中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
9期
1986-1988
,共3页
骨肉瘤%肿瘤干细胞%N糖基化%巨噬细胞
骨肉瘤%腫瘤榦細胞%N糖基化%巨噬細胞
골육류%종류간세포%N당기화%거서세포
Osteosarcoma%Cancer stem-like cells%N-glycan%Macrophages
目的 检测骨肉瘤类肿瘤干细胞对巨噬细胞表型转换的影响.方法 苦马豆素预处理的CD133+ CD44+骨肉瘤细胞LM8与骨髓巨噬细胞共培养后检测巨噬细胞表型相关标志分子的表达.结果 与未处理组比较,1 mg/L苦马豆素处理组巨噬细胞精氨酸酶(Arg)-1[(12.0±3.1)％比(40.0±2.6)％,P＜0.05]和白细胞介素(IL)-10[(90.0±4.4) ng/L比(150.0 ±6.8) ng/L,P＜0.05]表达下降,诱导型一氧化氮合酶(iNOS)[(50.0±2.1)％比(12.0±1.3)％,P＜0.05]与肿瘤坏死因子(TNF)-α[(240.0 ±8.1)ng/L比(50.0±3.3) ng/L,P＜0.05]表达上升.结论 骨肉瘤类肿瘤干细胞高表达1,6分支N-糖链诱导巨噬细胞M2表型转换.
目的 檢測骨肉瘤類腫瘤榦細胞對巨噬細胞錶型轉換的影響.方法 苦馬豆素預處理的CD133+ CD44+骨肉瘤細胞LM8與骨髓巨噬細胞共培養後檢測巨噬細胞錶型相關標誌分子的錶達.結果 與未處理組比較,1 mg/L苦馬豆素處理組巨噬細胞精氨痠酶(Arg)-1[(12.0±3.1)％比(40.0±2.6)％,P＜0.05]和白細胞介素(IL)-10[(90.0±4.4) ng/L比(150.0 ±6.8) ng/L,P＜0.05]錶達下降,誘導型一氧化氮閤酶(iNOS)[(50.0±2.1)％比(12.0±1.3)％,P＜0.05]與腫瘤壞死因子(TNF)-α[(240.0 ±8.1)ng/L比(50.0±3.3) ng/L,P＜0.05]錶達上升.結論 骨肉瘤類腫瘤榦細胞高錶達1,6分支N-糖鏈誘導巨噬細胞M2錶型轉換.
목적 검측골육류류종류간세포대거서세포표형전환적영향.방법 고마두소예처리적CD133+ CD44+골육류세포LM8여골수거서세포공배양후검측거서세포표형상관표지분자적표체.결과 여미처리조비교,1 mg/L고마두소처리조거서세포정안산매(Arg)-1[(12.0±3.1)％비(40.0±2.6)％,P＜0.05]화백세포개소(IL)-10[(90.0±4.4) ng/L비(150.0 ±6.8) ng/L,P＜0.05]표체하강,유도형일양화담합매(iNOS)[(50.0±2.1)％비(12.0±1.3)％,P＜0.05]여종류배사인자(TNF)-α[(240.0 ±8.1)ng/L비(50.0±3.3) ng/L,P＜0.05]표체상승.결론 골육류류종류간세포고표체1,6분지N-당련유도거서세포M2표형전환.
Objective To investigate the effect of cancer stem-like cells (CSCs) from osteosarcoma cell line on the polarization of macrophages.Methods CSCs were pre-treated with different doses of swainsonine and co-cultured with macrophages,and the phenotypic specific markers on macrophages were detected respectively.Results Compared to the control group,the expression of Arg-1 [(12.0 ± 3.1) ％ vs.(40.0±2.6)％,P＜0.05] and interleukin (IL)-10 [(90.0±4.4) ng/Lvs.(150.0±6.8) ng/L,P ＜0.05] in macrophages co-cultured with CSCs pre-treated with swainsonine (1 mg/L) was decreased,whereas inducible nitric oxide synthase (iNOS) [(50.0 ±2.1)％ vs.(12.0 ± 1.3)％,P＜0.05] and tumor necrosis factor (TNF)-α [(240.0 ± 8.1) ng/L vs.(50.0 ± 3.3) ng/L,P ＜ 0.05] increased.Conclusion Increased expression of beta-1,6-oligosaccharide in CSCs derived from osteosarcoma cell line induced the differentiation of bone marrow-derived macrophages into anti-inflammatory M2 macrophages.
