中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
9期
1999-2001
,共3页
马昌科%沈忆新%孙涛%韩国霞%毛锦宁
馬昌科%瀋憶新%孫濤%韓國霞%毛錦寧
마창과%침억신%손도%한국하%모금저
拓扑%细胞行为%倾向性%聚二甲基硅氧烷
拓撲%細胞行為%傾嚮性%聚二甲基硅氧烷
탁복%세포행위%경향성%취이갑기규양완
Topography%Cell behaviors%Tendentiousness%Polydimethylsiloxane
目的 观察微纳米拓扑结构在体外模拟的人体环境下对细胞倾向性生长的影响.方法 制备宽度分别为12.5、25.0、50.0、75.0 μm的拓扑聚二甲基硅氧烷(PDMS)以及光滑的PDMS,分为实验组及对照组(E组),实验组分A组(12.5μm)、B组(25.0 μm)、C组(50.0 μm)及D组(75.0μm)4个亚组.按4种实验方式分别将成纤维细胞和神经胶质瘤细胞培养于各组PDMS上,观察细胞的形态、生长和分布,选择PDMS上、PDMS上方0～ 30μm范围内以及PDMS上方200～300 μm各5个视野,测量细胞长轴与水平线夹角α角度值,分为0～ 30°、31°～ 60°以及61°～ 90°3个等级,统计并分析测量结果.结果 各组细胞均沿PDMS沟槽结构生长,细胞α角大多数分布于0 ～30°;4个亚组中,A组和B组这一倾向性较C组及D组更明显.PDMS上方0～ 30 μm,只有B组细胞呈现0 ～ 30°的倾向性生长,以上差异均有统计学意义(P＜0.05).PDMS上方200 ～ 300μm,各组均无明显生长倾向性,差异无统计学意义(P＞0.05).结论 模拟人体的环境下,拓扑结构仍能影响细胞出现倾向性生长.宽度为12.5μm和25.0 μm的拓扑结构对细胞倾向性影响最明显,随着沟槽宽度的增加,对细胞倾向性的影响也减弱.
目的 觀察微納米拓撲結構在體外模擬的人體環境下對細胞傾嚮性生長的影響.方法 製備寬度分彆為12.5、25.0、50.0、75.0 μm的拓撲聚二甲基硅氧烷(PDMS)以及光滑的PDMS,分為實驗組及對照組(E組),實驗組分A組(12.5μm)、B組(25.0 μm)、C組(50.0 μm)及D組(75.0μm)4箇亞組.按4種實驗方式分彆將成纖維細胞和神經膠質瘤細胞培養于各組PDMS上,觀察細胞的形態、生長和分佈,選擇PDMS上、PDMS上方0～ 30μm範圍內以及PDMS上方200～300 μm各5箇視野,測量細胞長軸與水平線夾角α角度值,分為0～ 30°、31°～ 60°以及61°～ 90°3箇等級,統計併分析測量結果.結果 各組細胞均沿PDMS溝槽結構生長,細胞α角大多數分佈于0 ～30°;4箇亞組中,A組和B組這一傾嚮性較C組及D組更明顯.PDMS上方0～ 30 μm,隻有B組細胞呈現0 ～ 30°的傾嚮性生長,以上差異均有統計學意義(P＜0.05).PDMS上方200 ～ 300μm,各組均無明顯生長傾嚮性,差異無統計學意義(P＞0.05).結論 模擬人體的環境下,拓撲結構仍能影響細胞齣現傾嚮性生長.寬度為12.5μm和25.0 μm的拓撲結構對細胞傾嚮性影響最明顯,隨著溝槽寬度的增加,對細胞傾嚮性的影響也減弱.
목적 관찰미납미탁복결구재체외모의적인체배경하대세포경향성생장적영향.방법 제비관도분별위12.5、25.0、50.0、75.0 μm적탁복취이갑기규양완(PDMS)이급광활적PDMS,분위실험조급대조조(E조),실험조분A조(12.5μm)、B조(25.0 μm)、C조(50.0 μm)급D조(75.0μm)4개아조.안4충실험방식분별장성섬유세포화신경효질류세포배양우각조PDMS상,관찰세포적형태、생장화분포,선택PDMS상、PDMS상방0～ 30μm범위내이급PDMS상방200～300 μm각5개시야,측량세포장축여수평선협각α각도치,분위0～ 30°、31°～ 60°이급61°～ 90°3개등급,통계병분석측량결과.결과 각조세포균연PDMS구조결구생장,세포α각대다수분포우0 ～30°;4개아조중,A조화B조저일경향성교C조급D조경명현.PDMS상방0～ 30 μm,지유B조세포정현0 ～ 30°적경향성생장,이상차이균유통계학의의(P＜0.05).PDMS상방200 ～ 300μm,각조균무명현생장경향성,차이무통계학의의(P＞0.05).결론 모의인체적배경하,탁복결구잉능영향세포출현경향성생장.관도위12.5μm화25.0 μm적탁복결구대세포경향성영향최명현,수착구조관도적증가,대세포경향성적영향야감약.
Objective To discuss the influence of topography on tendentious growth of cell under conditions mimicking in vivo environments.Methods Polydimethylsiloxane (PDMS) with topography structure including the width of 12.5,25.0,50.0,75.0 μm respectively,and flat PDMS were made by heat moulding technology.The former is designed to trial group,and were divided into group A (12.5 μm),group B (25.0 μm),group C (50.0 μm) and group D (75.0 μm),respectively.The latter is designed to the control groupE.According to 4 patterns put cells on the different PDMS.Observed the shape,growth and distribution of cells.Photoed 5 figures on PDMS,over PDMS 0-30 μm and 200-300 μm,respectively.Measured the angleαthat made up of long axis of cells and horizontal line,and were designed to three levels based on 0-30°,31°-60°,61°-90°.The available data were extracted and analyzed with SPSS 17.0 software.Results On the PDMS,cells of trial group grow according to topography in 4 patterns,most angleα distributed in the 0-30°.In addition,this tendency of group A and B was more significant than group C and D.Over the PDMS 0-30 μm,cells of trial group appeared tendentious 0-30° growth in pattern 2 only.All difference were statistically significant (P ＜ 0.05).Over the PDMS 200-300 μm,all did not appear tendentious growth in pattern2,3 and 4,the difference was not statistically significant (P ＞0.05).Conclusion Under conditions mimicking in vivo environments,the influence of topography on tendentious growth of cell still persisted,and this influence was more weaker along with the increase of the width,the width of 12.5 μm and 25.0 μm were most significant in this trial.
