CAJ | 학술논문

目的观察CD13在骨瘤及骨肉瘤中的表达及意义.方法免疫组织化学法检测20例良性骨瘤及56例骨肉瘤组织中CD13蛋白的表达,逆转录-聚合酶链反应(RT-PCR)检测CD13mRNA在20例骨肉瘤及相应肉瘤旁组织中的表达,分析CD13表达与临床病理参数的关系.结果免疫组织化学结果显示CD13蛋白在良性骨瘤及骨肉瘤组织中的阳性表达率分别为64.3％、10.0％,差异有统计学意义(P＜0.05).CD13蛋白在Ⅲ期及Ⅰ～Ⅱ期骨肉瘤组织中的阳性表达率分别为90.0％、58.9％,差异有统计学意义(P＜0.05),而与患者的年龄、性别、肿瘤发生部位、肿瘤大小无明显相关(P＞0.05).RT-PCR结果显示CD13 mRNA在肉瘤中的表达明显高于肉瘤旁组织(P＜0.05).结论 CD13的表达与骨肉瘤发生及进展明显相关.
목적 관찰CD13재골류급골육류중적표체급의의.방법 면역조직화학법검측20례량성골류급56례골육류조직중CD13단백적표체,역전록-취합매련반응(RT-PCR)검측CD13mRNA재20례골육류급상응육류방조직중적표체,분석CD13표체여림상병리삼수적관계.결과 면역조직화학결과현시CD13단백재량성골류급골육류조직중적양성표체솔분별위64.3％、10.0％,차이유통계학의의(P＜0.05).CD13단백재Ⅲ기급Ⅰ～Ⅱ기골육류조직중적양성표체솔분별위90.0％、58.9％,차이유통계학의의(P＜0.05),이여환자적년령、성별、종류발생부위、종류대소무명현상관(P＞0.05).RT-PCR결과현시CD13 mRNA재육류중적표체명현고우육류방조직(P＜0.05).결론 CD13적표체여골육류발생급진전명현상관.
Objective To investigate the expression and significance of CD13 in osteoma and osteosarcoma.Methods Immunohistochemistry was used to detect the protein expression of CD13 in 20 cases of benign osteoma and 56 cases of osteosarcoma.reverse transcription-polymerase chain reactionn (RT-PCR) method was used to examine the level of CD13 mRNA in 20 cases of osteosarcoma and corresponding adjacent tissue,and the relationship between expression of CD13 and clinicopathological parameters was evaluated.Results Immunohistochemistry showed the positive rate of CD13 protein in benign osteoma and osteosarcoma tissues was 64.3％ and 10.0％ respectively (P ＜ 0.05).CD13 protein in osteosarcoma tissues of phase Ⅲ and phase Ⅰ-Ⅱ was 90.0％ and 58.9％ respectively (P ＜ 0.05).The CD13 protein expression was not correlated with age,sex,tumor location,and tumor size (P ＞ 0.05).RT-PCR results showed CD13 mRNA expression level was significantly higher in sarcoma than that in the adjacent tissue (P ＜0.05).Conclusion CD13 is obviously correlated with the occurrence and progression of osteosarcoma.