was (2.770 ± 1.112)/HP and (1.180 ±0.598)/HP respectively,and the number was (2.640 ± 1.022)/HP and (0.990 ±0.630)/HP in Sham + PS group.Both of the number were obviously higher than those in control group [(1.160 ± 0.523)/HP and (0.330 ± 0.177)/HP,respectively],and the difference was statistically significant(P ＜ 0.01).However,compared to OVX + PS group and Sham + PS group,the difference was not statistically significant (P ＞ 0.05).the OVX + PS group showed significant lower of the percentage of degranulated mast cells [(27.46 ± 15.69) ％ and (36.93 ± 19.64)％ in lamina propria and detrusor respectively] than those in Sham + PS group [(90.94 ± 8.46)％ and (80.58 ± 24.18)％],and the difference was statistically significant (P＜0.01).Compared to control group [(22.92 ± 9.52) ％ and (24.95 ± 18.81) ％],the percentage of degranulated mast cells in Sham + PS group was obviously higher,and the difference was statistically significant (P ＜0.01).Compared to OVX + PS group and control group,the difference was not statistically significant (P ＞ 0.05) in both lamina propria and detrusor.Conclusion Bilateral ovariectomy could significantly inhibit the degranulated of mast cells in bladder of IC animal models.