中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
9期
1968-1970
,共3页
郑小青%郑浩%吕夷松%韦胜威%李怀富
鄭小青%鄭浩%呂夷鬆%韋勝威%李懷富
정소청%정호%려이송%위성위%리부부
膀胱%纤维化%转化生长因子-β%炎症
膀胱%纖維化%轉化生長因子-β%炎癥
방광%섬유화%전화생장인자-β%염증
Bladder%Fibrosis%Transforming growth factor-β%Inflammation
目的 观察转化生长因子-β1(TGF-β1)通过Smad-3对膀胱慢性炎症纤维化的调节作用.方法 将40只SD大鼠随机分为对照组、模型组、大剂量及小剂量干预组.通过尿流体动力学、逆转录-聚合酶链反应(RT-PCR)、苏木素-伊红(HE)及Masson三色染色检测各组差异.结果 与对照组比较,模型组大鼠膀胱慢性炎症纤维化显著,膀胱顺应性由(0.99±0.11) ml/mmHg降低为(0.29±0.05) ml/mmHg,膀胱TGF-β1、Smad-3、Ⅰ型胶原(Collagen Ⅰ)、Ⅲ型胶原(CollagenⅢ)的mRNA表达均显著升高(0.79±0.07、0.73 ±0.06、1.09 ±0.09、1.32 ±0.05,P＜0.05).两个剂量SB431542干预后,大鼠膀胱顺应性分别增高到(0.64±0.07)和(0.71±0.05) ml/mmHg,Smad-3表达降低为0.30±0.04和0.25±0.05,膀胱炎症及纤维化改善(P＜0.05).结论 TGF-β1通过调节Smad-3在膀胱慢性炎症及纤维化进程中发挥促进作用.
目的 觀察轉化生長因子-β1(TGF-β1)通過Smad-3對膀胱慢性炎癥纖維化的調節作用.方法 將40隻SD大鼠隨機分為對照組、模型組、大劑量及小劑量榦預組.通過尿流體動力學、逆轉錄-聚閤酶鏈反應(RT-PCR)、囌木素-伊紅(HE)及Masson三色染色檢測各組差異.結果 與對照組比較,模型組大鼠膀胱慢性炎癥纖維化顯著,膀胱順應性由(0.99±0.11) ml/mmHg降低為(0.29±0.05) ml/mmHg,膀胱TGF-β1、Smad-3、Ⅰ型膠原(Collagen Ⅰ)、Ⅲ型膠原(CollagenⅢ)的mRNA錶達均顯著升高(0.79±0.07、0.73 ±0.06、1.09 ±0.09、1.32 ±0.05,P＜0.05).兩箇劑量SB431542榦預後,大鼠膀胱順應性分彆增高到(0.64±0.07)和(0.71±0.05) ml/mmHg,Smad-3錶達降低為0.30±0.04和0.25±0.05,膀胱炎癥及纖維化改善(P＜0.05).結論 TGF-β1通過調節Smad-3在膀胱慢性炎癥及纖維化進程中髮揮促進作用.
목적 관찰전화생장인자-β1(TGF-β1)통과Smad-3대방광만성염증섬유화적조절작용.방법 장40지SD대서수궤분위대조조、모형조、대제량급소제량간예조.통과뇨류체동역학、역전록-취합매련반응(RT-PCR)、소목소-이홍(HE)급Masson삼색염색검측각조차이.결과 여대조조비교,모형조대서방광만성염증섬유화현저,방광순응성유(0.99±0.11) ml/mmHg강저위(0.29±0.05) ml/mmHg,방광TGF-β1、Smad-3、Ⅰ형효원(Collagen Ⅰ)、Ⅲ형효원(CollagenⅢ)적mRNA표체균현저승고(0.79±0.07、0.73 ±0.06、1.09 ±0.09、1.32 ±0.05,P＜0.05).량개제량SB431542간예후,대서방광순응성분별증고도(0.64±0.07)화(0.71±0.05) ml/mmHg,Smad-3표체강저위0.30±0.04화0.25±0.05,방광염증급섬유화개선(P＜0.05).결론 TGF-β1통과조절Smad-3재방광만성염증급섬유화진정중발휘촉진작용.
Objective To evaluate the effects of transforming growth factor-β1 (TGF-β1) and Smad-3 on chronic cystitis and fibrosis.Methods Forty Sprague-Dawley rats were randomized into control group,model group,low dose group and large dose group.Urodynamic detection,real time reverse transcription polymerase chain reaction,hematoxylin and eosin staining and Masson staining were done to determine the difference among the groups.Results As compared with control group,chronic cystitis with diffuse fibrosis in model group was aggravated,bladder compliance was increased [(0.99 ± 0.11) ml/mmHg to (0.29 ± 0.05) m]/mmHg],and the mRNA levels of TGF-β1,Smad-3,Collagen Ⅰ,Collagen Ⅲ increased were significantly (0.79 ±0.07,0.73 ±0.06,1.09 ±0.09,1.32 ±0.05,P ＜0.05).After administration of two doses of SB431542,bladder compliance was increased significantly [(0.64 ± 0.07) and (0.71 ± 0.05) m]/mmHg],the mRNA levels of Smad-3 decreased significantly (0.30 ± 0.04 and 0.25 ± 0.05),while the inflammation and fibrosis reduced (P ＜ 0.05).Conclusion TGF-β1 promotes the bladder inflammatory fibrosis probably through the Smad-3 pathway.
