CAJ | 학술논문

目的观察乙肝相关性肝移植受者术前体外培养的单核细胞来源的树突状细胞(DC)免疫表型、功能变化.方法通过DC生成培养基体外诱导健康人(对照组)和拟行肝移植的乙肝患者(患者组)DC,并分别负载乙肝表面抗原(HBsAg)和乙肝核心抗原(HBcAg)诱导成熟,比较两组DC形态、表型、刺激淋巴细胞的增殖能力及培养上清中白细胞介素(IL)-12、IL-10分泌量的变化,并检测患者组中负载HBsAg和HBcAg抗原成熟的DC(负载组,n=9)与未负载抗原成熟的DC(未负载组,n=9)诱导辅助性T细胞(Th)极化的能力.结果患者组和对照组的CD83阳性率分别为(53.10±16.23)％、(81.91±9.20)％ (P ＜0.01),人类白细胞DR抗原(HLA-DR)及CD40/CD80/CD86等共刺激信号分子阳性率比较差异无统计学意义(P＞0.05).患者组DC刺激自体T细胞增殖能力降低(P＜0.05).患者组IL-12、IL-10浓度分别为(141.94±58.81)、(12.86±5.72) ng/L,与健康组比较差异无统计学意义(P＞0.05).未负载组与负载组诱导T细胞极化(Th1)比例分别为(7.59±2.62)％、(11.17±1.11)％(P＜0.05).结论乙肝相关性肝病肝移植患者术前DC的表型、功能受到一定的抑制,但在体外有效的负载抗原后亦能有效的诱导Th1应答,从而激活细胞免疫,提高患者主动免疫力.
목적 관찰을간상관성간이식수자술전체외배양적단핵세포래원적수돌상세포(DC)면역표형、공능변화.방법 통과DC생성배양기체외유도건강인(대조조)화의행간이식적을간환자(환자조)DC,병분별부재을간표면항원(HBsAg)화을간핵심항원(HBcAg)유도성숙,비교량조DC형태、표형、자격림파세포적증식능력급배양상청중백세포개소(IL)-12、IL-10분비량적변화,병검측환자조중부재HBsAg화HBcAg항원성숙적DC(부재조,n=9)여미부재항원성숙적DC(미부재조,n=9)유도보조성T세포(Th)겁화적능력.결과 환자조화대조조적CD83양성솔분별위(53.10±16.23)％、(81.91±9.20)％ (P ＜0.01),인류백세포DR항원(HLA-DR)급CD40/CD80/CD86등공자격신호분자양성솔비교차이무통계학의의(P＞0.05).환자조DC자격자체T세포증식능력강저(P＜0.05).환자조IL-12、IL-10농도분별위(141.94±58.81)、(12.86±5.72) ng/L,여건강조비교차이무통계학의의(P＞0.05).미부재조여부재조유도T세포겁화(Th1)비례분별위(7.59±2.62)％、(11.17±1.11)％(P＜0.05).결론 을간상관성간병간이식환자술전DC적표형、공능수도일정적억제,단재체외유효적부재항원후역능유효적유도Th1응답,종이격활세포면역,제고환자주동면역력.
Objective To investigate the immune Phenotype and function of monocyte-derived dendritic cell (DCs) cultured in vitro from hepatitis B virus (HBV) correlated liver transplant (LT) recipients who were in the situation before operation.Methods The DCs of healthy control group and HBV correlated LT recipients before operation were induced by DC culture medium respectively,and then incubated with hepatitis B virus surface antigen (HBsAg) and hepatitis B virus core antigen (HBcAg) to induce matured DCs.Compared the DC morphology and Phenotype and Proliferative capacity and the concentration of Interleukin (IL)-12 and IL-10 in supernatants in each group (control group n =7,patient group n =9),Meanwhile compared the capacity of DCs induced polarization of T cell between incubated group and non-incubated group.Results The rate of CD83 was (53.10 ± 16.23) in patient group,(81.91 ± 9.20) in control group respectively (P ＜0.01).Meanwhile,the difference among HLA-DR and CD40/ CD80/CD86 were not significant (P ＞ 0.1).The proliferative capacity of DC were reduced in LT recipients (P ＜0.05).There was no significant difference in the concentration of IL-12 and IL-10 were (141.94 ± 58.81) ng/L and (12.86 ± 5.72) ng/L in patient group respectively.The rate of Th1 induced by DCs was (7.59 ± 2.62) in non-incubated group,and (11.17 ± 1.11) in incubated group respectively (P ＜ 0.05).Conclusion The immune phenotype and function of DCs were inhibited in LT recipients before operation,but it could induce Th1 response efficiently after incubated with HBsAg and HBcAg in vitro,and increased active immunity.