CAJ | 학술논문

目的观察大鼠坐骨神经慢性卡压损伤后,结缔组织生长因子(CTGF)在背根神经节(DRG)的神经元中的表达变化.方法随机选取40只SD大鼠,采用经典的MACKINNON坐骨神经慢性卡压损伤模型,分别在正常及术后第2、4、6周时取大鼠L4 ～6 DRG神经细胞进行体外培养,应用免疫组织化学染色和逆转录-聚合酶链反应(RT-PCR)检测SD大鼠DRG神经细胞中CTGF的表达和合成水平.结果正常大鼠DRG神经元中有CTGF表达和合成.坐骨神经慢性卡压损伤后,DRG神经元中CTGF的表达水平在2、4、6周时分别为5.527 3±0.013 6、6.0567±0.141 5和4.533 3±0.0929;其合成水平分别为13.96±1.23、17.97±1.35和15.63±0.87.坐骨神经慢性卡压后DRG神经元中CTGF的表达和合成在2周时开始显著增加,4周时达到最高水平,6周时开始逐渐下降,但仍然显著高于正常水平.结论 CTGF参与周围神经慢性卡压损伤后DRG神经细胞的病理生理变化,可能与保护DRG的神经元有关.
목적 관찰대서좌골신경만성잡압손상후,결체조직생장인자(CTGF)재배근신경절(DRG)적신경원중적표체변화.방법 수궤선취40지SD대서,채용경전적MACKINNON좌골신경만성잡압손상모형,분별재정상급술후제2、4、6주시취대서L4 ～6 DRG신경세포진행체외배양,응용면역조직화학염색화역전록-취합매련반응(RT-PCR)검측SD대서DRG신경세포중CTGF적표체화합성수평.결과 정상대서DRG신경원중유CTGF표체화합성.좌골신경만성잡압손상후,DRG신경원중CTGF적표체수평재2、4、6주시분별위5.527 3±0.013 6、6.0567±0.141 5화4.533 3±0.0929;기합성수평분별위13.96±1.23、17.97±1.35화15.63±0.87.좌골신경만성잡압후DRG신경원중CTGF적표체화합성재2주시개시현저증가,4주시체도최고수평,6주시개시축점하강,단잉연현저고우정상수평.결론 CTGF삼여주위신경만성잡압손상후DRG신경세포적병리생리변화,가능여보호DRG적신경원유관.
Objective To investigate the expression of connective tissue growth factor (CTGF) in the nerve cells of dorsal root ganglion (DRG) after chronic sciatic nerve compression.Methods The chronic sciatic nerve constriction injury was made in 40 male SD rats by Mackinnon method.At the 0 week,2nd week,4th week and 6.th week after the operation,L4-L6 DRG that connected to sciatic nerve were harvested.The neurons were separated from the L4-L6 DRG.Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were applied to detect the expression and synthesis of CTGF in the neurons from the DRG.Results The expression and synthesis of CTGF were detected in the nerve cells of normal DRG.After chronic compression of the sciatic nerve,the CTGF expression level was 5.527 3 ± 0.013 6,6.056 7 ± 0.141 5 and 4.533 3 ± 0.092 9,respectively,and CTGF synthesis was 13.96 ± 1.23,17.97 ± 1.35 and 15.63 ±0.87,respectively.At the 2nd week after chronic compression of the sciatic nerve,the expression and synthesis of CTGF were increased dramatically.At the 4th week,the expression and synthesis of CTGF were increased obviously and reached the peak.At the 6th week,the expression and synthesis of CTGF were decreased slowly,but higher than on the normal side.Conclusion CTGF not only participates in the pathophysiological process of the nerve cells of DRG after the chronic compression of sciatic nerve,but also plays an important role in protecting the nerve cells.