CAJ | 학술논문

目的探讨膜联蛋白A5(ANXA5)在肝细胞肝癌患者外周血及癌组织中的表达及其与临床病理特征之间的关系.方法将148例患者分为肝细胞肝癌组及正常对照组,其中肝细胞肝癌组82例,正常对照组66例,包括肝血管瘤患者47例、肝内胆管结石7例、肝挫裂伤12例;收集两组患者的血清及组织标本,用酶联免疫吸附试验(ELISA)法检测两组患者外周血中ANXA5的含量,免疫组织化学法检测组织标本中ANXA5的表达.结果肝细胞肝癌患者血清中ANXA5的含量为(46.22 ±21.02) μg/L,明显高于正常对照组的(33.13 ±12.44) μg/L(P＜0.05),肝细胞肝癌组中ANXA5的阳性表达率为68.29％,正常对照组为22.72％(P＜0.05);甲胎蛋白(AFP)值较高、分化程度较低、肿瘤直径较大、感染乙型肝炎病毒(HBV)以及发生复发转移的肝癌患者癌组织中ANXA5的表达显著升高(P＜0.05);但患者血清中ANXA5的浓度与AFP浓度之间无明显相关(P＞0.05).结论 ANXA5的高表达可能与肝细胞肝癌的发生发展密切相关.
목적 탐토막련단백A5(ANXA5)재간세포간암환자외주혈급암조직중적표체급기여림상병리특정지간적관계.방법 장148례환자분위간세포간암조급정상대조조,기중간세포간암조82례,정상대조조66례,포괄간혈관류환자47례、간내담관결석7례、간좌렬상12례;수집량조환자적혈청급조직표본,용매련면역흡부시험(ELISA)법검측량조환자외주혈중ANXA5적함량,면역조직화학법검측조직표본중ANXA5적표체.결과 간세포간암환자혈청중ANXA5적함량위(46.22 ±21.02) μg/L,명현고우정상대조조적(33.13 ±12.44) μg/L(P＜0.05),간세포간암조중ANXA5적양성표체솔위68.29％,정상대조조위22.72％(P＜0.05);갑태단백(AFP)치교고、분화정도교저、종류직경교대、감염을형간염병독(HBV)이급발생복발전이적간암환자암조직중ANXA5적표체현저승고(P＜0.05);단환자혈청중ANXA5적농도여AFP농도지간무명현상관(P＞0.05).결론 ANXA5적고표체가능여간세포간암적발생발전밀절상관.
Objective To investigate the expression of annexin A5 (ANXA5) in the peripheral blood and tumor tissues of patients with hepatocellular carcinoma (HCC) and its relation with the clinicopathologic characteristics of the carcinoma.Methods 148 patients were divided into HCC group (82 cases) and normal control group (66 cases).In normal control group,there were 47 cases of liver hemangioma,7 cases of hepatolithiasis,and 12 cases of liver injury.The serum from the peripheral blood and tissue samples were collected.Serum concentration of ANXA5,and the expression of ANXA5 in tissues were analyzed by immunohistochemistry.Results ANXA5 level in serum of HCC patients was (46.22 ± 21.02) μg/L,significantly higher than in control group [(33.13 ± 12.44) μg/L] (P ＜ 0.05).The ANXA5 positive expression rate in HCC and control tissues was 68.29％ and 22.72％ respectively (P ＜0.05).In the patients with higher alpha fetal protein (AFP) level,larger tumor size,low differentiation degree,hepatitis B virus (HBV) inffection,recurrence and metastasis,the expression of ANXA5 in the HCC tissues was significantly higher (P ＜ 0.05).But there was no obvious correlation between the ANXA5 concentration and AFP concentration in the serum of patients with HCC (r =0.161,P ＞ 0.05).Conclusion The low expression of ANXA5 is closely related to the tumor progression.Detection of the serum concentration of ANXA5 can be used to diagnose HCC and predict the progression of liver cancer.